Hostname: page-component-cd9895bd7-lnqnp Total loading time: 0 Render date: 2024-12-26T20:37:08.731Z Has data issue: false hasContentIssue false

Use of California Mastitis Test, N-acetyl-β-glucosaminidase, and antitrypsin to diagnose caprine subclinical mastitis

Published online by Cambridge University Press:  01 June 2009

Päivi Maisi
Affiliation:
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Hämeentie 57, 00551 Helsinki, Finland
Inna Riipinen
Affiliation:
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Hämeentie 57, 00551 Helsinki, Finland

Summary

Analysis of 448 milk samples (11 herds) from caprine udder halves showed that microorganisms were isolated from 21·8% of the samples. California Mastitis Test (GMT) and N-acetyl-β-glucosaminidase (NAGase) were superior to antitrypsin in detecting subclinical infections. Coagulase-negative staphylococci and micrococci were the main species isolated from halves showing no clinical disease. Coagulase-positive staphylococcal infections were associated with a significant increase of all inflammatory parameters. Significantly increased CMT and NAGase occurred when streptococci, other staphylococci or micrococci were present. Infection within one half was reflected as an increase in the inflammatory parameters in the milk of the infected half as well as a slight increase in the inflammation parameters in the adjoining half.

Type
Original articles
Copyright
Copyright © Proprietors of Journal of Dairy Research 1988

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Honkanen-Buzalski, T., Katila, T. & Sandholm, M. 1981 Milk antitrypsin activity during clinical and experimental bovine mastitis. Acta Veterinaria Scandinavica 22 360368CrossRefGoogle ScholarPubMed
Hunter, A. C. 1984 Microflora and somatic cell content of goat milk. Veterinary Record 114 318320CrossRefGoogle ScholarPubMed
Kitchen, B. J., Middleton, G. & Salmon, M. 1978 Bovine milk N-acetyl-β-D-glucosaminidase and its significance in the detection of abnormal udder secretions. Journal of Dairy Research 45 1520CrossRefGoogle ScholarPubMed
Klastrup, O. 1975 Scandinavian recommendations on examination of quarter milk samples. International Dairy Federation Bulletin Document 85 4952Google Scholar
Klastrup, O. & Schmidt Madsen, P. 1974 [Scandinavian recommendations concerning examination of quarter samples for mastitis.] Nordisk Veterinærmedicin 26 197204Google Scholar
Lerondelle, C. & Poutrel, B. 1984 Characteristics of non-clinical mammary infections of goats. Annales de Recherches Vétérinaires 15 105112Google ScholarPubMed
Maisi, P., Junttila, J. & Seppänen, J. 1987 Detection of subclinical mastitis in ewes. British Veterinary Journal 143 402409CrossRefGoogle ScholarPubMed
Manser, P. A. 1986 Prevalence, causes and laboratory diagnosis of subclinical mastitis in the goat. Veterinary Record 118 552554CrossRefGoogle ScholarPubMed
Mattila, T. 1985 Diagnostic problems in bovine mastitis with special reference to new applications of milk antitrypsin, NAGase and bacterial growth. Thesis, College of Veterinary Medicine, HelsinkiGoogle Scholar
Nesbakken, T. 1976 [The cell count in milk of goats.] Nordisk Veterinaermedicin 28 550556Google ScholarPubMed
Park, Y. W. & Humphrey, R. D. 1986 Bacterial cell counts in goat milk and their correlations with somatic cell counts, percent fat, and protein. Journal of Dairy Science 69 3237CrossRefGoogle ScholarPubMed
Pettersen, K.-E. 1981 Cell content in goat's milk. Acta Veterinaria Scandinavica 22 226237CrossRefGoogle ScholarPubMed
Poutrel, B. & Lerondelle, C. 1983 Cell content of goat milk: California Mastitis Test, Coulter Counter, and Fossomatic for predicting half infection. Journal of Dairy Science 66 25752579CrossRefGoogle ScholarPubMed
Sandholm, M. 1983 Milk antitrypsin assay: a novel method of screening for mastitis. 3rd International Symposium of World Association of Veterinarian Laboratory Diagnosticians 2 571576Google Scholar
Schalm, O. W. & Noorlander, D. O. 1957 Experiments and observations leading to development of the California mastitis test. Journal of the American Veterinary Medical Association 130 199204Google ScholarPubMed
Smith, M. C. & Roguinsky, M. 1977 Mastitis and other diseases of the goat's udder. Journal of the American Veterinary Medical Association 171 12411248Google ScholarPubMed