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Immunization of rabbits with Rhipicephalus appendiculatus antigen–antibody complexes

Published online by Cambridge University Press:  19 September 2011

A. O. Mongi ,
S. Z. Shapiro ,
J. J. Doyle  and
M. P. Cunningham
A. O. Mongi
Affiliation:
The International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
S. Z. Shapiro
Affiliation:
International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya
J. J. Doyle
Affiliation:
International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya
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Abstract

Adult ticks of Rhipicephalus appendiculatus were fed on rabbits immunized with antigen–antibody complexes derived from the immunodiffusion reaction in agarose of tick extracts with antisera from rabbits immunized previously with tick extracts. Ticks feeding on the precipitin complex immunized rabbits displayed delayed attachment, prolonged engorgement and extended periods to drop off the host. While final engorgement weight was not affected, the immunization was observed to produce an adverse effect on egg hatchability. Fewer than 10% eggs from ticks fed on immunized animals hatched live larvae compared to greater than 90% live hatchings from ticks fed on controls. The tick antigens responded to by the immunized rabbits were characterized by crossed immunoelectrophoresis. Antisera from precipitin complex immunized animals precipitated four to five separate antigens from immunizing extracts. One (or more) of these antigens may be the target for the observed deleterious effect on tick reproduction.

Résumé

Les tiques adultes Rhipicephalus appendiculatus sont nourries sur des lapins immunisés avec le complexe antigène–anticorps dérivé de la réaction d'immunodiffusion dans l'agarose des extraits des tiques avec les antisera provenant des lapins préalablement immunisés avec les extraits des tiques.

Les tiques nourries sur des lapins immunisés avec le complexe precipité montrent un attachement labile, un engorgement prolongé et des périodes extensibles pour se détacher de l'animal hôte. Alors que le poids de l'engorgement final n'est pas affecté, l'immunisation observée produit un effet adverse sur l'habileté d'attachement de l'oeuf.

A peine quelque 10% d'oeufs provenant des tiques nourries sur des animaux immunisés lâchent des larves vivantes comparées à plus de 90% des larves lâchées par des tiques nourries sur des animaux contrôlles.

Les antigènes des tiques répondant aux lapins immunisés sont carectérisés par immunoélectrophorèse croisée. Les antisera provenant des animaux immunisés avec le complexe précipitent quatre à cinq antigènes séparables à partir des extraits d'immunisation. Un ou plusieurs de ces antigènes peuvent être des cibles pour l'observation de l'effet de déletion sur la reproduction des tiques.

Keywords

Rhipicephalus appendiculatusantigensantiserumantigen–antibody complexesimmunizationimmunodiffusionimmunoelectrophoresiscrossed-immunoelectrophoresisreproductive potentialRhipicaphalus appendiculatisantigènesantiserumcomplexe antigene–anticorpsimmunizationimmunodiffusionimmunoelectrophorese croisée de s'alimenterpotentiel de reproduction
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 7 , Issue 4 , August 1986 , pp. 471 - 477
Copyright
Copyright © ICIPE 1986

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