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Microsatellite markers linked to QTL for resistance to Mal de Río Cuarto disease in Zea mays L.

Published online by Cambridge University Press:  07 December 2004

M. A. DI RENZO ,
N. C. BONAMICO ,
D. G. DÍAZ ,
M. A. IBAÑEZ ,
M. E. FARICELLI ,
M. G. BALZARINI  and
J. C. SALERNO
M. A. DI RENZO
Affiliation:
Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Agencia n°3, 5800 Río Cuarto, Argentina
N. C. BONAMICO
Affiliation:
Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Agencia n°3, 5800 Río Cuarto, Argentina
D. G. DÍAZ
Affiliation:
Instituto de Genética ‘Ewald A. Favret’, Instituto Nacional de Tecnología Agropecuaria, cc 25, 1712 Castelar, Argentina
M. A. IBAÑEZ
Affiliation:
Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Agencia n°3, 5800 Río Cuarto, Argentina
M. E. FARICELLI
Affiliation:
Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Agencia n°3, 5800 Río Cuarto, Argentina
M. G. BALZARINI
Affiliation:
Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba, cc 509, 5000 Córdoba, Argentina
J. C. SALERNO
Affiliation:
Instituto de Genética ‘Ewald A. Favret’, Instituto Nacional de Tecnología Agropecuaria, cc 25, 1712 Castelar, Argentina
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Abstract

‘Mal de Río Cuarto’ (MRC) disease, caused by a member of the family Reoviridae belonging to the genus Fijivirus, is considered to be the most damaging viral disease of maize (Zea mays L.) in Argentina. Resistance to MRC disease is a quantitative trait with moderate heritability ranging from 0·44 to 0·56. The objective of this study was to identify simple sequence repeats (SSR) loci linked to quantitative trait loci (QTL) contributing to MRC disease resistance. Two hundred and twenty-seven F3 derived-lines from a cross between a susceptible inbred line, Mo17, and a partially resistant inbred line, BLS14, were evaluated across four Río Cuarto environments. A disease severity index (DSI) based on disease grades was calculated and used to rate F3 derived-lines for their resistance to MRC disease. A subset of parental F2 plants belonging to susceptible and resistant F3 derived-lines from field assessments was assayed for 180 SSR primer pairs to map resistance genes. Fifty-six maize SSR were employed for the testing of linkage among DNA markers and the mapping of QTL through composite interval mapping. Resistance to MRC disease was affected by two QTL on chromosomes 1 and 8 which showed overdominance and dominant gene action, respectively. A simultaneous fit with these QTL in the joint analyses explained 36·2% of the phenotypic variance. In spite of the fact that relative efficiency of marker-assisted selection (MAS) in comparison to phenotypic selection was close to 1, the mapped QTL could improve the efficiency of efforts in breeding for resistance to MRC disease.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 142 , Issue 3 , June 2004 , pp. 289 - 295
Copyright
© 2004 Cambridge University Press

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