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The nature of antigens common to both the metacestodes of Taenia crassiceps and its laboratory host

Published online by Cambridge University Press:  18 November 2009

J. Chernin
J. Chernin
Affiliation:
Department of Biological Sciences, North East Surrey College of Technology, Reigate Road, Ewell, Surrey, England
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Abstract

Metacestodes of Taenia crassiceps were cultured in laboratory rats and mice, washed in glycine/hydrochloric acid (Gly/Hcl) buffer pH 2·4 and phosphate buffered saline (PBS) pH 7·2 and a soluble extract of the metacestodes (SEM) was prepared from larvae washed in both buffers. The SEM and the original washings were examined for host IgG and a common host antigen (CHA). CHA was present in both the SEM and Washings but IgG only in the washings and, in metacestodes from mice only in the Gly/HCl washings). Metacestodes washed in Gly/HCl were not viable.

Metacestodes were also maintained in vitro in a peptide nutrient agar and 0·9% saline at 37°C and a SEM was prepared from these; metacestodes from mice had neither host IgG nor CHA but those from rats had CHA but not IgG. SEM prepared from ‘normal’ metacestodes showed that the CHA consisted of at least two entities, only one having a common identity with the IgG. These results suggest that molecules of both host IgG and a common host antigen are present on the tegument of these parasites.

Type
Research Papers
Information
Journal of Helminthology , Volume 56 , Issue 4 , December 1982 , pp. 339 - 344
Copyright
Copyright © Cambridge University Press 1982

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