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Heterogeneity in the Leishmania donovani complex (Protozoa: Kinetoplastidae)

Published online by Cambridge University Press:  19 September 2011

N. N. Massamba  and
M. J. Mutinga
N. N. Massamba*
Affiliation:
The International Centre of Insect Physiology and Ecology (ICIPE)P. O. Box 30772, Nairobi, Kenya
M. J. Mutinga
Affiliation:
The International Centre of Insect Physiology and Ecology (ICIPE)P. O. Box 30772, Nairobi, Kenya
*
* Author to whom correspondence should be addressed.
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Abstract

Restriction analysis of total DNAs from Leishmania donovani complex strains revealed heterogeneous distribution of kDNA fragments among the reference strains of L. donovani. The cloned kDNA fragments used as probes in the hybridisation analysis have proven useful for identifying and characterising L. donovani subspecies when screening newly isolated leishmanial parasites from patients, vectors and reservoir hosts. This genetic heterogeneity was confirmed by karyotyping by pulsed field gel electrophoresis of four L. donovani reference strains. A high degree of polymorphism was shown, affecting not only the chromosome size, but also their number.

Résumé

L'analyse au moyen des enzymes de restriction des ADNs génomiques provenant des souches du complexe Leishmania donovani a montré une distribution hétérogène des fragments des ADNs kinétoplastiques entre les souches de reférence de L. donovani. Les fragments clonés des ADNs kinétoplastiques utilisés comme sondes dans les expériences d'hybridation se sont révelés utiles dans l'identification et la caractérisation des souches de L. donovani au cours de l'analyse des stocks de Leishmania nouvellement isolés des patients, des vecteurs et des animaux hôtes. Cette hétérogénéité a été confirmée par l'analyse des chromosomes (karyotypes) au moyen de l'électrophorèse à champ pulsé de quatre souches de reférence de L. donovani où un haut degré de polymorphisme touchant non seulement à la grandeur des chromosomes mais aussi à leur nombre a été observé.

Keywords

heterogeneityLeishmania donovani complexkinetoplast DNAchromosomekaryotypehétérogénéitécomplexe Leishmania donovaniADN kinétoplastiquechromosomekaryotype
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 15 , Issue 4-5 , October 1994 , pp. 541 - 550
Copyright
Copyright © ICIPE 1994

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References

REFERENCES

Al-Taqi, M. and Evans, F. A. (1978) Characterisation of Leishmania spp. from Kuwait by isoenzyme electrophoresis. Trans. R. Soc. Trop. Med. Hyg. 72, 56–65.CrossRefGoogle ScholarPubMed
Aljeboori, T. I. and Evans, D. A. (1980) Leishmania spp. in Iraq. Elecophoretic isoenzyme patterns 1. Visceral leishmaniasis. Trans. R. Soc. Trop. Med. Hyg. 74, 169–177.CrossRefGoogle ScholarPubMed
Ashford, R. W. (1986) In Leishmania, Taxonomie et Phylogenèse: Applications Eco-Epidemiologiques. (Collection internationale CNRS/INSERM 1984) pp. 257–264. IMEEE, Montpellier.Google Scholar
Birboin, C. and Doly, J. (1979) Rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7, 1515–1523.Google Scholar
Bishop, R. P. and Akinsehinwa, F. (1989) Characterisation of Leishmania donovani stocks by genomic DNA heterogeneity and molecular karyotype. Trans. R. Soc. Trop. Med. Hyg. 83, 629–634.CrossRefGoogle ScholarPubMed
Bishop, R. P. and Miles, M. A. (1987) Chromosome size polymorphisms of Leishmania donovani. Mol. Biochem. Parasitol. 24, 263–272.CrossRefGoogle ScholarPubMed
Codere, J. A., Beverley, S. M., Schmike, R. T. and Santi, D. (1993) Overproduction of bifunctional thymidylate-synthetase dihydrofolate reductase and DNA amplification in methotrexate-resistant Leishmania tropica. Proc. Natl. Acad. Sci. (USA) 80, 2132–2136.CrossRefGoogle Scholar
Comeau, A. M., Miller, S. I. and McMahon-Pratt, D. (1987) Chromosome location of four genes in Leishmania. Mol. Biochem. Parasitol. 21, 161–169.CrossRefGoogle Scholar
Denhardt, D. T. (1966) A membrane-filter technique for the detection of complementary DNA. Biochem. Biophys. Res. Comm. 23, 641–646.CrossRefGoogle ScholarPubMed
Detke, S., Katakura, K. and Chang, K. P. (1989) DNA amplification in arsenite-resistant Leishmania. Exptl. Cell Res. 100, 161–171.CrossRefGoogle Scholar
Dujardin, J. C., Gajendran, N., Arevalo, J., Llanos-Cuentas, A., Guerra, H., Gomez, J., Arroyo, J., De Doncker, S., Jacquet, D., Hamers, R. and Le Ray, D. (1993) Karyotype polymorphisms and conserved characters in the Leishmania (Viannia) brazilensis complex explored with chromosome derived probes. Ann. Soc. Belge Med. Trop. 73, 101–128.Google Scholar
Ellis, J. T. and Crampton, J. M. (1991) Differences between Leishmania (Leishmania) chagasi, L. (L.) infantum and L. (L.) donovani as shown by DNA fingerprinting. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, 86, 479–481.CrossRefGoogle ScholarPubMed
Gramiccia, M., Smith, D. F., Angelici, M. C., Ready, P. D. and Gradoni, L. (1992) A kinetoplast DNA probe diagnostic for Leishmania infantum. Parasitology 105, 29–34.CrossRefGoogle ScholarPubMed
Greenblatt, C., Schnur, L. F., Juster, R. and Sulitzeanu, A. (1990) Clonal heterogeneity in populations of Leishmania major. Isr. J. Med. Sci. 26, 129–135.Google ScholarPubMed
Grimaldi, G., David, J. R. and McMahon-Pratt, D. (1987) Identification and distribution of New World Leishmania species characterized by serodeme analysis using monoclonal antibodies. Am. J. Trop. Med. Hyg. 36, 270–287.CrossRefGoogle ScholarPubMed
Grimaldi, G., Tesh, R. B. and McMahon-Pratt, D. (1989) A review of the geographical distribution and epidemiology of leishmaniasis in the New World. Am. J. Trop. Med. Hyg. 41, 687–725.CrossRefGoogle ScholarPubMed
Handman, E., Hocking, R. E., Mitchell, G. F. and Spithill, T. W. (1983) Isolation and characterisation of infective and non-infective clones of Leishmania tropica. Mol. Biochem. Parasitol. 7, 111–126.CrossRefGoogle ScholarPubMed
Hu, X., Lu, H., Luo, P., Wang, Z., Hu, X. and Yi, T. (1992) Identification of Leishmania donovani isolates from different kala-azar foci in China by kDNA hybridisation. Chin. Med. Sci. J. 7, 63–66.Google Scholar
Lainson, R., Miles, M. A. and Shaw, J. J. (1981) On the identification of viscerotropic leishmaniasis. Ann. Trop. Med. Parasitol. 75, 252–253.CrossRefGoogle Scholar
Le Blancq, S. M. and Peters, W. (1986) Leishmania in the Old World: 4. The distribution of L. donovani sensu lato zymodemes. Trans. R. Soc. Trop. Med. Hyg. 80, 367–377.CrossRefGoogle ScholarPubMed
Lee, S. Y., Lee, S. T. and Chang, K. P. (1992) Transkinetoplastidy—A novel phenomenon involving bulk alterations of mitochondrion-kinetoplast DNA of a trypanosomatid protozoan. J. Protozool. 39, 190–196.CrossRefGoogle ScholarPubMed
Maniatis, T., Fritsch, E. and Sambrook, J. (1982) Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.Google Scholar
Mansour, N. S., Stauber, L. A. and McCoy, J. R. (1970) Characterisation of Leishmania donovani strains from the Sudan, Kenya and Mediterranean area in domestic dogs. J. Parasitol. 56, 468–472.CrossRefGoogle Scholar
Massamba, N. N. and Mutinga, M. J. (1992) Recombinant kinetoplast DNA (kDNA) probe for identifying Leishmania tropica. Acta Tropica 52, 1–15.CrossRefGoogle ScholarPubMed
Massamba, N. N., Mutinga, M. J. and Odero, B. N. (1993) Characterisation of Kenyan isolates of Leishmania by cellulose acetate electrophoresis. Insect Sci. Applic. 14, 153–161.Google Scholar
Mebrahtu, Y. M., Lawyer, P. G., Pamba, H., Koech, D., Perkins, P. V., Roberts, C. R., Were, J. B. and Hendricks, L. D. (1992) Biochemical characterisation and zymodeme classification of Leishmania isolates from patients, vectors and reservoir hosts in Kenya. Am. J. Trop. Med. Hyg. 47, 852–892.CrossRefGoogle ScholarPubMed
Mutinga, M. J., Ngoka, J. M., Schnur, L. F. and Chance, M. L. (1980) The isolation and identification of leishmanial parasites from dogs in Machakos District of Kenya, and possible role of dogs as reservoirs of kala-azar in East Africa. Ann. Trop. Med. Parasitol. 74, 139–144.CrossRefGoogle ScholarPubMed
Mutinga, M. J., Mutero, C. M., Ngindu, A. and Amimo, F. A. (1988) The isolation of leishmanial parasites from domestic goats and wild hosts and possible role of goats as reservoirs of leishmaniasis. Insect Sci. Applic. 9, 339–344.Google Scholar
Mutinga, M. J., Kiharas, M., Lohding, A., Mutero, C. M., Ngatia, T. A. and Karann, F. (1989) Leishmaniasis in Kenya: Description of leishmaniasis of adomestic goat from Transmara, Narok District, Kenya. Trop. Med. Parasitol. 40, 91–96.Google Scholar
Navarro, M., Maingon, R., Hamers, R. and Segovia, M. (1992) Dynamics and size polymorphisms of mimchromosomes in Leishmania major LV-561 cloned lines. Mol. Biochem. Parasitol. 55, 197–206.CrossRefGoogle ScholarPubMed
Okot-Kotber, B. M. (1985) A rapid chromatographic method for elimination of fungal contamination in “in vitro” cultures of Leishmania spp. Parasitology 91, 1–7.CrossRefGoogle ScholarPubMed
Okot-Kotber, B. M., Mutinga, M. J. and Kaddu, J. B. (1989) Biochemical characterisation of Leishmania spp. isolated from man and wild animals in Kenya. Int. J. Parasitol. 19, 657–663.CrossRefGoogle ScholarPubMed
Pacheco, R. S., Grimaldi, G. Jr, Momen, H. and Morel, C. N. (1990) Population heterogeneity among clones of New World Leishmania species. Parasitology 100, 393–398.CrossRefGoogle ScholarPubMed
Pacheco, R. S., Lopes, U. G., Morel, C. M., Grimaldi, G. and Momen, H. (1986) Schizodeme analysis of Leishmania isolates and comparison with some phenotypic techniques. In International Symposium on Taxonomy and Phylogeny of Leishmania (Edited by Rioux, J. A.), pp. 57–65. IMEE, Montpellier.Google Scholar
Pages, M., Bastien, P., Veas, F., Rossi, V., Bellis, M., Wincker, P., Rioux, J. A. and Roizes, G. (1989) Chromosome size and number polymorphisms in Leishmania infantum. Mol. Biochem. Parasitol. 24, 161–168.CrossRefGoogle Scholar
Palatnik, C. B., Previato, J. O., Mendonca-Previato, L. and Borojevic, R. (1990) A new approach to the phylogeny of Leishmania species specificity of glycoconjugates ligands for promastigote internalization into murine macrophages. Parasitol. Res. 76, 289–293.CrossRefGoogle Scholar
Perkins, P. V., Githure, J. I., Mebrahtu, Y., Kiilu, G., Anjili, C., Ngumbi, P. S., Nzovu, J., Oster, C. N., Whitmire, R. E., Leevwemberg, J., Hendricks, L. D. and Koech, D. K. (1988) Isolation of Leishmania donovani from Phlebotomus martini in Baringo District, Kenya. Trans. R. Soc. Trop. Med. Hyg. 83, 695–700.CrossRefGoogle Scholar
Rigby, P. W. J., Dickmann, M., Rhodes, C. and Berg, P. J. (1977) Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with polymerase I. J. Mol. Biol. 113, 237–251.CrossRefGoogle ScholarPubMed
Shaw, J. J., Ishikawa, E. A., Lainson, R., Braga, R. R. and Silveira, F. T. (1991) Cutaneous leishmaniasis of man due to Leishmania (Viannia) shawi Lainson, de Souza, Povoa, Ishikawa & Silveira, in Para State, Brazil. Ann. Parasitol. Hum. Comp. 66(6), 243–246.CrossRefGoogle Scholar
Schnur, L. F., Chance, M. L., Ebert, F., Thomas, S. C. and Peters, W. (1981) The biochemical and serological taxonomy of visceralizing Leishmania. Ann. Trop. Med. Parasitol. 75, 131–144.CrossRefGoogle Scholar
Schwartz, D. C. and Cantor, C. R. (1984) Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell 37, 67–75.CrossRefGoogle ScholarPubMed
Southern, E. M. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503–517.CrossRefGoogle ScholarPubMed
Stauber, L. A. (1966) Characterisation of strains of Leishmania donovani. Exptl. Parasitol. 18, 1–11.CrossRefGoogle ScholarPubMed
Stuart, K. D. (1991) Circular and linear multicopy DNAs in Leishmania. Parasitology Today 7, 158–159.CrossRefGoogle ScholarPubMed
Van Der Ploeg, L. H. T., Cornelissen, A. W. C. A., Barry, J. D. and Borst, P. (1984a) The chromosomes of kinetoplastida. Embo J. 3, 3109–3115.CrossRefGoogle ScholarPubMed
Van Der Ploeg, L. H. T., Schwartz, D. C., Cantor, C. R. and Borst, P. (1984b) Antigenic variation in Trypanosoma brucei analysed by electrophoretic separation of chromosome-sized DNA molecules. Cell 37, 77–84.CrossRefGoogle Scholar
Wilson, K., Beverley, S. M. and Ullman, B. (1992) Stable amplification of linear extra chromosomal DNA in mycophenolic acid-resistant Leishmania donovani. Mol. Biochem. Parasitol. 55, 197–206.CrossRefGoogle Scholar
Wirth, D. F., Rogers, W. O., Barker, R., Dourado, H., Suesebang, L. and Alburqueque, B. (1990) Leishmaniasis and malaria: DNA probes for diagnosis and epidemiologic analysis. Ann. New York Acad. Sci. 569, 183–192.CrossRefGoogle Scholar