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Metataxonomics contributes to unravel the microbiota of a Brazilian dairy

Published online by Cambridge University Press:  04 September 2020

Diego Araújo Frazilio ,
Otávio Guilherme Gonçalves de Almeida ,
Carlos Augusto Fernandes de Oliveira ,
Sarah Hwa In Lee ,
Carlos Humberto Corassin ,
Virgínia Farias Alves  and
Elaine Cristina Pereira De Martinis
Diego Araújo Frazilio
Affiliation:
Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil
Otávio Guilherme Gonçalves de Almeida
Affiliation:
Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil
Carlos Augusto Fernandes de Oliveira
Affiliation:
Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, São Paulo, Brazil
Sarah Hwa In Lee
Affiliation:
Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, São Paulo, Brazil
Carlos Humberto Corassin
Affiliation:
Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, São Paulo, Brazil
Virgínia Farias Alves
Affiliation:
Faculdade de Farmácia, Universidade Federal de Goiás, Goiânia, Goiás, Brazil
Elaine Cristina Pereira De Martinis*
Affiliation:
Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil
*
Author for correspondence: Elaine Cristina Pereira De Martinis, Email: edemarti@usp.br
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Abstract

For this research communication, 90 samples of a Brazilian dairy were combined into four groups (raw material, final product, food-contact and non-food contact surfaces) and analyzed by metataxonomics based on 16S rRNA gene sequencing. The results showed high alpha-diversity indexes for final product and non-food contact surfaces but, overall, beta-diversity indexes were low. The samples were separated in two main clusters, and the core microbiota was composed by Macrococcus, Alkaliphilus, Vagococcus, Lactobacillus, Marinilactibacillus, Streptococcus, Lysinibacillus, Staphylococcus, Clostridium, Halomonas, Lactococcus, Enterococcus, Bacillus and Psychrobacter. These results highlight that rare taxa occur in dairies, and this may aid the development of strategies for food protection.

Keywords

dairy microbiotalactic acid bacteriafood metataxonomicscheese microbiomemilk microbiome
Type
Research Article
Information
Journal of Dairy Research , Volume 87 , Issue 3 , August 2020 , pp. 360 - 363
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation.

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