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Partial larval rearing of Earias vittella (Fabricius) on artificial diet

Published online by Cambridge University Press:  19 September 2011

Gorakh Prasad Gupta
Affiliation:
Division of Entomology, Indian Agricultural Research Institute, New Delhi 110012, India
Gagana Kumar Mahapatro
Affiliation:
Division of Entomology, Indian Agricultural Research Institute, New Delhi 110012, India
Ajanta Chandra
Affiliation:
Division of Entomology, Indian Agricultural Research Institute, New Delhi 110012, India
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Abstract

An artificial diet for rearing the spotted bollworm, Earias vittella (Fabricius) was developed under laboratory conditions (temperature 27 ± 1°C, RH 60 ± 5% and 16:8 h scoto /photophase regime). The diet ingredients consisted of wheatgerm (5 g), seeds of cotton (Gossypium hirsutum L. var. Pusa 8–6) (50 g) and chickpea (Cicer arietinitm L. var. Kabuli)(35 g) as base materials along with agar-agar (15 g), dried yeast powder (8.0 g), methyl-p-hydroxybenzoate (1.0 g), l-ascorbic acid (1.2 g), sorbic acid (1.0 g), cholesterol (0.2 g), streptomycin sulphate (0.2 g), and multivitamin multimineral and vitamin E capsules (one each). After rearing neonates on okra for 3–4 days, the diet supported the growth and development of E. vittella successfully for eight generations. Larval development on this diet was completed in 11.9 ± 0.66 days against 11.64 days on the pest's preferred natural host, okra (Abelmoschus esculentus) Moench. The growth and developmental indices on the artificial diet for the eight generations are reported.

Résumé

Un milieu nutritif artificiel a été mis au point pour l'élevage d'Earias vittella (Fabricius) dans des conditions de laboratoire (T0 = 27 ± 1°C, HR 60 ± 5% et à un régime de 16 h d'obscurité et 8 h d'éclairage. Le milieu nutritif consistait en produits de fond dont: le germe de blé (5 g), les graines de coton, Gossypium hirsutum L. var. Pusa 8–6 (50 g), le pois chiche (Cicer arietinum var. Kabuli) (35 g). A ces produits de fond, on a ajouté de l'agar-agar (15 g), la levure en poudre (8,0 g), le méthyl-p-hydroxybénzoate (1,0 g), L-acid ascorbique (1,2 g), l'acide sorbique (1,0 g), le cholestérol (0,2 g), le sulfate de streptomycine (0,2 g); et des comprimés: multivitamine, multiminéral et vitamine E (un de chaque espèce). Après élevage des chenilles néonates sur le gombo pour 3–4 jours, le milieu artificiel a positivement stimulé la croissance et le développement de E. vittella pour huit générations successives. Le développement de l'état larvaire sur ce milieu durait 11,9 ± 0,66 jours contre 11,64 jours que prend ce développement, une fois l'insecte nourri sur le gombo (Abelmoschus esculenlus) Moench., son hôte naturel favorit. Les indices de croissance et de développement sur milieu artificiel pour les huit générations sont exposés.

Type
Research Articles
Copyright
Copyright © ICIPE 1998

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References

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