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Interaction of Dicladispa armigera (Coleoptera: Chrysomelidae) haemocytes with Beauveria bassiana

Published online by Cambridge University Press:  01 June 2008

Moushumi Phukan ,
L.K. Hazarika ,
Madhumita Barooah ,
K.C. Puzari  and
S. Kalita
Moushumi Phukan
Affiliation:
Department of Entomology, Assam Agricultural University, Jorhat785 013, Assam, India
L.K. Hazarika*
Affiliation:
Department of Entomology, Assam Agricultural University, Jorhat785 013, Assam, India
Madhumita Barooah
Affiliation:
Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat785 013, Assam, India:
K.C. Puzari
Affiliation:
Mycology Research Section, Assam Agricultural University, Jorhat785 013, Assam, India
S. Kalita
Affiliation:
Department of Entomology, Assam Agricultural University, Jorhat785 013, Assam, India
*
*E-mail: lkhazarika@yahoo.com; lkhazarika@aau.ac.in
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Abstract

Morphology of haemocytes of healthy and Beauveria bassiana (Bals.) Vuill.-infected Dicladispa armigera (Olivier) adults was examined in a phase contrast microscope to characterize them into different types. The total haemocyte count (THC) and the differential haemocyte count (DHC) were also estimated. Four types of haemocytes were identified: prohaemocyte, plasmatocyte, granulocyte and spherulocyte. In the healthy adults, the THC varied between 5055 and 5950/mm3, out of which 85% were composed of granulocytes and plasmatocytes. As B. bassiana infection time increased, THC decreased and was associated with a significant concomitant increase in granulocytes and a decrease in prohaemocytes. Granulocytes reacted in various ways, including disintegration of the plasma membrane, formation of fine pseudopod-like cytoplasmic extensions and finally, clumping of cells. Granulocytes and plasmatocytes were the predominant haemocytes involved in all the cell-mediated defence reactions.

Keywords

Dicladispa armigeraBeauveria bassianacellular immunityencapsulation
Type
Research Paper
Information
International Journal of Tropical Insect Science , Volume 28 , Issue 2 , June 2008 , pp. 88 - 97
Copyright
Copyright © ICIPE 2008

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