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Protein synthesis in the salivary glands of some bugs: An autoradiographic study of (3H) glycine incorporation

Published online by Cambridge University Press:  19 September 2011

P. R. Yadav
P. R. Yadav*
Affiliation:
Department of Zoology, Banaras Hindu University, Varanasi-221 005, India
*
*Silkworm Seed Technology Laboratory, Kodathi, Carmelaram Post Bangalore-560035, India.
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Abstract

Autoradiographic observations using (3H) glycine in the salivary glands of Lethocerus indicus, Laccotrephes rubra and Antilochus coqueberti indicated synthetic activity being performed by the glandular epithelia in all the bugs at short incubation periods. Long incubation periods showed that the site of heavy labelling shifts from outside inwards, with progressive incubation timings, namely from the basal borders and eventually into the lumen. This shifting of the labelling from outside inwards suggests the glandular epithelium is also capable of transporting some of the haemolymph proteins into the gland in all the species and thus indicating heterosynthetic origin of saliva.

Résumé

Des observations autoradographiques utilisant Glycine (3H) au niveau des glandes salivaires de Lethocerus indicus, Laccotrephes rubra et Antilochus coqueberti ont montré une activité de synthèse par l'épithélium glandulaire après une courte période d'incubation. Au cours de longues périodes on remarque au moyen d'un chronométrage progressif du temps d'incubation que le site de concentration du produit de secrétion marqué se déplace de l'extérieur vers l'intérieur surtout de la membrane basale et éventuellement vers la lumière. Ce déplacement de la substance marquée vers l'intérieur suggère que l'épithélium glandulaire est aussi capable de transporter des proteines de l'hémolymphe à la glande dans toutes les espèces indiquant ainsi une origine hétérosynthétique de la salive.

Keywords

Salivary glandsLethocerus indicusLaccotrephes rubraAntilochus coquebertiautoradiography(3H) glycineprotein metabolism
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 13 , Issue 1 , February 1992 , pp. 49 - 58
Copyright
Copyright © ICIPE 1992

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