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Occurrence of methicillin-resistant Staphylococcus aureus (MRSA) in ‘coalho’ cheese produced in Brazil

Published online by Cambridge University Press:  21 February 2022

Felipe R. M. de Aguiar ,
Larissa Q. Rocha ,
Morgana M. O. Barboza ,
Tiago L. Sampaio ,
Nádia A. P. Nogueira  and
Ramon R. P. P. B. de Menezes
Felipe R. M. de Aguiar
Affiliation:
Department of Pharmaceutical Sciences, Federal University of Ceará, Fortaleza, Brazil
Larissa Q. Rocha
Affiliation:
Department of Pharmaceutical Sciences, Federal University of Ceará, Fortaleza, Brazil
Morgana M. O. Barboza
Affiliation:
Department of Biotechnology, Federal University of Ceará, Fortaleza, Brazil
Tiago L. Sampaio
Affiliation:
Department of Clinical and Toxicological Analysis, Federal University of Ceará, Fortaleza, Brazil
Nádia A. P. Nogueira
Affiliation:
Department of Clinical and Toxicological Analysis, Federal University of Ceará, Fortaleza, Brazil
Ramon R. P. P. B. de Menezes*
Affiliation:
Department of Clinical and Toxicological Analysis, Federal University of Ceará, Fortaleza, Brazil
*
Author for correspondence: Ramon RPPB de Menezes, Email: ramonppessoa@ufc.br
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Abstract

The experiments reported in this research communication analysed the presence of methicillin-resistant Staphylococcus aureus (MRSA) in 112 samples of ‘coalho’ cheese, from 56 dairy producing farms in 28 cities in all mesoregions of the State of Ceará, Brazil. To assess antimicrobial resistance we also examined the presence of genes encoding enterotoxins and toxic shock syndrome toxin, as well as the presence of the blaZ gene for β-lactamases, and resistance to oxacillin. The research found 69 isolates of S. aureus, of which 13.04% had the mecA gene encoding the penicillin-binding protein, which confers resistance to methicillin, in cheese samples from 6 different cities. This included the state capital, Fortaleza, which had the largest prevalence (23.19%) of mecA positive isolates. It was also found that 55.07% of the isolates of S. aureus had the blaZ gene, and 7.25% demonstrated resistance to oxacillin in the plate disc diffusion tests. We did not show the presence of isolates carrying toxigenic genes. The findings suggest that strict supervision of production processes in the dairy industry is necessary in all production scale processes, thus preventing contamination and possible problems for consumers.

Keywords

Dairy productsEnterotoxinsMRSAStaphylococcus aureus
Type
Research Article
Information
Journal of Dairy Research , Volume 89 , Issue 1 , February 2022 , pp. 100 - 103
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation.

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