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The use of multiple restriction enzymes in terminal restriction fragment length polymorphism analysis and identification of performance-related caecal bacterial groups in growing broiler chickens

Published online by Cambridge University Press:  08 July 2015

R. RUIZ ,
A. BARROSO-DELJESÚS ,
L. LARA  and
L. A. RUBIO
R. RUIZ
Affiliation:
Fisiología y Bioquímica de la Nutrición Animal (EEZ, CSIC), Profesor Albareda 1, 18008 Granada, Spain
A. BARROSO-DELJESÚS
Affiliation:
Unidad de Genómica, Instituto de Parasitología y Biomedicina López-Neyra, IPBLN-CSIC, PTS Granada, Avda. del Conocimiento s/n, Armilla, 18016 Granada, Spain
L. LARA
Affiliation:
Fisiología y Bioquímica de la Nutrición Animal (EEZ, CSIC), Profesor Albareda 1, 18008 Granada, Spain
L. A. RUBIO*
Affiliation:
Fisiología y Bioquímica de la Nutrición Animal (EEZ, CSIC), Profesor Albareda 1, 18008 Granada, Spain
*
*To whom all correspondence should be addressed. Email: lrubio@eez.csic.es
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Summary

Four restriction enzymes (AluI, HhaI, MspI and RsaI), either individually or in combination, were used in terminal restriction fragment length polymorphism (T-RFLP) analysis to: (i) characterize the chicken intestinal bacterial community; and (ii) tentatively identify intestinal bacterial groups related with increased performance parameters in broiler chickens. Balanced commercial diets free of any feed antibiotics were offered to broilers assigned randomly to one of the five dietary treatments: control (C) (commercial diet with no additive), inulin (I), fructose caramel, propyl propane thiosulphonate (PTS-O)-45 and PTS-O-90. Except for the inulin-supplemented diet, multivariate statistical analysis of T-RFLP profiles based on individual enzymes or their combinations showed that the caecal bacterial community composition was significantly different among diets, particularly between the control and the supplemented diets. Individual RsaI and the combination AluI + RsaI proved to be the most useful to discriminate between dietary treatments. Clostridiaceae 1, Lachnospiraceae, Ruminococcaceae and Micrococcaceae were tentatively identified as those families most likely to be implicated in defining the caecal microbiota composition of growing broiler chickens, and also as those most closely related with differences in productive parameters.

Type
Animal Research Papers
Information
The Journal of Agricultural Science , Volume 153 , Issue 8 , November 2015 , pp. 1491 - 1505
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Copyright
Copyright © Cambridge University Press 2015 

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