Hostname: page-component-745bb68f8f-cphqk Total loading time: 0 Render date: 2025-01-14T00:18:38.508Z Has data issue: false hasContentIssue false
  • English
  • Français

Casein composition and differential translational efficiency of casein transcripts in donkey's milk

Published online by Cambridge University Press:  30 April 2019

Gianfranco Cosenza ,
Rosalba Mauriello ,
Giuseppina Garro ,
Barbara Auzino ,
Marco Iannaccone ,
Angela Costanzo ,
Lina Chianese  and
Alfredo Pauciullo
Gianfranco Cosenza*
Affiliation:
Department of Agricultural Sciences, University of Naples Federico II, Portici (Naples), Italy
Rosalba Mauriello
Affiliation:
Department of Agricultural Sciences, University of Naples Federico II, Portici (Naples), Italy
Giuseppina Garro
Affiliation:
Department of Agricultural Sciences, University of Naples Federico II, Portici (Naples), Italy
Barbara Auzino
Affiliation:
Department of Veterinary Sciences, University of Pisa, Pisa, Italy
Marco Iannaccone
Affiliation:
Department of Agricultural Sciences, University of Naples Federico II, Portici (Naples), Italy
Angela Costanzo
Affiliation:
Department of Agricultural Sciences, University of Naples Federico II, Portici (Naples), Italy
Lina Chianese
Affiliation:
Department of Agricultural Sciences, University of Naples Federico II, Portici (Naples), Italy
Alfredo Pauciullo
Affiliation:
Department of Agricultural, Forest and Food Science, University of Torino, Grugliasco (TO), Italy
*
Author for correspondence: Gianfranco Cosenza, Email: giacosen@unina.it
Get access Rights & Permissions [Opens in a new window]

Abstract

The amount of the four caseins (αs1, αs2, β and κ-CN) in donkey milk was evaluated by Urea-PAGE analysis at pH 8.6, followed by immuno-detection with polyclonal antibodies, coupled to densitometric analysis. The results showed the percentage of each casein in decreasing order: β (54.28) > αs1 (35.59) > αs2 (7.19) > κ-CN (2.79). The mRNA quantification of donkey casein transcripts, carried out by RT-qPCR, showed that the average percentage of corresponding gene transcripts (CSN2, CSN1S1, CSN1S2 I and CSN3) was 70.85, 6.28, 14.23 and 8.65, respectively. The observed translation efficiency, assessed as percentage of single milk casein fraction out of single percentage of transcript, was 0.76, 5.66, 0.50 and 0.32, respectively. The analysis of the sequences flanking the start codon, the codon usage frequencies and the coding sequence length might explain, at least in part, the differential transcriptional and translational rate observed among the casein transcripts.

Keywords

CaseindonkeymRNAquantification
Type
Research Article
Information
Journal of Dairy Research , Volume 86 , Issue 2 , May 2019 , pp. 201 - 207
Copyright
Copyright © Hannah Dairy Research Foundation 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

†

These authors contributed equally to this work.

References

Aschaffenburg, R and Drewry, J (1959) New procedure for the routine determination of the various non-casein proteins of milk. In London: XVth International Dairy Congress. vol. 3, pp. 1631–1637.Google Scholar
Afshar-Kharghan, V, Li, CQ, Khoshnevis-Asl, M and Lopez, JA (1999) Kozak sequence polymorphism of the glycoprotein (GP) Iba gene is a major determinant of the plasma membrane levels of the platelet GP Ib-IX-V complex. Blood 94, 186–191.Google Scholar
Bai, WL, Yin, RH, Jiang, WQ, Ajayi, OO, Zhao, SJ, Lu, GB, Zhao, ZH and Imumorin, IG (2013) Molecular analysis of alpha(s1)-, beta-, alpha(s2)- and kappa-casein transcripts reveals differential translational efficiency in yak lactating mammary gland. Livestock Science 152, 74–78.Google Scholar
Bevilacqua, C, Martin, P, Candalh, C, Fauquant, J, Piot, M, Roucayrol, AM, Pilla, F and Heyman, M (2001) Goats' milk of defective alpha(s1)-casein genotype decreases intestinal and systemic sensitization to beta-lactoglobulin in Guinea pigs. Journal of Dairy Research 68, 217–227.Google Scholar
Bevilacqua, C, Helbling, JC, Miranda, G and Martin, P (2006) Translational efficiency of casein transcripts in the mammary tissue of lactating ruminants. Reproduction Nutrition Development 5, 567–578.Google Scholar
Businco, L, Giampietro, PG, Lucenti, P, Lucaroni, F, Pini, C, Di Felice, G, Lacovacci, P, Curadi, C and Orlandi, M (2000) Allergenicity of mare's milk in children with cow's milk allergy. Journal of Allergy and Clinical Immunology 105, 1031–1034.Google Scholar
Chianese, L, Quarto, M, Pizzolongo, F, Calabrese, MG, Caira, S, Mauriello, R, De Pascale, S and Addeo, F (2009) Occurrence of genetic polymorphism at the αs1-casein locus in Mediterranean water buffalo milk. International Dairy Journal 19, 181–189.Google Scholar
Chianese, L, Calabrese, MG, Ferranti, P, Mauriello, R, Garro, G, De Simone, C, Quarto, M, Addeo, F, Cosenza, G and Ramunno, L (2010) Proteomic characterization of donkey milk ‘caseome’. Journal of Chromatography A1217, 4834–4840.Google Scholar
Cosenza, G, Pauciullo, A, Annunziata, AL, Rando, A, Chianese, L, Marletta, D, Iannolino, G, Nicodemo, D, Di Berardino, D and Ramunno, L (2010) Identification and characterization of the donkey CSN1S2 I and II cDNAs and polymorphisms detection. Italian Journal of Animal Science 9(e40), 206–211.Google Scholar
Cosenza, G, Pauciullo, A, Coletta, A, Di Francia, A, Feligini, M, Gallo, D, Di Berardino, D and Ramunno, L (2011) Translational efficiency of casein transcripts in Mediterranean river buffalo. Journal of Dairy Science 94, 5691–5694.Google Scholar
Cosenza, G, Pauciullo, A, Gallo, D, Colimoro, L, D'avino, A, Mancusi, A and Ramunno, L (2008) Genotyping at the CSN1S1 locus by PCR-RFLP and AS-PCR in a Neapolitan Goat Population. Small Ruminant Research 74, 84–90.Google Scholar
Criscione, A, Cunsolo, V, Bordonaro, S, Guastella, AM, Saletti, R, Zuccaro, A, D'Urso, G and Marletta, D (2009) Donkeys' milk protein fraction investigated by electrophoretic methods and mass spectrometric analysis. International Dairy Journal 19, 190–197.Google Scholar
Cunsolo, V, Cairone, E, Fontanini, D, Criscione, A, Muccilli, V, Saletti, R and Foti, S (2009 a) Sequence determination of αs1-casein isoforms from donkey by mass spectrometric methods. Journal of Mass Spectrometry 44, 1742–1753.Google Scholar
Cunsolo, V, Cairone, E, Saletti, R, Muccilli, V and Foti, S (2009 b) Sequence and phosphorylation level determination of two donkey beta-caseins by mass spectrometry. Rapid Communication in Mass Spectrometry 23, 1907–1916.Google Scholar
Cunsolo, V, Saletti, R, Muccilli, V, Gallina, S, Di Francesco, A and Foti, S (2017) Proteins and bioactive peptides from donkey milk: the molecular basis for its reduced allergenic properties. Food Research International 99, 41–47.Google Scholar
D'Alessandro, AG, Rosanna De Petro, R, Claps, S, Pizzillo, M and Martemucci, G (2009) Yield and quality of milk and udder health in Martina Franca ass: effects of daily interval and time of machine milking. Italian Journal of Animal Science 8, 697–699.Google Scholar
De Angioletti, M, Lacerra, G, Sabato, V and Carestia, C (2004) β + 45 G —> C: a novel silent β-thalassaemia mutation, the first in the Kozak sequence. British Journal of Haematology 124, 224–231.+C:+a+novel+silent+β-thalassaemia+mutation,+the+first+in+the+Kozak+sequence.+British+Journal+of+Haematology+124,+224–231.>Google Scholar
Djuranovic, S, Nahvi, A and Green, R (2012) miRNA-mediated gene silencing by translational repression followed by mRNA deadenylation and decay. Science 336, 237–240.Google Scholar
Giorgis, V, Rolla, G, Raie, A, Geuna, M, Boita, M, Lamberti, C, Nebbia, S, Giribaldi, M, Giuffrida, M and Brussino, L (2018) A case of work-related donkey milk allergy. Journal of Investigational Allergology and Clinical Immunology 28, 197–199.Google Scholar
Girardet, JM, Miclo, L, Florent, S, Mollè, D and Gaillard, JL (2006) Determination of the phosphorylation level and deamination susceptibility of equine β-casein. Proteomics 6, 3707–3717.Google Scholar
Guo, HY, Pang, K, Zhang, XY, Zhao, L, Chen, SW, Dong, ML and Ren, FZ (2007) Composition, physiochemical properties, nitrogen fraction distribution, and amino acid profile of donkey milk. Journal of Dairy Science 90, 1635–1643.Google Scholar
Jacobson, EM, Concepcion, E, Oashi, T and Tomer, Y (2005) A Graves' disease-associated Kozak sequence single-nucleotide polymorphism enhances the efficiency of CD40 gene translation: a case for translational pathophysiology. Endocrinology 146, 2684–2691.Google Scholar
Hobor, S, Kunej, T and Dovc, P (2008) Polymorphisms in the kappa casein (CSN3) gene in horse and comparative analysis of its promoter and coding region. Animal Genetics 39, 520–530.Google Scholar
Huang, T, Wan, S, Xu, Z, Zheng, Y, Feng, KY, Li, HP, Kong, X and Cai, YD (2011) Analysis and prediction of translation rate based on sequence and functional features of the mRNA. PLoS One 6, e16036.Google Scholar
IDF (1993) Milk. Determination of Nitrogen Content. Standard Method 20B. Brussels, Belgium: International Dairy Federation.Google Scholar
Kappeler, S, Farah, Z and Puhan, Z (1998) Sequence analysis of Camelus dromedarius milk caseins. Journal of Dairy Research 65, 209–222.Google Scholar
Kim, JJ, Yu, J, Bag, J, Bakovic, M and Cant, JP (2015) Translation attenuation via 3′ terminal codon usage in bovine CSN1S2 is responsible for the difference in αs2- and β-casein profile in milk. RNA Biology 12, 354–367.Google Scholar
Kozak, M (1984) Point mutations close to the AUG initiator codon affect the efficiency of translation of rat preproinsulin in vivo. Nature 308, 241–246.Google Scholar
Kozak, M (1987) Analysis of 50-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Research 15, 8125–8148.Google Scholar
Kozak, M (1994) Features in the 5′ non-coding sequences of rabbit α and β-globin mRNAs that affect translational efficiency. Journal of Molecular Biology 235, 95–110.Google Scholar
Lonnerdal, B (1994) Nutritional importance of Non-protein nitrogen protein. Metabolism during infancy. In Raiha, Niels CR (ed), Nestle Nutrition Workshop Series. New York: Ed. Nestec Ltd., Vevey/Raven Press, Ltd., p. 33.Google Scholar
Malacarne, M, Martuzzi, F, Summer, A and Mariani, P (2002) Protein and fat composition of mare's milk: some nutritional remarks with reference to human and cow's milk. International Dairy Journal 12, 869–877.Google Scholar
Martin, P, Ferranti, P, Leroux, C and Addeo, F (2003) Non-bovine caseins: quantitative variability and molecular diversity. In Fox, PF and McSweeney, PLH (eds), Advanced Dairy Chemistry Volume 1: Proteins, 3rd edn. Kluwer Academic/Plenum Publishers.Google Scholar
Mateos, A, Miclo, L, Molle, D, Dary, A, Girardet, JM and Gaillard, JL (2009) Equine αs1- casein: characterization of alternative splicing isoforms and determination of phosphorylation levels. Journal of Dairy Science 92, 3604–3615.Google Scholar
Miranda, G, Mahé, MF, Leroux, C and Martin, P (2004) Proteomic tools to characterize the protein fraction of Equidae milk. Proteomics 4, 2496–2509.Google Scholar
Monti, G, Viola, S, Baro, C, Cresi, F, Tovo, PA, Moro, G and Bertino, E (2012) Tolerability of donkey's milk in 92 highly-problematic cow's milk allergic children. Journal of Biological Regulators and Homeostatic Agents 26(Suppl 3), 75–82.Google Scholar
Ochirkhuyag, B, Chobert, JM, Dalgalarrondo, M and Haertlé, T (2000) Characterization of mare caseins. Identification of αs1- and αs2-caseins. Lait 80, 223–235.Google Scholar
Pineda, B, Laporta, P, Hermenegildo, C, Cano, A and García-Pérez, MA (2008) A C > T polymorphism located at position −1 of the Kozak sequence of CD40 gene is associated with low bone mass in Spanish postmenopausal women. Osteoporosis International 19, 1147–1152.+T+polymorphism+located+at+position+−1+of+the+Kozak+sequence+of+CD40+gene+is+associated+with+low+bone+mass+in+Spanish+postmenopausal+women.+Osteoporosis+International+19,+1147–1152.>Google Scholar
Roncada, P, Piras, C, Soggiu, A, Turk, R, Urbani, A and Bonizzi, L (2012) Farm animal milk proteomics. Journal of Proteomics 75, 4259–4274.Google Scholar
Salimei, E, Fantuz, F, Coppola, R, Chiofalo, B, Polidori, P and Varisco, G (2004) Composition and characteristics of asses' milk. Animal Research 53, 67–78.Google Scholar
Sodeland, M, Grove, H, Kent, M, Taylor, S, Svendsen, M, Hayes, BJ and Lien, S (2011) Molecular characterization of a long range haplotype affecting protein yield and mastitis susceptibility in Norwegian Red cattle. BMC Genetics 12, 70.Google Scholar
Usuki, F and Maruyama, K (2000) Ataxia caused by mutations in the alpha-tocopherol transfer protein gene. Journal of Neurology, Neurosurgery and Psychiatry 69, 254–256.Google Scholar
Valleriani, A, Zhang, G, Nagar, A, Ignatova, Z and Lipowsky, R (2011) Length-dependent translation of messenger RNA by ribosomes. Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics 83(4 Pt 1), 042903.Google Scholar
Supplementary material: PDF

Cosenza et al. supplementary material

Cosenza et al. supplementary material 1

Download Cosenza et al. supplementary material(PDF)
PDF 178.4 KB