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Evaluation of three native entomopathogenic nematodes (Rhabditidae) against third instar larvae of Phyllophaga menetriesi (Coleoptera: Scarabaeidae)

Published online by Cambridge University Press:  01 December 2006

Paulina Quintero-Marin ,
Ana M. Caicedo ,
James Montoya-Lerma  and
Andreas Gaigl
Paulina Quintero-Marin
Affiliation:
Biology Department, Universidad del Valle, Cali, Apartado Aéreo 25360, Colombia Centre Internacional de Agricultura Tropical (CIAT), Apartado Aéreo 6713, Cali, Colombia
Ana M. Caicedo
Affiliation:
Biology Department, Universidad del Valle, Cali, Apartado Aéreo 25360, Colombia Centre Internacional de Agricultura Tropical (CIAT), Apartado Aéreo 6713, Cali, Colombia
James Montoya-Lerma
Affiliation:
Biology Department, Universidad del Valle, Cali, Apartado Aéreo 25360, Colombia
Andreas Gaigl*
Affiliation:
Faculdad de Agronomia, Universidad Nacional de Colombia, Ciudad Universitaria, Edificio 500, Bogotá, Colombia
*
* E-mail: agaigl@unal.edu.co or agaigl@gmail.com
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Abstract

The infectivity and pathogenicity of three native entomopathogenic nematodes (Heterorhabditis (HNI 0100 and sp-CIAT) and Steinernema (SNI 0198)) were evaluated in third instar larvae of Phyllophaga menetriesi (Blanchard) under laboratory conditions. Concentrations of 7000 and 13,000 infective juveniles per millilitre were applied to the coleopteran larval host (individuals) that had been placed in plastic cups with 40 g of sterile sand at 5.8% (w/w) moisture. The evaluations were done 5 and 10 days after treatment. It was observed that both the percentage of infectivity (74.5%, n = 247), measured in terms of percentage of penetration and the percentage of pathogenicity (10.5%, n = 247) of the three native nematodes were significantly different due to the treatments. Steinernema had the highest values of infectivity (>80%), but no pathogenicity. In contrast, the treatments with Heterorhabditis, despite having the lowest degree of infectivity (52.9%), had the highest mortality values (31.6%). Therefore, the Heterorhabditis strains were considered to be the most promising as biological control organisms within an integrated pest management programme due to their higher degree of pathogenicity. Nevertheless, different strains and species of native nematodes should be evaluated in all the developmental stages of the pest.

La capacité d'infection et le pouvoir pathogène des trois nématodes entomopathogènes indigènes (Heterorhabditis (HNI 0100 et sp-CIAT) et Steinernema (SNI 0198)) ont été évalués sur des larves de troisieme stada de Phyllophaga menetriesi (Blanchard) en conditions de laboratoire. Des concentrations de 7000 à 13000 juvéniles infectieux/ml ont été appliquées sur les larves du coléoptère hôte, placées dans des gobelets en plastique avec 40 g de sable stérile à 5,8% (w/w) d'humidité. Les observations ont été réalisées 5 et 10 jours après le traitement. On a constaté qu' aussi bien la capacité d'infection (74,5%; n=247), mesuré en termes de pourcentage de pénétration, que le pouvoir pathogène (10,5%; n=247) des trois nématodes indigènes, sont significativement différents. Steinernema a la capacite d'infection la plus élevée (> 80%) mais pas de pouvoir pathogène. Par contre, les Heterorhabditis qui montrent la capacite d'infection la plus faible (52,9%) ont les taux de mortalité les plus élevés (31,6%). Aussi, les souches de Heterorhabditis sont considérées comme les plus prometteuses comme agent de lutte biologique dans le cadre d'un programme de lutte intégrée contre le ravageur du fait de leur pouvoir pathogène élevé. Néanmoins, différentes souches et espèces de nématodes indigènes devront être testées sur tous les stades de développement du ravageur.

Keywords

lutte biologiqueHeterorhabditisSteinernemaVerts blancsPhyllophaga menetriesibiologique controlHeterorhabditisSteinernemaVerts blancsPhyllophaga menetriesi
Type
Research Paper
Information
International Journal of Tropical Insect Science , Volume 26 , Issue 4 , December 2006 , pp. 233 - 238
Copyright
Copyright © ICIPE 2006

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