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Investigations on physiological components of Atherigona soccata larvae and their interaction with sorghum—I. Larval enzymes

Published online by Cambridge University Press:  19 September 2011

Z. T. Dabrowski  and
N. Y. Patel
Z. T. Dabrowski
Affiliation:
International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
N. Y. Patel
Affiliation:
International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
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Abstract

The assumption was made that for a complete understanding of the feeding behaviour and nutrition of Atherigona soccata larvae on various cultivars of sorghum, it was necessary to investigate the relationship between plant chemicals occurring in resistant sorghum plants and the digestive enzymes of larvae. However, at least an introduction to the anatomical organization of the alimentary canal of larvae is needed to understand the digestion and absorption of food taken from resistant and susceptible sorghum lines.

The present preliminary investigation was undertaken to determine qualitatively some of the enzymes present in the gut and salivary glands of the third instar by locating enzymes histochemically on polyacrylamide gels.

Keywords

Atherigona soccataalimentary canalenzymesresistancesalivary glandssorghum shootfly
Type
Research Article
Information
International Journal of Tropical Insect Science , Volume 2 , Special Issue 1-2: The Biology, Ecology and Control of the Sorghum Shootfly, Atherigona soccata Rondani , June 1981 , pp. 73 - 76
Copyright
Copyright © ICIPE 1981

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References

REFERENCES

Blum, A. (1968) Anatomical phenomena in seedlings of sorghum varieties to the sorghum shootfly (Atherigona varia saccata). Crop. Sci. 8, 388–391.CrossRefGoogle Scholar
Davies, B. J. (1964) Disc electrophoresis—II. Method and application to human serum proteins. Ann. N.Y. Acad. Sci. 121, 404–427.CrossRefGoogle Scholar
Diezel, W., Kipperschiläger, G. and Hoffman, E. (1972) An improved procedure for protein staining in poly-acrylamide gels with a new type of Coomassie Brilliant Blue. Analyt. Biochem. 48, 617–620.CrossRefGoogle Scholar
Gabriel, O. (1973) Methods of Enzymology (Ed. by Jacoby, W. B.), Vol. 22, p. 592. Academic Press, New York.Google Scholar
Jotwani, M. G. and Srivastava, K. P. (1970) Studies on sorghum lines resistant against shootfly Atherigona varia seccata (Rond.). Indian J. Ent. 32, 1–3.Google Scholar
Lee, D. and Ryle, A. P. (1967) Pepsinogen D. A fourth proteolyptic zynogen from pig gastric mucosa. Biochem. J. 104, 735.CrossRefGoogle Scholar
Ponnaiya, B. W. X. (1951) Studies in the genus Sorghum: II. The cause of resistance in sorghum to the insect pest Atherigona seccata M. Madras Univ. J. 21, 203–217.Google Scholar
Tingey, N. M. and Singh, S. R. (1980) Environmental factors influencing the magnitude and expression of resistance. In Breeding Plants Resistant to Insects (Ed. by Maxwell, F. G. and Jennings, P. R.), pp. 89–111. John Wiley, New York.Google Scholar
Whitemore, R. and Filbert, L. I. (1974) Haemolymph proteins and lipoproteins in Lepidoptera. A comparative electrophoretic study. Comp. Biochem. Physiol. 47B, 63.Google Scholar
Woodheads, and Bernays, S. E. (1978) The chemical basis of resistance of Sorghum bicolor to attack by Locusta migratoria. Entomologia exp. appi. 24, 133–144.Google Scholar