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A gel electrophoretic study of caprine casein

Published online by Cambridge University Press:  01 June 2009

Francesco Addeo ,
Rosalba Mauriello  and
Aldo Di Luccia
Francesco Addeo
Affiliation:
Istituto Industrie Agrarie dell'Università di Napoli, 80055 Portici, Italy
Rosalba Mauriello
Affiliation:
Istituto Industrie Agrarie dell'Università di Napoli, 80055 Portici, Italy
Aldo Di Luccia
Affiliation:
Istituto Ricerche Adattamento Bovini e Bufali Ambiente del Mezzogiorno, CNR, 80147 Naples, Italy
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Summary

To compare the resolving power of starch-urea gel (SUGE) and polyacrylamide-agarose gel electrophoresis (PAAGE) in caprine casein analysis, polyacrylamide gel isoelectric focusing (PAGIF) was used as reference method. The PAAGE or SUGE patterns in the first dimension were allowed to migrate by PAGIF in an orthogonal direction giving rise to two-dimensional (2-D) separations. Using this procedure, some individual bands considered to be homogeneous by SUGE or PAAGE were found to be complex mixtures of casein components. A detailed analysis of the analytical capabilities of SUGE and PAAGE was performed. Procedures based on the densitometric reading of SUGE and PAAGE patterns seemed to give inaccurate results on the quantitative composition of caprine casein. Among the electrophoretic methods assayed, the 2-D procedure gave the best resolution of casein fractions.

Type
Original articles
Information
Journal of Dairy Research , Volume 55 , Issue 3 , August 1988 , pp. 413 - 421
Copyright
Copyright © Proprietors of Journal of Dairy Research 1988

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References

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