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A survey of entomopathogenic nematodes and their symbiotic bacteria in agricultural areas of northern Thailand

Published online by Cambridge University Press:  14 September 2020

J. Ardpairin ,
P. Muangpat ,
S. Sonpom ,
A. Dumidae ,
C. Subkrasae ,
S. Tandhavanant ,
A. Thanwisai  and
A. Vitta Open the ORCID record for A. Vitta [Opens in a new window]
J. Ardpairin
Affiliation:
Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand
P. Muangpat
Affiliation:
Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand
S. Sonpom
Affiliation:
Department of Agriculture Science, Faculty of Agriculture Natural Resources and Environment, Naresuan University, Phitsanulok, 65000, Thailand
A. Dumidae
Affiliation:
Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand
C. Subkrasae
Affiliation:
Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand
S. Tandhavanant
Affiliation:
Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok10400, Thailand
A. Thanwisai
Affiliation:
Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand Centre of Excellence in Medical Biotechnology (CEMB), Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand Center of Excellence for Biodiversity, Faculty of Sciences, Naresuan University, Phitsanulok, 65000Thailand
A. Vitta*
Affiliation:
Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand Centre of Excellence in Medical Biotechnology (CEMB), Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand Center of Excellence for Biodiversity, Faculty of Sciences, Naresuan University, Phitsanulok, 65000Thailand
*
Author for correspondence: Apichat Vitta, E-mail: apichatv@nu.ac.th
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Abstract

Entomopathogenic nematodes (EPNs) Steinernema and Heterorhabditis and their symbiotic bacteria, Xenorhabdus and Photorhabdus, have been successfully used for the control of insect pests. The objectives of this study were to survey the EPNs and symbiotic bacteria in the agricultural areas of the Phitsanulok province, Thailand, and to study the association between the soil parameters and presence of EPNs. We collected 200 soil samples from 40 soil sites in agricultural areas (field crops, horticulture crops and forest). The prevalence of EPNs was 8.0% (16/200). Fifteen of the EPN isolates were molecularly identified (based on 28S ribosomal DNA and internal transcribed spacer regions) as Steinernema siamkayai. Seven isolates of Xenorhabdus stockiae were identified using recombinase A sequencing. Phylogenetic analysis revealed that all the Steinernema and Xenorhabdus isolates were closely related to S. siamkayai (Indian strain) and X. stockiae (Thai strain), respectively. Significantly more EPNs were recovered from loam than from clay. Although the association between soil parameters (pH, temperature and moisture) and the presence of EPNs was not statistically significant, the elevation levels of the soil sites with and without EPNs were found to be different. Moreover, statistical comparisons between the agricultural areas revealed no significant differences. Therefore, we concluded that S. siamkayai is associated with X. stockiae in agricultural areas and that there is no association between the soil parameters of agricultural areas and presence of EPNs, except for soil texture and the elevation. Steinernema siamkayai may be applied as a biocontrol agent in agricultural areas.

Keywords

Agricultureentomopathogenic nematodesphylogenysymbiotic bacteria
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e192
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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