Hostname: page-component-78c5997874-m6dg7 Total loading time: 0 Render date: 2024-11-13T03:24:44.628Z Has data issue: false hasContentIssue false
  • English
  • Français

Host immunoglobulin G in the haemolymph of the brown ear tick, Rhipicephalus appendiculatus (Neumann, 1901)

Published online by Cambridge University Press:  19 September 2011

S. K. Mbogo ,
E. O. Osir  and
A. O. Mongi
S. K. Mbogo
Affiliation:
Kenya Agricultural Research Institute, National Veterinary Research Centre, P. O. Box 32, Kikuyu
E. O. Osir
Affiliation:
The International Centre of Insect Physiology and Ecology (ICIPE), P. O. Box 30772, Nairobi
A. O. Mongi
Affiliation:
The International Centre of Insect Physiology and Ecology (ICIPE), P. O. Box 30772, Nairobi
Get access

Abstract

Adult female Rhipicephalus appendiculatus ticks were fed on rabbits immunized with either bovine serum albumin, solubilized tick midgut membrane proteins, a 50:50 mixture of bovine serum albumin and solubilized tick midgut membrane proteins or buffer. Haemolymph harvested from these ticks after they had fed to engorgement and sera from the immunized rabbits were assayed for the presence and quantity of both the total rabbit IgG and specific rabbit IgG raised against bovine serum albumin using the enzyme linked immunosorbent assay technique. The results obtained indicated that rabbit IgG was able to cross the midgut barrier of the tick into the haemolymph. Furthermore, the amounts of IgG crossing into the haemolymph were dependent on the antibody titres in the serum. The results also showed that feeding ticks on blood containing anti-tick midgut antibodies increased the passage of IgG into the haemolymph.

Résumé

Des tiques Rhipicephalus appendiculatus femelles ont été nourries sur des lapins immunisés à partir soit d'albumine sérique bovin, soit de proteines solubles issues de la membrane intestinale de tiques, soi d'un mélange à égale des deux précédentes substances ou à partir d'une solution tampon. Il a été procédé a moyen de la technique de l'ELISA au dosage de l'IgG des lapins dans l'hémolymphe des tiques de même qu'à l'estimation de la quantité d'hemoglobine secrétée par les lapins contre les substances à eux injectées. Les résultats obtenus, indiquent que l'IgG des lapins peut traverser la barrière intestinale de la tique et se retrouver dans l'hemolymphe.Ils indiquent aussi que le taux d'IgG traversant la barrière intestinale de la tique dépend du taux d'anticorps sérique. Ces résultats de la même manière indiquent que la prise de nourriture par des tiques sur des animaux immuns contre des proteines de tiques d'origine intestinale endommage les structures intestinales et augmente le passage des anticorps de l'intestine à l'hémolymphe.

Keywords

Enzyme linked immunosorbent assayimmunizationIgG in tick haemolymphRhipicephalus appendiculatussolubilized tick midgut membrane proteinsRhipicephalus appendiculatusimmunoglobulines G dans l'hémolymphe de tiquesELISAproteines solubles obtenues a partir de la membrane intestinale de tiquesimmunisation
Type
Tick Immunology
Information
International Journal of Tropical Insect Science , Volume 13 , Special Issue 4: Status and Recent Advances in Tick Management in Africa , August 1992 , pp. 481 - 485
Copyright
Copyright © ICIPE 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Ackerman, S., Brian Clare, F., McGill, T. W. and Sonenshine, D. E. (1981) Passage of host serum components, including antibody, across the digestive tract of Dermacentor variabilis (Say). J. Parasitol. 67, 737–740.CrossRefGoogle ScholarPubMed
Agbede, R. I. S. and Kemp, D. H. (1986) Immunization of cattle against Boophilus microplus using extracts derived from adult female ticks: Histopathology of ticks feeding on vaccinated cattle. Int. J. Parasitol. 16, 35–41.CrossRefGoogle ScholarPubMed
Akov, S. (1982) Blood digestion in ticks. In Physiology of Ticks (Edited by Obenchain, F. D. and Galun, R.), pp. 197–211. Pergamon Press, Oxford.CrossRefGoogle Scholar
Bailey, K. P. (1960) Notes on the rearing of Rhipicephalus appendiculatus and their infection with Theileria parva for experimental transmission. Bull. Epizoot. Dis. Afr. 8, 33–43.Google Scholar
Balashov, Y. S. (1972) Blood-sucking tick (Ixodidea) vectors of diseases of man and animals. Misc. Publ. entomol. soc. Am. 8, 161–376.Google Scholar
Ben-Yakir, D., Fox, C. J., Homer, J. T. and Barker, R. W. (1987) Quantification of hostimmunoglobulin G in the haemolymph of ticks. J. Parasitol. 73, 669–671.CrossRefGoogle Scholar
Ben-Yakir, D. (1989) Quantitative studies of host immunoglobulin G in the haemolymph of ticks. J. med. entomol. 26, 243–246.CrossRefGoogle ScholarPubMed
Ben-Yakir, D., Carl Fox, J., Homer, J. T. and Barker, R. W. (1986) Quantitative studies of host immunoglobulin G passage into the hemocoel of the ticks Amblyomma americanum and Dermacentor variabilis. In Morphology, Physiology and Behavioural Biology of Ticks (Edited by Sauer, J. R. and Hair, J. A.), pp. 329– 341. Ellis Horwood, Chichester.Google Scholar
Berdyer, A. and Khudainazarova, S. N. (1976) A study of acquired resistance to adults of Hyalomma asiaticum asiaticum in experiment on lambs. Parasitologiya 10, 619–625.Google Scholar
Bram, R. A. (1975) Tick-borne livestock diseases and their vectors. I. The global problem. Wld. A. Rev. 16, 1–5.Google Scholar
Chinzei, Y. and Minoura, H. (1987) Host immunoglobulin G litre and antibody activity in haemolymph of the tick, Ornithodoros moubata. Med. Vet. Entomol. 1, 409–416.CrossRefGoogle Scholar
Dolan, T. T. and Young, A. S. (1981) An approach to the economic assessment of East Coast fever in Kenya. In Advances in the Control (Edited by Irvin, A. D., Cunningham, M. P. and Young, A. S.), pp. 412–415. The Hague, Nijhoff Publishers.Google Scholar
Fujisaki, K., Kamio, T. and Kitaoka, S. (1984) Passage of host serum components including antibodies specific for Theileria sergenti, across the digestive tract of argasid and Ixodid ticks. Ann. trop. Med. Parasitol. 78, 449–450.CrossRefGoogle ScholarPubMed
Galun, R. (1978) Control of livestock pests by interference in their development. UC/AID Pest Management and Relative Environmental Protection Progress Report. Bogota, Colombia.Google Scholar
Irvin, A. D. and Brocklesby, D. W. (1970) Rearing and maintaining Rhipicephalus appendiculatus in the laboratory. J. Ins.Anim. Tech. 21, 106–112.Google Scholar
Kemp, D. H., Agbede, R. I. S., Johnson, L. A. Y. and Gough, J. M. (1986) Immunization of cattle against Boophilus microplus using extracts derived from adult female ticks: Feeding and survival of the parasite on vaccinated cattle. Int. J. Parasitol. 16, 115–120.CrossRefGoogle ScholarPubMed
Minoura, H., Chinzei, Y. and Kitamura, S. (1985) Ornithodoros moubata: host immunoglobulin G in tick haemolymph. Exp. Parasitol. 60, 355–363.CrossRefGoogle Scholar
Ouchterlony, O. (1958) Diffusion-in-gel methods for immunological analysis. In Progress in Allergy (Edited by Kallos, P.), Vol. 5, pp. 1–78. Karger, Bassel.Google Scholar
Roberts, J. A. (1968) Acquisition by the host of resistance to the cattle tick Boophilus microplus (Canestrini). J. Parasitol. 54, 657–662.CrossRefGoogle Scholar
Steelman, C. D. (1976) Effects of external and internal arthropod parasites on domestic livestock production. Annu. Rev. Entomol. 21, 155–178.CrossRefGoogle ScholarPubMed
Tracey-Patte, P. D., Kemp, D. H. and Johnston, L. A. Y. (1987) Boophilus microplus: passage of bovine immunoglobulins and albumin across the gut of cattle ticks feeding on normal or vaccinated cattle. Res. Vet. Sci. 43, 287–290.CrossRefGoogle ScholarPubMed
Voller, A., Bidwell, D. E. and Bartlett, A. (1979) The Enzyme Linked Immunosorbent Assay. Dynatech Laboratories, Alexandria, Virginia.Google Scholar
Wikel, S. K. (1988) Immunological control of haematophagous arthropod vectors: utilization of novel antigens. Vet. Parasitol. 29, 235.CrossRefGoogle Scholar