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Genetic variation at the goat hormone-sensitive lipase (LIPE) gene and its association with milk yield and composition

Published online by Cambridge University Press:  12 April 2010

Ali Zidi ,
Víctor M Fernández-Cabanás ,
Juan Carrizosa ,
Jordi Jordana ,
Baltasar Urrutia ,
Oliva Polvillo ,
Pedro González-Redondo ,
David Gallardo ,
Marcel Amills  and
Juan M Serradilla
Ali Zidi
Affiliation:
Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 08193Bellaterra, Spain
Víctor M Fernández-Cabanás
Affiliation:
Departamento de Ciencias Agroforestales, Escuela Universitaria de Ingeniería Técnica Agrícola-Universidad de Sevilla, Carretera Utrera, km. 1. 41013 Sevilla, Spain
Juan Carrizosa
Affiliation:
Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA). Estación Sericícola. La Alberca, Murcia, Spain
Jordi Jordana
Affiliation:
Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 08193Bellaterra, Spain
Baltasar Urrutia
Affiliation:
Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA). Estación Sericícola. La Alberca, Murcia, Spain
Oliva Polvillo
Affiliation:
Departamento de Ciencias Agroforestales, Escuela Universitaria de Ingeniería Técnica Agrícola-Universidad de Sevilla, Carretera Utrera, km. 1. 41013 Sevilla, Spain
Pedro González-Redondo
Affiliation:
Departamento de Ciencias Agroforestales, Escuela Universitaria de Ingeniería Técnica Agrícola-Universidad de Sevilla, Carretera Utrera, km. 1. 41013 Sevilla, Spain
David Gallardo
Affiliation:
Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 08193Bellaterra, Spain
Marcel Amills*
Affiliation:
Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 08193Bellaterra, Spain
Juan M Serradilla*
Affiliation:
Departamento de Producción Animal, Campus de Rabanales, Universidad de Córdoba, 14071Córdoba, Spain
*
*For correspondence; e-mail: Marcel.Amills@uab.cat
*For correspondence; e-mail: pa1semaj@uco.es
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Abstract

Hormone-sensitive lipase (LIPE) plays a fundamental role in the regulation of energy balance by releasing free fatty acids from adipose triacylglycerol stores. These fatty acids can be subsequently transferred to other body compartments to be oxidized or employed in other biochemical reactions. This enzymic function is particularly important in lactating animals because the synthesis of milk components involves the mobilization of lipid depots to satisfy the large energy demands of the mammary gland. In the current study, we partially sequenced the goat LIPE gene in several individuals. In doing so, we identified two synonymous polymorphisms at exons 2 (c.327C>A>T, triallelic polymorphism) and 3 (c.558C>T). Moreover, we found a mis-sense polymorphism at exon 6 (c.1162G>T) that involves an alanine to serine substitution at position 388. Analysis with Polyphen and Panther softwares revealed that this amino acid replacement is expected to be neutral. Performance of an association analysis with a variety of milk traits revealed that goat LIPE genotype has highly suggestive effects on milk yield (P=0·0032) as well as on C18:3 n-6g (P=0·0051), trans-10 cis-12 CLA (P=0·007) and C12:0 (P=0·0084) milk contents. These associations are concordant with the preference of LIPE to selectively mobilize medium-chain and unsaturated fatty acids.

Keywords

Hormone-sensitive lipasegenemilk traitsgoat
Type
Research Article
Information
Journal of Dairy Research , Volume 77 , Issue 2 , May 2010 , pp. 190 - 198
Copyright
Copyright © Proprietors of Journal of Dairy Research 2010

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