Hostname: page-component-78c5997874-mlc7c Total loading time: 0 Render date: 2024-11-10T13:56:24.240Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of diet supplementation with sunflower oil on milk production, fatty acid profile and ruminal fermentation in lactating dairy ewes

Published online by Cambridge University Press:  14 August 2008

Gonzalo Hervás ,
Pilar Luna ,
Ángel R. Mantecón ,
Natalia Castañares ,
Miguel Angel de la Fuente ,
Manuela Juárez  and
Pilar Frutos
Gonzalo Hervás
Affiliation:
Estación Agrícola Experimental (CSIC) Finca Marzanas, 24346 Grulleros, León, Spain
Pilar Luna
Affiliation:
Instituto del Frío (CSIC)José Antonio Novais 10, 28040 Madrid, Spain
Ángel R. Mantecón
Affiliation:
Estación Agrícola Experimental (CSIC) Finca Marzanas, 24346 Grulleros, León, Spain
Natalia Castañares
Affiliation:
Estación Agrícola Experimental (CSIC) Finca Marzanas, 24346 Grulleros, León, Spain
Miguel Angel de la Fuente*
Affiliation:
Instituto del Frío (CSIC)José Antonio Novais 10, 28040 Madrid, Spain
Manuela Juárez
Affiliation:
Instituto del Frío (CSIC)José Antonio Novais 10, 28040 Madrid, Spain
Pilar Frutos
Affiliation:
Estación Agrícola Experimental (CSIC) Finca Marzanas, 24346 Grulleros, León, Spain
*
*For correspondence; e-mail: mafl@if.csic.es
Get access Rights & Permissions [Opens in a new window]

Abstract

The aim of this research was to enhance the nutritional quality of ewe milk fat by increasing potentially healthy fatty acids (FA) through diet supplementation with unprotected oil rich in linoleic acid, and without detrimental effects on animal performance. Twenty-four ewes were assigned to two high concentrate diets, control or supplemented with 6% sunflower oil (SO), for 4 weeks. No differences between treatments were found in milk production and dry matter intake. Although the SO diet increased milk fat percentage and tended to reduce milk protein concentration, it did not affect milk fat, protein or total solid yield. Most of the modifications in milk FA composition were addressed toward a potentially healthier profile: a decrease in C12:0 to C16:0 and a remarkable increase in the contents of cis-9 trans-11 C18:2 (from 0·94 to 3·60 g/100 g total FA) and trans-11 C18:1 (from 2·23 to 8·61 g/100 g total FA). Furthermore, the levels reached were maintained throughout the period monitored. However, the SO diet increased other trans C18:1 isomer percentages, too. The lack of differences between treatments in the in vitro ruminal fermentation parameters, studied with batch cultures of rumen microorganisms, would indicate no negative effects on ruminal fermentation.

Keywords

Conjugated linoleic acidfatty acidmilksheepvaccenic acid
Type
Research Article
Information
Journal of Dairy Research , Volume 75 , Issue 4 , November 2008 , pp. 399 - 405
Copyright
Copyright © Proprietors of Journal of Dairy Research 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

AOAC 2006 Official methods of analysis of the Association of Official Agricultural Chemists. 18th Edn. (1st revision). AOAC International, Gaithersburg, MD, USAGoogle Scholar
Blümmel, M, Makkar, HPS & Becker, K 1997 In vitro gas production: a technique revisited. Journal of Animal Physiology and Animal Nutrition 77 24–34CrossRefGoogle Scholar
Caja, G & Bocquier, F 2000 Effects of nutrition on the composition of sheep milk. Cahier Options Méditerranees 52 59–74Google Scholar
Casals, R, Caja, G, Pol, MV, Such, X, Albanell, E, Gargouri, A & Casellas, J 2006 Response of lactating ewes to various levels of dietary calcium soaps of fatty acids. Animal Feed Science and Technology 131 312–332CrossRefGoogle Scholar
Chilliard, Y, Ferlay, A, Rouel, J & Lamberet, G 2003 A review of nutritional and physiological factors affecting goat milk lipid synthesis and lipolysis. Journal of Dairy Science 86 1751–1770CrossRefGoogle ScholarPubMed
Chilliard, Y & Ferlay, A 2004 Dietary lipids and forages interactions on cow and goat milk fatty acid composition and sensory properties. Reproduction Nutrition and Development 44 467–492CrossRefGoogle ScholarPubMed
Collomb, M, Sieber, R & Bütikofer, U 2004 CLA isomers in milk fat from cows fed diets with high levels of unsaturated fatty acids. Lipids 39 355–364CrossRefGoogle ScholarPubMed
Doreau, M & Ferlay, A 1995 Effect of dietary lipids on nitrogen metabolism in the rumen: a review. Livestock Production Science 43 97–110CrossRefGoogle Scholar
Doreau, M, Demeyer, DI & Van Nevel, CJ 1997 Transformations and effects of unsaturated fatty acids in the rumen. Consequences on milk fat secretion. In: Milk Composition, Production and Biotechnology pp. 73–92 (Eds Welch, RAS, Burns, DJW, Davis, SR, Popay, AI & Prosser, CG). Wallingford: CAB InternationalGoogle Scholar
Frutos, P, Hervás, G, Giráldez, FJ & Mantecón, AR 2004 An in vitro study on the ability of polyethylene glycol to inhibit the effect of quebracho tannins and tannic acid on rumen fermentation in sheep, goats, cows and deer. Australian Journal of Agricultural Research 55 1125–1132CrossRefGoogle Scholar
Gargouri, A, Caja, G, Casals, R & Mezghani, I 2006 Lactational evaluation of effects of calcium soap of fatty acids on dairy ewes. Small Ruminant Research 66 1–10CrossRefGoogle Scholar
Goering, MK & Van Soest, PJ 1970 Forage fiber analysis (apparatus, reagents, procedures and some applications). Agriculture Handbook, No. 379. Agricultural Research Service, USDA. Washington, USAGoogle Scholar
Gómez-Cortés, P, Frutos, P, Mantecón, AR, Juárez, M, De la Fuente, MA & Hervás, G 2008 Milk production, conjugated linoleic acid content, and in vitro ruminal fermentation in response to high levels of soybean oil in dairy ewe diet. Journal of Dairy Science 91 1560–1569CrossRefGoogle ScholarPubMed
Harfoot, CG & Hazlewood, GP 1997 Lipid Metabolism in the rumen. In: The Rumen microbial ecosystem, pp. 382–426 (Ed. Hobson, PM). New York: ElsevierGoogle Scholar
Hervás, G, Frutos, P, Giráldez, FJ, Mora, MJ, Fernández, B & Mantecón, AR 2005 Effect of preservation on fermentative activity of rumen fluid inoculum for in vitro gas production techniques. Animal Feed Science and Technology 123–124 107–118.CrossRefGoogle Scholar
ISO-IDF 2002 Milk fat-Preparation of fatty acid methyl esters. International Standard ISO 15884-IDF 182:2002Google Scholar
Jalč, D, Kišidayova, S & Nerud, F 2007 Effect of plant oils and organic acids on rumen fermentation in vitro. Folia Microbiology 47 171–177CrossRefGoogle Scholar
Jarrige, R 1988 Alimentation des Bovins, Ovins et Caprins. Service des publications de I′INRA. Versailles, FranceGoogle Scholar
Jenkins, TC 1993 Lipid metabolism in the rumen. Journal of Dairy Science 76 3851–3863CrossRefGoogle ScholarPubMed
Lock, AL, Teles, BM, Perfield, JW II, Bauman, DE & Sinclair, LA 2006 A conjugated linoleic acid supplement containing trans-10 cis-12 reduces milk fat synthesis in lactating ewe. Journal of Dairy Science 89 1525–1532CrossRefGoogle Scholar
Luna, P, Juárez, M & De la Fuente, MA 2005 Validation of a rapid milk fat separation method to determine the fatty acid profile by gas chromatography. Journal of Dairy Science 88 3377–3381CrossRefGoogle ScholarPubMed
Mauricio, RM, Mould, FL, Dhanoa, MS, Owen, E, Channa, KS & Theodorou, MK 1999 A semi-automated in vitro gas production technique for ruminant feedstuff evaluation. Animal Feed Science and Technology 79 321–330CrossRefGoogle Scholar
Mele, M, Buccioni, A, Petacchi, F, Serra, A, Banni, S, Antongiovanni, M & Secchiari, P 2006 Effect of forage/concentrate ratio and soybean oil supplementation on milk yield, and composition from Sarda ewes. Animal Research 55 273–285CrossRefGoogle Scholar
Palmquist, DL & Jenkins, TC 1980 Fat in lactation rations: Review. Journal of Dairy Science 63 1–14CrossRefGoogle ScholarPubMed
Palmquist, DL, Lock, AL, Shingfield, KJ & Bauman, DE 2005 Biosynthesis of conjugated linoleic acid in ruminants and humans. Advances in Food Nutrition Research 50 179–217CrossRefGoogle ScholarPubMed
Perfield, JW II, Lock, AL, Griinari, JM, Sæbo, A, Delmonte, P, Dwyer, DA & Bauman, DE 2007 Trans-9, Cis-11 conjugated linoleic acid reduces milk fat synthesis in lactating dairy cows. Journal of Dairy Science 90 2211–2218CrossRefGoogle ScholarPubMed
Reynolds, CK, Cannon, VL & Loerch, SC 2006 Effects of forage source and supplementation with soybean and marine algal oil on milk fatty acid composition of ewes. Animal Feed Science and Technology 131 333–357CrossRefGoogle Scholar
SAS 1999 SAS OnlineDoc®, Version 8, SAS Institute Inc., Cary, NC, USAGoogle Scholar
Schmidt, GH 1971 Biology of Lactation. San Francisco: W.H. Freeman and CompanyGoogle Scholar
Shingfield, KJ, Reynolds, CK, Hervás, G, Griinari, JM, Grandison, AS & Beever, DE 2006 Examination of the persistency of milk fatty acid composition responses to fish oil and sunflower oil in the diet of dairy cows. Journal of Dairy Science 89 714–732CrossRefGoogle ScholarPubMed
Sinclair, LA, Cooper, SL, Chikunya, S, Wilkinson, RG, Hallett, KG, Enser, M & Wood, JD 2005 Biohydrogenation of n-3 polyunsaturated fatty acids in the rumen and their effects on microbial metabolism and plasma fatty acid concentrations in sheep. Animal Science 81 239–248CrossRefGoogle Scholar
Sinclair, LA, Lock, AL, Early, R & Bauman, DE 2007 Effects of trans-10, cis-12 conjugated linoleic acid on ovine milk fat synthesis and cheese properties. Journal of Dairy Science 90 3326–3335CrossRefGoogle ScholarPubMed
Szumacher-Strabel, M, Martin, SA, Potkanski, A, Cieslak, A & Kowalczyk, J 2004 Changes in fermentation processes as the effect of vegetable oil supplementation in in vitro studies. Journal of Animal Feed Science 13 (Suppl. 1) 215–218CrossRefGoogle Scholar
Theodorou, MKB, Williams, A, Dhanoa, MS, McAllan, AB & France, J 1994 A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal Feed Science and Technology 48 185–197CrossRefGoogle Scholar
Van Soest, PJ, Robertson, JB & Lewis, A 1991 Methods for dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74 3583–3597CrossRefGoogle Scholar
Wang, Z & Goonewardene, LA 2004 The use of MIXED models in the analysis of animal experiments with repeated measures data. Canadian Journal of Animal Science 84 1–11CrossRefGoogle Scholar