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Developments in column chromatography for the separation and characterization of casein micelles

Published online by Cambridge University Press:  01 June 2009

Thomas C. A. McGann ,
Robert D. Kearney  and
William J. Donnelly
Thomas C. A. McGann
Affiliation:
Agricultural Institute, Moorepark, Fermoy, County Cork, Irish Republic
Robert D. Kearney
Affiliation:
Agricultural Institute, Moorepark, Fermoy, County Cork, Irish Republic
William J. Donnelly
Affiliation:
Agricultural Institute, Moorepark, Fermoy, County Cork, Irish Republic
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Summary

Skim-milk was fractionated by permeation chromatography on CPG-10 (300 nm) at 20 °C with synthetic milk serum. The elution profile, which was highly reproducible, showed 3 partly resolved peaks. On treatment with 0.1% glutaralde-hyde before chromatography, a similar profile was obtained indicating no appreciable micellar dissociation during chromatography of the milk. The casein composition of the eluent fractions, determined by quantitative chromatography on hydroxyapatite, showed that 12.1% of total casein was associated with the low molecular weight peak. The micelle content of ²- and κ-casein increased with increasing elution volume, and the ±s/κ ratio showed a progressive diminution from 4·3 to 2·3. Large differences were found in the colloidal phosphate contents and the weight average diameters of the micelle fractions which clearly show that CPG-10 fractionates on the basis of molecular size.

Type
Section D. Casein Micelles
Information
Journal of Dairy Research , Volume 46 , Issue 2: Symposium on the Physics and Chemistry of Milk Proteins , April 1979 , pp. 307 - 311
Copyright
Copyright © Proprietors of Journal of Dairy Research 1979

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References

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