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Variation in PLIN2 and its association with milk traits and milk fat composition in dairy cows

Published online by Cambridge University Press:  30 March 2021

Y. H. Li ,
H. Zhou Open the ORCID record for H. Zhou [Opens in a new window] ,
L. Cheng ,
J. Zhao  and
J. G. H. Hickford
Y. H. Li
Affiliation:
Department of Agricultural Sciences, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln7647, New Zealand
H. Zhou
Affiliation:
Department of Agricultural Sciences, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln7647, New Zealand
L. Cheng
Affiliation:
Faculty of Veterinary and Agricultural Sciences, Dookie College, The University of Melbourne, Victoria3647, Australia
J. Zhao
Affiliation:
Department of Agricultural Sciences, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln7647, New Zealand
J. G. H. Hickford*
Affiliation:
Department of Agricultural Sciences, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln7647, New Zealand
*
Author for correspondence: J. G. H. Hickford, E-mail: Jonathan.Hickford@lincoln.ac.nz
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Abstract

The current study investigated associations between variation in the bovine perilipin-2 gene (PLIN2) and milk traits (milk fat content, milk protein content, milk yield and milk fatty acid (FA) component levels) in 409 New Zealand pasture-grazed Holstein-Friesian × Jersey-cross (HF × J-cross or Kiwicross™) cows. Five nucleotide sequence variants were found in three regions of the gene, including c.17C>T in exon 2, c.53A>G in exon 3, c.595+23G>A and c.595+104_595+108del in intron 5, and c.*302T>C in the 3′-untranslated region. The c.*302T>C substitution produces two nucleotide sequence variants (A5 and B5), and this variation was associated with variation in milk protein content and milkfat composition for C10:0, C11:0, C12:0, C13:0 and C16:0 FA and medium-chain fatty acid (MCFA) and long-chain fatty acid (LCFA) groups. After correcting for the effect of variation in the diacylglycerol acyl-CoA acyltransferase 1 gene (DGAT1) that results in the amino acid substitution p.K232A, variation in the FA binding protein 4 gene (FABP4) and variation in the stearoyl-CoA desaturase (Δ-9-desaturase) gene (SCD) that results in the amino acids substitution p.A293V, significant differences between A5A5 and B5B5 cows were found for C10:0, C11:0, C12:0, C13:0, C16:0, and the MCFA, LCFA, total saturated FA and C10:1 index groups. This suggests that nucleotide sequence variation in PLIN2 may be affecting milk FA component levels.

Keywords

Genetic variationmilk fatty acidPLIN2
Type
Animal Research Paper
Information
The Journal of Agricultural Science , Volume 158 , Issue 8-9 , November 2020 , pp. 774 - 780
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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References

Bionaz, M and Loor, J (2008) Gene networks driving bovine milk fat synthesis during the lactation cycle. BMC Genomics 9, 366.CrossRefGoogle ScholarPubMed
Brasaemle, DL, Wolins, NE, Londos, C, Barber, T, Blanchette-Mackie, EJ, Serrero, G and Wolins, NE (1997) Adipose differentiation-related protein was an ubiquitously expressed lipid storage droplet-associated protein. Journal of Lipid Research 38, 2249–2263.CrossRefGoogle ScholarPubMed
Byun, SO, Fang, Q, Zhou, HT and Hickford, JGH (2009) An effective method for silver-staining DNA in large numbers of polyacrylamide gels. Analytical Biochemistry 385, 174–175.CrossRefGoogle ScholarPubMed
Cheong, HS, Yoon, DH, Bae, JS, Kim, LH, Kim, EM, Kim, JO, Hong, J, Kim, N and Shin, HD (2009) ADFP promoter polymorphism associated with marbling score in Korean cattle. BMB Reports 42, 529–534.CrossRefGoogle ScholarPubMed
Chong, BM, Reigan, P, Mayle-Combs, KD, Orlicky, DJ and McManaman, JL (2011) Determinants of adipophilin function in milk lipid formation and secretion. Trends in Endocrinology & Metabolism, Review Special issue: Lipid Droplet Metabolism 22, 211–217.CrossRefGoogle ScholarPubMed
Gong, H, Zhou, H and Hickford, JGH (2011) Diversity of the glycine/tyrosine-rich keratin-associated protein 6 gene (KAP6) family in sheep. Molecular Biology Reports 38, 31–35.CrossRefGoogle ScholarPubMed
Grisart, B, Coppieters, W, Farnir, F, Karim, L, Ford, C, Berzi, P, Cambisano, N, Mni, M, Reid, S, Simon, P, Spelman, R, Georges, M and Snell, R (2002) Positional candidate cloning of a QTL in dairy cattle: identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition. Genome Research 12, 222–231.CrossRefGoogle ScholarPubMed
Heid, HW, Schnolzer, M and Keenan, TW (1996) Adipocyte differentiation related protein is secreted into milk as a constituent of milk lipid globule membrane. Biochemical Journal 320, 1025–1030.CrossRefGoogle ScholarPubMed
Imai, Y, Varela, GM, Jackson, MB, Graham, MJ, Crooke, RM and Ahima, RS (2007) Reduction of hepatosteatosis and lipid levels by an adipose differentiation-related protein antisense oligonucleotide. Gastroenterology 132, 1947–1954.CrossRefGoogle ScholarPubMed
Imamura, M, Inoguchi, T, Kobayashi, K, Nakashima, N, Nawata, H, Ikuyama, S and Taniguchi, S (2002) ADRP stimulates lipid accumulation and lipid droplet formation in murine fibroblasts. American Journal of Physiology – Endocrinology and Metabolism 283, E775–E783.CrossRefGoogle ScholarPubMed
Ju, Z, Wang, C, Wang, X, Yang, C, Zhang, Y, Sun, Y, Jiang, Q, Li, R, Li, J, Zhong, J and Huang, J (2018) The effect of the SNP g.18475 A>G in the 3′ UTR of NCF4 on mastitis susceptibility in dairy cattle. Cell Stress & Chaperones 23, 385–391.CrossRefGoogle ScholarPubMed
Khatib, H, Leonard, SD, Schutzkus, V, Luo, W and Chang, YM (2006) Association of the OLR1 gene with milk composition in Holstein dairy cattle. Journal of Dairy Science 89, 1753–1760.CrossRefGoogle ScholarPubMed
Li, L, Huang, J, Zhang, X, Ju, Z, Qi, C, Zhang, Y, Li, Q, Wang, C, Miao, W, Zhong, J, Hou, M and Hang, S (2012) One SNP in the 3′-UTR of HMGB1 gene affects the binding of target bta-miR-223 and was involved in mastitis in dairy cattle. Immunogenetics 64, 817–824.CrossRefGoogle ScholarPubMed
Li, Z, Guo, W, Tian, Y, Han, R, Sun, Y, Xue, J, Lan, X and Chen, H (2014) Characterisation of the genetic effects of the ADFP gene and its association with production traits in dairy goats. Gene 538, 244–250.CrossRefGoogle ScholarPubMed
Li, YH, Zhou, HT, Cheng, L, Hodge, M, Tung, R, Zhao, J, Edwards, GR and Hickford, JGH (2020 a) Effects of FABP4 variation on milk fatty-acid composition in dairy cattle grazed on pasture in late lactation. Journal of Dairy Research 87, 32–36.CrossRefGoogle ScholarPubMed
Li, YH, Zhou, HT, Cheng, L, Edwards, GR and Hickford, JGH (2020 b) Variation in the bovine SCD1 gene affects milk fatty-acid composition in outdoor pasture-grazed dairy cattle. Archives Animal Breeding 63, 355–366.CrossRefGoogle Scholar
Li, YH, Zhou, HT, Cheng, L, Edwards, GR and Hickford, JGH (2021) Effect of DGAT1 variant (K232A) on milk traits and milk fat composition in outdoor pasture-grazed dairy cattle. New Zealand Journal of Agricultural Research 64, 101–103.CrossRefGoogle Scholar
Listenberger, L, Ostermeyer-Fay, AG, Goldberg, E, Brown, W and Brown, D (2007) Adipocyte differentiation-related protein reduces the lipid droplet association of adipose triglyceride lipase and slows triacylglycerol turnover. Journal of Lipid Research 48, 2751–2761.CrossRefGoogle ScholarPubMed
Lu, J, Argov-Argaman, N, Anggrek, J, Boeren, S, van Hooijdonk, T, Vervoort, J and Hettinga, KA (2016) The protein and lipid composition of the membrane of milk fat globules depends on their size. Journal of Dairy Science 99, 4726–4738.CrossRefGoogle ScholarPubMed
Magra, AL, Mertz, PS, Torday, JS and Londos, C (2006) Role of adipose differentiation-related protein in lung surfactant production: a reassessment. Journal of Lipid Research 47, 2367–2373.CrossRefGoogle ScholarPubMed
McManaman, J, Russell, T, Schaack, J, Orlicky, D and Robenek, H (2007) Molecular determinants of milk lipid secretion. Journal of Mammary Gland Biology and Neoplasia 12, 259–268.CrossRefGoogle ScholarPubMed
Ogorevc, J, Kunej, T, Razpet, A and Dovc, P (2009) Database of cattle candidate genes and genetic markers for milk production and mastitis. Animal Genetics 40, 832–851.CrossRefGoogle ScholarPubMed
Prats, C, Donsmark, M, Qvortrup, K, Londos, C, Sztalryd, C, Holm, C, Galbo, H and Ploug, T (2006) Decrease in intramuscular lipid droplets and translocation of HSL in response to muscle contraction and epinephrine. Journal of Lipid Research 47, 2392–2399.CrossRefGoogle ScholarPubMed
Reinhardt, TA and Lippolis, JD (2006) Bovine milk fat globule membrane proteome. Journal of Dairy Research 73, 406–416.CrossRefGoogle ScholarPubMed
Russell, TD, Palmer, CA, Orlicky, DJ, Fischer, A, Rudolph, MC, Neville, MC and McManaman, JL (2007) Cytoplasmic lipid droplet accumulation in developing mammary epithelial cells: roles of adipophilin and lipid metabolism. Journal of Lipid Research 48, 1463–1475.CrossRefGoogle ScholarPubMed
Thering, BJ, Graugnard, DE, Piantoni, P and Loor, JJ (2009) Adipose tissue lipogenic gene networks due to lipid feeding and milk fat depression in lactating cows. Journal of Dairy Science 92, 4290–4300.CrossRefGoogle ScholarPubMed
Weikard, R, Kühn, C, Goldammer, T, Freyer, G and Schwerin, M (2005) The bovine PPARGC1A gene: molecular characterization and association of an SNP with variation of milk fat synthesis. Physiological Genomics 21, 1–13.CrossRefGoogle ScholarPubMed
Zhao, X, Liu, Y, Jiang, X, Du, H and Zhu, Q (2009) Association of polymorphisms of chicken adipose differentiation-related protein gene with carcass traits. Journal of Poultry Science 46, 87–94.CrossRefGoogle Scholar
Zhou, H, Hickford, JGH and Fang, Q (2006) A two-step procedure for extracting genomic DNA from dried blood spots on filter paper for polymerase chain reaction amplification. Analytical Biochemistry 354, 159–161.CrossRefGoogle ScholarPubMed
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