Hostname: page-component-78c5997874-xbtfd Total loading time: 0 Render date: 2024-11-10T17:15:49.716Z Has data issue: false hasContentIssue false

Effect of fermented milk containing Lactobacillus acidophilus and Bifidobacterium longum on plasma lipids of women with normal or moderately elevated cholesterol

Published online by Cambridge University Press:  13 October 2009

Sara Andrade
Affiliation:
Regional Health Service – Health Centre Dr. Rui Adriano Ferreira Freitas, Av. Do Colégio Militar, n.°27, 9000-135 Funchal, Portugal
Nuno Borges*
Affiliation:
Faculty of Nutrition and Food Sciences, University of Porto, Rua Dr. Roberto Frias, 4200-465, Porto, Portugal
*
*For correspondence; e-mail: nunoborges@fcna.up.pt

Abstract

This study aimed to evaluate the effect of milk fermented with Lactobacillus acidophilus 145 and Bifidobacterium longum BB536 on plasma lipids in a sample of adult women. A double-blind, placebo controlled, cross-over study (two periods of four weeks each separated by a 1-week washout period) was performed in 34 women, aged between 18 and 65 years. Group A consumed 125 g fermented milk three times a day for the first 4 weeks while group B consumed regular yoghurt under the same conditions. (Groups A and B switched products for the second treatment period). Women taking the test product with a baseline total cholesterol above 190 mg/dl showed a significant reduction in LDL cholesterol. HDL cholesterol was also reduced by the test product. We conclude that the fermented milk may help to reduce LDL levels in hypercholesterolemic adult women.

Type
Research Article
Copyright
Copyright © Proprietors of Journal of Dairy Research 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Agerholm-Larsen, L, Raben, A, Haulrik, N, Hansen, AS, Manders, M & Astrup, A 2000 Effect of 8 week intake of probiotic milk products on risk factors for cardiovascular diseases. European Journal of Clinical Nutrition 54 288297CrossRefGoogle ScholarPubMed
Akalin, AS, Gonc, S & Duzel, S 1997 Influence of yogurt and acidophilus yogurt on serum cholesterol levels in mice. Journal of Dairy Science 80 27212725CrossRefGoogle ScholarPubMed
Allain, CC, Poon, LS, Chan, CS, Richmond, W & Fu, PC 1974 Enzymatic determination of total serum cholesterol. Clinical Chemistry 20 470475Google Scholar
Anderson, JW & Gilliland, SE 1999 Effect of fermented milk (yogurt) containing Lactobacillus acidophilus L1 on serum cholesterol in hypercholesterolemic humans. Journal of the American College of Nutrition 18 4350CrossRefGoogle ScholarPubMed
Ashar, MN & Prajapati, JB 2000 Verification of hypocholesterolemic effect of fermented milk on human subjects with different cholesterol levels. Folia Microbiologica (Praha) 45 263268CrossRefGoogle ScholarPubMed
Bertolami, MC, Faludi, AA & Batlouni, M 1999 Evaluation of the effects of a new fermented milk product (Gaio) on primary hypercholesterolemia. European Journal of Clinical Nutrition 53 97–101Google Scholar
Brashears, MM, Gilliland, SE & Buck, LM 1998 Bile salt deconjugation and cholesterol removal from media by Lactobacillus casei. Journal of Dairy Science 81 21032110Google Scholar
Buck, LM & Gilliland, SE 1994 Comparisons of freshly isolated strains of Lactobacillus acidophilus of human intestinal origin for ability to assimilate cholesterol during growth. Journal of Dairy Scence 77 29252933Google Scholar
Cobb, MM, Teitlebaum, H, Risch, N, Jekel, J & Ostfeld, A 1992 Influence of dietary fat, apolipoprotein E phenotype, and sex on plasma lipoprotein levels. Circulation 86 849857CrossRefGoogle ScholarPubMed
Dambekodi, PC & Gilliland, SE 1998 Incorporation of cholesterol into the cellular membrane of Bifidobacterium longum. Journal of Dairy Science 81 18181824Google Scholar
Danielson, AD, Peo, ER Jr, Shahani, KM, Lewis, AJ, Whalen, PJ & Amer, MA 1989 Anticholesteremic property of Lactobacillus acidophilus yogurt fed to mature boars. Journal of Animal Science 67 966974CrossRefGoogle ScholarPubMed
De Backer, G, Ambrosioni, E, Borch-Johnsen, K, Brotons, C, Cifkova, R, Dallongeville, J, Ebrahim, S, Faergeman, O, Graham, I, Mancia, G, Manger Cats, V, Orth-Gomer, K, Perk, J, Pyorala, K, Rodicio, JL, Sans, S, Sansoy, V, Sechtem, U, Silber, S, Thomsen, T & Wood, D 2003 European guidelines on cardiovascular disease prevention in clinical practice. Third Joint Task Force of European and Other Societies on Cardiovascular Disease Prevention in Clinical Practice. European Heart Journal 24 16011610CrossRefGoogle ScholarPubMed
De Rodas, BZ, Gilliland, SE & Maxwell, CV 1996 Hypocholesterolemic action of Lactobacillus acidophilus ATCC 43121 and calcium in swine with hypercholesterolemia induced by diet. Journal of Dairy Science 79 21212128CrossRefGoogle ScholarPubMed
de Roos, NM & Katan, MB 2000 Effects of probiotic bacteria on diarrhea, lipid metabolism, and carcinogenesis: a review of papers published between 1988 and 1998. American Journal of Clinical Nutrition 71 405411CrossRefGoogle ScholarPubMed
de Roos, NM, Schouten, G & Katan, MB 1999 Yoghurt enriched with Lactobacillus acidophilus does not lower blood lipids in healthy men and women with normal to borderline high serum cholesterol levels. European Journal of Clinical Nutrition 53 277280Google Scholar
De Smet, I, De Boever, P & Verstraete, W 1998 Cholesterol lowering in pigs through enhanced bacterial bile salt hydrolase activity. British Journal of Nutrition 79 185194CrossRefGoogle ScholarPubMed
Dreon, DM, Fernstrom, HA, Miller, B & Krauss, RM 1995 Apolipoprotein E isoform phenotype and LDL subclass response to a reduced-fat diet. Arteriosclererosis, Thrombosis and Vascular Biology 15 105111CrossRefGoogle ScholarPubMed
Dreon, DM & Krauss, RM 1997 Diet-gene interactions in human lipoprotein metabolism. Journal of the American College of Nutrition 16 313324CrossRefGoogle ScholarPubMed
FAO/WHO 2001 Report of a Joint FAO/WHO Expert Consultation on Evaluation of Health and Nutritional Properties of Probiotics in Food Including Powder Milk with Live Lactic Acid Bacteria. Edited by FAO/WHO. Cordoba, Argentina: FAO/WHO.Google Scholar
Harrison, VC & Peat, G 1975 Serum cholesterol and bowel flora in the newborn. American Journal of Clinical Nutrition 28 13511355Google Scholar
IDFGroup-E104, L.a.b.a.s. 1999 Guideline for the Enumeration of Bifidobacteria in fermented Dairy Products. Bulletin of the International Dairy Federation 340 1923Google Scholar
ISO-20128 2006 Milk products – Enumeration of presumptive Lactobacilus acdophilus on a aselective medium – Colony-count technique at 37°C. International Standards Organization.Google Scholar
Isolauri, E 2001 Probiotics in human disease. American Journal of Clinical Nutrition 73 1142S1146SGoogle Scholar
Kiessling, G, Schneider, J & Jahreis, G 2002 Long-term consumption of fermented dairy products over 6 months increases HDL cholesterol. European Journal of Clinical Nutrition 56 843849CrossRefGoogle ScholarPubMed
Kimoto, H, Ohmomo, S & Okamoto, T 2002 Cholesterol removal from media by lactococci. Journal of Dairy Science 85 31823188CrossRefGoogle ScholarPubMed
Klaver, FA & van der Meer, R 1993 The assumed assimilation of cholesterol by Lactobacilli and Bifidobacterium bifidum is due to their bile salt-deconjugating activity. Applied and Environmental Microbiology 59 11201124CrossRefGoogle ScholarPubMed
Lewis, SJ & Burmeister, S 2005 A double-blind placebo-controlled study of the effects of Lactobacillus acidophilus on plasma lipids. European Journal of Clinical Nutrition 59 776780Google Scholar
Liong, MT & Shah, NP 2005 Acid and bile tolerance and cholesterol removal ability of lactobacilli strains. Journal of Dairy Science 88 5566Google Scholar
Lopez-Miranda, J, Ordovas, JM, Mata, P, Lichtenstein, AH, Clevidence, B, Judd, JT & Schaefer, EJ 1994 Effect of apolipoprotein E phenotype on diet-induced lowering of plasma low density lipoprotein cholesterol. Journal of Lipid Research 35 19651975Google Scholar
Mann, GV & Spoerry, A 1974 Studies of a surfactant and cholesteremia in the Maasai. American Journal of Clinical Nutrition 27 464469Google Scholar
Masson, LF, McNeill, G & Avenell, A 2003 Genetic variation and the lipid response to dietary intervention: a systematic review. American Journal of Clinical Nutrition 77 10981111Google Scholar
Pereira, DI & Gibson, GR 2002a Cholesterol assimilation by lactic acid bacteria and bifidobacteria isolated from the human gut. Applied and Environmental Microbiology 68 46894693CrossRefGoogle ScholarPubMed
Pereira, DI & Gibson, GR 2002b Effects of consumption of probiotics and prebiotics on serum lipid levels in humans. Critical Reviews in Biochemistry and Molecular Biology 37 259281CrossRefGoogle ScholarPubMed
Richelsen, B, Kristensen, K & Pedersen, SB 1996 Long-term (6 months) effect of a new fermented milk product on the level of plasma lipoproteins – a placebo-controlled and double blind study. European Journal of Clinical Nutrition 50 811815Google ScholarPubMed
Saarela, M, Mogensen, G, Fonden, R, Matto, J & Mattila-Sandholm, T 2000 Probiotic bacteria: safety, functional and technological properties. Journal of Biotechnology 84 197215Google Scholar
Schaafsma, G, Meuling, WJ, van Dokkum, W & Bouley, C 1998 Effects of a milk product, fermented by Lactobacillus acidophilus and with fructo-oligosaccharides added, on blood lipids in male volunteers. European Journal of Clinical Nutrition 52 436440Google Scholar
Schrezenmeir, J & de Vrese, M 2001 Probiotics, prebiotics, and synbiotics – approaching a definition. American Journal of Clinical Nutrition 73(2 Suppl) 361S364SGoogle Scholar
Shaper, AG, Jones, KW, Jones, M & Kyobe, J 1963 Serum Lipids in Three Nomadic Tribes of Northern Kenya. American Journal of Clinical Nutrition 13 135146CrossRefGoogle ScholarPubMed
SPSS (2004) Statistical Package for Social Sciences. SPSS Inc.Google Scholar
St-Onge, MP, Farnworth, ER & Jones, PJ 2000 Consumption of fermented and nonfermented dairy products: effects on cholesterol concentrations and metabolism. American Journal of Clinical Nutrition 71 674681Google Scholar
St-Onge, MP, Farnworth, ER, Savard, T, Chabot, D, Mafu, A & Jones, PJ 2002 Kefir consumption does not alter plasma lipid levels or cholesterol fractional synthesis rates relative to milk in hyperlipidemic men: a randomized controlled trial. BMC Complementary and Alternative Medicine 2 1Google Scholar
Tabas, I 2002 Cholesterol in health and disease. Journal of Clinical Investigation 110 583590Google Scholar
van Vlijmen, BJ, van den Maagdenberg, AM, Gijbels, MJ, van der Boom, H, HogenEsch, H, Frants, RR, Hofker, MH & Havekes, LM 1994 Diet-induced hyperlipoproteinemia and atherosclerosis in apolipoprotein E3-Leiden transgenic mice. Journal of Clinical Investigation 93 14031410CrossRefGoogle ScholarPubMed
Walker, DK & Gilliland, SE 1993 Relationship among bile tolerance, bile salt deconjugation, and assimilation of cholesterol by Lactobacillus acidophilus. Journal of Dairy Science 76 956961Google Scholar
Weggemans, RM, Zock, PL, Ordovas, JM, Pedro-Botet, J & Katan, MB 2001 Apoprotein E genotype and the response of serum cholesterol to dietary fat, cholesterol and cafestol. Atherosclerosis 154 547555CrossRefGoogle ScholarPubMed
Xiao, JZ, Kondo, S, Takahashi, N, Miyaji, K, Oshida, K, Hiramatsu, A, Iwatsuki, K, Kokubo, S & Hosono, A 2003 Effects of milk products fermented by Bifidobacterium longum on blood lipids in rats and healthy adult male volunteers. Journal of Dairy Science 86 24522461Google Scholar