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Effect of feeding linseed oil in diets differing in forage to concentrate ratio: 1. Production performance and milk fat content of biohydrogenation intermediates of α-linolenic acid

Published online by Cambridge University Press:  16 January 2014

Leacady Saliba ,
Rachel Gervais ,
Yolaine Lebeuf  and
P. Yvan Chouinard
Leacady Saliba
Affiliation:
Département des sciences animales, Université Laval, Québec, Québec G1V 0A6, Canada
Rachel Gervais
Affiliation:
Département des sciences animales, Université Laval, Québec, Québec G1V 0A6, Canada
Yolaine Lebeuf
Affiliation:
Département des sciences animales, Université Laval, Québec, Québec G1V 0A6, Canada
P. Yvan Chouinard*
Affiliation:
Département des sciences animales, Université Laval, Québec, Québec G1V 0A6, Canada
*
*For correspondence; e-mail: yvan.chouinard@fsaa.ulaval.ca
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Abstract

To evaluate the interaction between the levels of dietary concentrate and linseed oil (LO) on milk fatty acid (FA) profile, 24 Holstein cows were used in a randomised complete block design based on days in milk, with a 2×2 factorial arrangement of treatments. Within each block, cows were fed one of four experimental diets containing 30% concentrate (LC) or 70% concentrate (HC), without LO (NLO) or with LO supplemented at 3% of dietary dry matter. Milk FA profiles were analysed with a special emphasis on the intermediates of the predominant trans-11, and a putative trans-13 pathways of ruminal biohydrogenation of cis-9, cis-12, cis-15 18:3. Feeding LO increased the concentrations of cis-9, cis-12, cis-15 18:3 and trans-11, cis-15 18:2 in milk fat, and these increases were of a higher magnitude when LO was added in HC as compared with LC diet (interaction of LO by concentrate). A treatment interaction was also observed for the level of trans-11 18:1 which was higher when feeding LO, but for which the increase was more pronounced with the LC as compared with HC diet. The concentrations of cis-15 18:1 and cis-9, trans-11, cis-15 18:3 were higher in cows fed LO, but feeding HC diets decreased milk fat content of cis-15 18:1 and a tendency for a decrease in cis-9, trans-11, cis-15 18:3 was apparent. Feeding LO increased milk fat content of trans-13 18:1 and cis-9, trans-13 18:2, while the concentrations of these two isomers were not affected by the level of dietary concentrates. The isomer cis-9, trans-13, cis-15 18:3 has not been detected in any of the milk samples. In conclusion, interactions were observed between LO and dietary concentrates on the proportions of some intermediates of the trans-11 biohydrogenation pathway. The presence of trans-13 18:1 and cis-9, trans-13 18:2 supports the existence of a trans-13 pathway, but an 18:3 intermediate with a trans-13 double bond has not been identified.

Keywords

Dairy cowflaxseedbiohydrogenationforage to concentrate ratio
Type
Research Article
Information
Journal of Dairy Research , Volume 81 , Issue 1 , February 2014 , pp. 82 - 90
Copyright
Copyright © Proprietors of Journal of Dairy Research 2014 

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References

Akraim, F, Nicot, MC, Juaneda, P & Enjalbert, F 2007 Conjugated linolenic acid (CLnA), conjugated linoleic acid (CLA) and other biohydrogenation intermediates in plasma and milk fat of cows fed raw or extruded linseed. Animal 1 835–843 Google Scholar
Allen, MS 2000 Effects of diet on short-term regulation of feed intake by lactating dairy cattle. Journal of Dairy Science 83 1598–1624 CrossRefGoogle ScholarPubMed
AOAC International 2012 Official Methods of Analysis, 19th edition. Gaithersburg, MD: Association of Official Analytical Chemists International Google Scholar
Bauman, DE & Griinari, JM 2001 Regulation and nutritional manipulation of milk fat: low-fat milk syndrome. Livestock Production Science 70 15–29 CrossRefGoogle Scholar
Benchaar, C, Romero-Pérez, GA, Chouinard, PY, Hassanat, F, Eugene, M, Petit, HV & Côrtes, C 2012 Supplementation of increasing amounts of linseed oil to dairy cows fed total mixed rations: effects on digestion, ruminal fermentation characteristics, protozoal populations, and milk fatty acid composition. Journal of Dairy Science 95 4578–4590 CrossRefGoogle ScholarPubMed
Bichi, E, Toral, PG, Hervás, G, Gómez-Córtes, P, Juárez, M & de la Fuente, MA 2012 Inhibition of Δ9-desaturase activity with sterculic acid: effect on the endogenous synthesis of cis-9 18:1 and cis-9, trans-11 18:2 in dairy sheep. Journal of Dairy Science 95 5242–5252 CrossRefGoogle Scholar
Canadian Council on Animal Care 1993 Guide to Care and Use of Experimental Animals, Vol. 1 (Eds Offert, ED, Cross, BM & McWilliam, AA). Ottawa, ON, Canada Google Scholar
Chilliard, Y, Martin, C, Rouel, J & Doreau, M 2009 Milk fatty acid in dairy cows fed whole crude linseed, extruded linseed, or linseed oil, and their relationship with methane output. Journal of Dairy Science 92 5199–5211 CrossRefGoogle ScholarPubMed
Chouinard, PY, Lévesque, J, Girard, V & Brisson, GJ 1997 Dietary soybeans extruded at different temperatures: milk composition and in situ fatty acid reactions. Journal of Dairy Science 80 2913–2924 Google Scholar
Destaillats, F, Trottier, JP, Galvez, JMG & Angers, P 2005 Analysis of α-linolenic acid biohydrogenation intermediates in milk fat with emphasis on conjugated linolenic acids. Journal of Dairy Science 88 3231–3239 CrossRefGoogle ScholarPubMed
Emery, RS 1978 Feeding for increased milk protein. Journal of Dairy Science 61 825–828 CrossRefGoogle Scholar
Flachowsky, G, Erdmann, K, Hüther, L, Jahreis, G, Möckel, P & Lebzien, P 2006 Influence of roughage/concentrate ratio and linseed oil on the concentration of trans-fatty acids and conjugated linoleic acid in duodenal chyme and milk fat of late lactating cows. Archives of Animal Nutrition 60 501–511 Google Scholar
Gómez-Cortés, P, Tyburczy, C, Brenna, JT, Juárez, M & de la Fuente, MA 2009 Characterization of cis-9 trans-11 trans-15 C18:3 in milk fat by GC and covalent adduct chemical ionization tandem MS. Journal of Lipid Research 50 2412–2420 CrossRefGoogle ScholarPubMed
Griinari, JM & Bauman, DE 1999 Biosynthesis of conjugated linoleic acid and its incorporation into meat and milk in ruminants. In Advances in Conjugated Linoleic Acid Research, Vol 1, pp. 180–200 (Eds Yurawecz, MP, Mossoba, MM, Kramer, JKG, Pariza, MW, Nelson, GJ). Champaign, IL, USA: AOCSPress Google Scholar
Griinari, JM, Dwyer, DA, McGuire, MA, Bauman, DE, Palmquist, DL & Nurmela, KVV 1998 Trans-octadecanoic acids and milk fat depression in lactating dairy cows. Journal of Dairy Science 81 1251–1261 Google Scholar
Griinari, JM, Corl, BA, Lacy, SH, Chouinard, PY, Nurmela, KVV & Bauman, DE 2000 Conjugated linoleic acid is synthesized endogenously in lactating dairy cows by Δ9 desaturase. Journal of Nutrition 130 2285–2291 CrossRefGoogle Scholar
Harfoot, CG 1981 Lipid metabolism in the rumen. In Lipid Metabolism in Ruminant Animals, pp. 21–55 (Ed. Christie, WW). New York, NY, USA: Pergamon Press.CrossRefGoogle Scholar
Hurtaud, C, Faucon, F, Couvreur, S & Peyraud, JL 2010 Linear relationship between increasing amounts of extruded linseed in dairy cow diet and milk fatty acid composition and butter properties. Journal of Dairy Science 93 1429–1443 Google Scholar
Kramer, JKG, Hernandez, M, Cruz-Hernandez, C, Kraft, J & Dugan, MER 2008 Combining results of two GC separations partly achieves determination of all cis and trans 16:1, 18:1, 18:2 and 18:3 except CLA isomers of milk fat as demonstrated using Ag-ion SPE fractionation. Lipids 43 259–273 CrossRefGoogle Scholar
Lee, YJ & Jenkins, TC 2011 Biohydrogenation of linolenic acid to stearic acid by the rumen microbial population yields multiple intermediate conjugated diene isomers. Journal of Nutrition 141 1445–1450 CrossRefGoogle ScholarPubMed
Lerch, S, Shingfield, KJ, Ferlay, A, Vanhatalo, A & Chilliard, Y 2012 Rapeseed or linseed in grass-based diets: effects on conjugated linoleic and conjugated linolenic acid isomers in milk fat from Holstein cows over 2 consecutive lactations. Journal of Dairy Science 95 7269–7287 CrossRefGoogle ScholarPubMed
Loor, JJ, Ueda, K, Ferlay, A, Chilliard, Y & Doreau, M 2004 Biohydrogenation, duodenal flow, and intestinal digestibility of trans fatty acids and conjugated linoleic acids in response to dietary forage:concentrate ratio and linseed oil in dairy cows. Journal of Dairy Science 87 2472–2485 Google Scholar
Loor, JJ, Ferlay, A, Ollier, A, Doreau, M & Chilliard, Y 2005 Relationship among trans and conjugated fatty acids and bovine milk fat yield due to dietary concentrate and linseed oil. Journal of Dairy Science 88 726–740 Google Scholar
Martin, C, Rouel, J, Jouany, JP, Doreau, M & Chilliard, Y 2008 Methane output and diet digestibility in response to feeding dairy cows crude linseed, extruded linseed, or linseed oil. Journal of Animal Science 86 2642–2650 Google Scholar
Petit, HV 2010 Review: feed intake, milk production and milk composition of dairy cows fed flaxseed. Canadian Journal Animal Science 90 115–127 Google Scholar
Petit, HV, Dewhurst, RJ, Scollan, ND, Proulx, JG, Khalid, M, Haresign, W, Twagiramungu, H & Mann, GE 2002 Milk production and composition, ovarian function, and prostaglandin secretion of dairy cows fed omega-3 fats. Journal of Dairy Science 85 889–899 CrossRefGoogle ScholarPubMed
Plourde, M, Destaillats, F, Chouinard, PY & Angers, P 2007 Conjugated α-linolenic acid isomers in bovine milk and muscle. Journal of Dairy Science 90 5269–5275 Google Scholar
Precht, D, Molkentin, J, McGuire, MA, McGuire, MK & Jensen, RG 2001 Overestimates of oleic and linoleic acid contents in materials containing trans fatty acids and analyzed with short packed gas chromatographic columns. Lipids 36 213–216 Google Scholar
Rego, OA, Alves, SP, Antunes, LMS, Rosa, HJD, Alfaia, CFM, Prates, JAM, Cabrita, ARJ, Fonseca, AJM & Bessa, RJB 2009 Rumen biohydrogenation-derived fatty acids in milk fat from grazing dairy cows supplemented with rapeseed, sunflower, or linseed oils. Journal of Dairy Science 92 4530–4540 Google Scholar
Shingfield, KJ, Bernard, L, Leroux, C & Chilliard, Y 2010 Role of trans fatty acids in the nutritional regulation of mammary lipogenesis in ruminants. Animal 4 1140–1166 CrossRefGoogle ScholarPubMed
Sterk, A, Johansson, BEO, Taweel, HZH, Murphy, M, van Vuuren, AM, Hendriks, WH & Dijkstra, J 2011 Effects of forage type, forage to concentrate ratio, and crushed linseed supplementation on milk fatty acid profile in lactating dairy cows. Journal of Dairy Science 94 6078–6091 CrossRefGoogle ScholarPubMed
Sukhija, PS & Palmquist, DL 1988 Rapid method for determination of total fatty acid content and composition of feedstuffs and feces. Journal of Agricultural and Food Chemistry 36 1202–1206 CrossRefGoogle Scholar
Undersander, D, Mertens, DR & Thiex, N 1993 Forage Analyses Procedures. Omaha, NE: National Forage Testing Association Google Scholar
Van Soest, PJ, Robertson, JB & Lewis, BA 1991 Methods for dietary fiber, neutral detergent fiber and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74 3583–3597 CrossRefGoogle ScholarPubMed
Zened, A, Troegeler-Meynadier, A, Nicot, MC, Combes, S, Cauquil, L, Farizon, Y & Enjalbert, F 2011 Starch and oil in the donor cow diet and starch in substrate differently affect the in vitro ruminal biohydrogenation of linoleic and linolenic acids. Journal of Dairy Science 94 5634–5645 CrossRefGoogle ScholarPubMed