Hostname: page-component-78c5997874-j824f Total loading time: 0 Render date: 2024-11-13T13:53:01.376Z Has data issue: false hasContentIssue false
  • English
  • Français

Leukocyte accumulation in sparganosis: further characterization of an eosinophil chemotactic factor of the plerocercoid of Spirometra erinacei

Published online by Cambridge University Press:  05 June 2009

Y. Horii ,
M. Owhashi ,
A. Ishii  and
K. Fujita
Y. Horii
Affiliation:
Department of Medical Zoology, Nagasaki University School of Medicine, Sakamoto-machi, Nagasaki 852, Japan
M. Owhashi
Affiliation:
Department of Parasitology, Miyazaki Medical College, Kiyotake, Miyazaki 889–16, Japan
A. Ishii
Affiliation:
Department of Parasitology, Okayama University Medical School, Shikata-cho, Okayama 700, Japan
K. Fujita
Affiliation:
Department of Medical Zoology, Nagasaki University School of Medicine, Sakamoto-machi, Nagasaki 852, Japan
Get access Rights & Permissions [Opens in a new window]

Abstract

An eosinophil chemotactic factor (ECF) was partially purified from plerocercoids of Spirometra erinacei by a combination of anion-exchange chromatography on DE52 and gel filtration on Sephacryl S–200. The molecular weight of ECF was estimated to be 25 000–45 000 by high-pressure liquid chromatography. The ECF was bound with concanavalin A-Sepharose. The ECF was sensitive to periodate oxidation and to heating (56°C, 30 min). On isoelectric focusing, eosinophil chemotactic activity was clearly revealed at pI 4·1. These results suggest that ECF of S. erinacei plerocercoid is an acidic glycoprotein. An intradermal injection of ECF induced eosinophil attractions in the normal guinea pig skin.

Keywords

Spirometra erinaceieosinophil chemotactic factor
Type
Research Article
Information
Journal of Helminthology , Volume 63 , Issue 1 , March 1989 , pp. 6 - 12
Copyright
Copyright © Cambridge University Press 1989

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Chi, J. G., Chi, S. H. & Lee, S. H. (1980) Histopathologic study on human sparganosis. Korean Journal of Parasitology, 18, 15–23.CrossRefGoogle Scholar
Horii, Y., Fujita, K. & Owhashi, M. (1986) Partial purification and characterization of eosinophil chemotactic factors from soluble extract of Fasciola species. American Journal of Veterinary Research, 47, 123–126.Google ScholarPubMed
Horii, Y., Ishii, A. & Owhashi, M. (1985a) In vitro and in vivo induction of neutrophil and eosinophil chemotactic responses by Schistosoma japonicum cercaria. American Journal of Tropical Medicine and Hygiene, 34, 513–518.CrossRefGoogle ScholarPubMed
Horii, Y., Ishii, A., Owhashi, M., Miyoshi, M. & Usui, M. (1985a) Neutrophilic nodules in the intestinal walls of Japanese monkeys associated with the neutrophil chemotactic activity of larval extracts and secretions of Oesophagostomum aculeatum. Research in Veterinary Science, 38, 115–119.CrossRefGoogle Scholar
Horii, Y., Owhashi, M., Ishii, A., Bandou, K. & Usui, M. (1984a) Leukocyte accumulation in sparganosis: Demonstration of eosinophil and neutrophil chemotactic factors from the plerocercoid of Spirometra erinacei in vivo and in vitro. American Journal of Tropical Medicine and Hygiene, 33, 138–143.CrossRefGoogle ScholarPubMed
Horii, Y., Owhashi, M., Ishii, A., Bandou, K. & Usui, M. (1984b) Eosinophil and neutrophil chemotactic activities of adult worm extracts of Schistosoma japonicum in vivo and in vitro. Journal of Parasitology, 70, 955–961.CrossRefGoogle ScholarPubMed
Kay, A. B. & Austen, K. F. (1971) The IgE-mediated release of an eosinophil leukocyte chemotactic factor from human lung. Journal of Immunology, 107, 899–902.CrossRefGoogle ScholarPubMed
Klesius, P. H., Haynes, T. B. & Cross, D. A. (1985) Chemotactic factors for eosinophils in soluble extracts of L3 stages of Ostertagia ostertagi. International Journal for Parasitology, 15, 517–522.CrossRefGoogle ScholarPubMed
Klesius, P. H., Haynes, T. B., Cross, D. A. & Ciordia, H. (1986) Ostertagia ostertagi: Excretory secretory chemotactic substance from infective larvae as the cause of eosinophil locomotion. Experimental Parasitology, 61, 120–125.CrossRefGoogle ScholarPubMed
Litt, M. (1963) Studies in experimental eosinophilia. V. Eosinophils in lymph nodes of guinea pigs following primary antigenic stimulation. American Journal of Pathology, 42, 529–549.Google ScholarPubMed
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951) Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 193, 265–275.CrossRefGoogle ScholarPubMed
Nawa, Y., Owhashi, M., Imai, J. & Abe, T. (1986) Chemotactic reactivity of eosinophils obtained from bone marrow and peritoneal cavity of cyclophosphamide-treated, Toxocara canis-infected mice. International Archives of Allergy and Applied Immunology, 80, 412–416.CrossRefGoogle ScholarPubMed
Owhashi, M., Horii, Y., Ishii, A. & Nawa, Y. (1986) Low molecular weight eosinophil chemotactic factor (ECF) in the serum of murine schistosomiasis japonica. International Archives of Allergy and Applied Immunology, 79, 178–181.CrossRefGoogle ScholarPubMed
Owhashi, M. & Ishii, A. (1982) Purification and characterization of a high molecular weight eosinophil chemotactic factor from Schistosoma japonicum eggs. Journal of Immunology, 129, 2226–2231.CrossRefGoogle ScholarPubMed
Tanaka, J., Baba, T. & Torisu, M. (1979) Ascaris and eosinophil. II. Isolation and characterization of eosinophil chemotactic factor and neutrophil chemotactic factor of parasite in Ascaris antigen. Journal of Immunology, 122, 302–308.CrossRefGoogle ScholarPubMed
Taylor, R. L. (1976) Sparganosis in the United States. Report of a case. American Journal of Clinical Pathology, 66, 560–564.CrossRefGoogle ScholarPubMed
Vesterberg, O. (1971) Isoelectric focusing of proteins. Methods of Enzymology, 22, 389–412.CrossRefGoogle Scholar