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An ELISA kit with two detection modes for the diagnosis of lymphatic filariasis

Published online by Cambridge University Press:  27 May 2014

S. Wongkamchai ,
W. Satimai ,
S. Loymek ,
H. Nochot  and
J.J. Boitano
S. Wongkamchai*
Affiliation:
Department of Parasitology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand
W. Satimai
Affiliation:
Bureau of Vector-Borne Disease, Department of Disease Control, Nonthaburi Province, Thailand
S. Loymek
Affiliation:
Filaria Project, Phikulthong Royal Development Study Center, Narathiwatt, Thailand
H. Nochot
Affiliation:
Department of Parasitology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand
J.J. Boitano
Affiliation:
Department of Parasitology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand
*
*Fax: +66-2-4112084 E-mail: sirichit.won@mahidol.ac.th
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Abstract

The aim of this study was to develop a low-cost antifilarial immunoglobulin (Ig) G4 detection kit for the diagnosis of lymphatic filariasis. The kit was designed to be used by minimally trained personnel without the constraints of expensive laboratory equipment. We provide a description of the development and validation of a single-serum-dilution based enzyme-linked immunosorbent assay (ELISA) kit with ready-to-use reagents for measuring antifilarial IgG4 antibodies. The kit was tested on residents in Brugia malayi-endemic areas in southern Thailand. Detection was performed by naked-eye observation of the resultant colour of the immunological reactivity. The coefficient of variation (CV) was used to assess the reproducibility of the results. Long-term stability was measured over a 6-month period. Sensitivity of the test kit was 97% when compared with microfilariae detection in thick blood smears. Specificity was 98.7% based on the sera of 57 patients living outside the endemic areas who were infected with other parasites and 100 parasite-free subjects. All positive CVs were < 10%. The test kit was remarkably stable over 6 months. Field validation was performed by the detection of antifilarial IgG4 in 4365 serum samples collected from residents of brugian filariasis-endemic areas and compared with outcome colours of the test samples by the naked eye. Subsequent ELISA evaluation of these results using an ELISA reader indicated high agreement by the kappa statistic. These results demonstrate that the test kit is efficient and useful for public health laboratories as an alternative tool for the diagnosis of lymphatic filarial infection.

Type
Research Papers
Information
Journal of Helminthology , Volume 89 , Issue 5 , September 2015 , pp. 552 - 558
Copyright
Copyright © Cambridge University Press 2014 

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References

Akobeng, A.K. (2007a) Understanding diagnostic tests 1: sensitivity, specificity and predictive values. Acta Paediatrica 96, 338–341.CrossRefGoogle ScholarPubMed
Akobeng, A.K. (2007b) Understanding diagnostic tests 3: receiver operating characteristic curves. Acta Paediatrica 96, 644–647.CrossRefGoogle ScholarPubMed
Engels, D. (2012) PC- and WHO's strategic plan for NTDs. Seventh meeting of GAELF, Washington, DC. Available athttp://www.filariasis.org/documents/allpresdayone.pdf (accessed July 2013).Google Scholar
GAELF (2012) Global Alliance to Eliminate Lymphatic Filariasis, history. Available athttp://www.filariasis.org/history.html (accessed July 2013).Google Scholar
Haarbrink, M., Terhell, A.J., Abadi, K., Asri, M., de Medeiros, F. & Yazdanbakhsh, M. (1999) Anti-filarial IgG4 in men and women living in Brugia malayi-endemic areas. Tropical Medicine and International Health 4, 93–97.CrossRefGoogle ScholarPubMed
Lammie, P.J. (2004) Research directly linked with GPLF activities (Operational Research). 2.1 Essential tools - diagnostics. American Journal of Tropical Medicine and Hygiene 71, 3–6.CrossRefGoogle Scholar
Lammie, P.J., Weil, G., Noordin, R., Kaliraj, P., Steel, C., Goodman, D., Lakshmikanthan, V.B. & Ottesen, E. (2004) Recombinant antigen-based antibody assays for the diagnosis and surveillance of lymphatic filariasis - a multicenter trial. Filaria Journal 3, 9.CrossRefGoogle ScholarPubMed
Landis, J.R. & Koch, G.G. (1977) The measurement of observer agreement for categorical data. Biometrics 33, 159–174.CrossRefGoogle ScholarPubMed
Molyneux, D.H. & Zagaria, N. (2002) Lymphatic filariasis elimination progress in global programme development. Annals of Tropical Medicine and Parasitology 96 (Suppl. 2), 15–40.CrossRefGoogle ScholarPubMed
Muck, A.E., Pires, M.L. & Lammie, P.J. (2003) Influence of infection with non-filarial helminths on the specificity of serological assays for antifilarial immunoglobulin G4. Transactions of the Royal Society of Tropical Medicine and Hygiene 97, 88–90.CrossRefGoogle ScholarPubMed
Ottesen, E.A. (2000) The Global Programme to Eliminate Lymphatic Filariasis. Tropical Medicine and International Health 5, 591–594.CrossRefGoogle ScholarPubMed
Rahmah, N., Shenoy, R.K., Nutman, T.B., Weiss, N., Gilmour, K., Maizels, R.M., Yazdanbakhsh, M. & Sartono, E. (2003) Multicentre laboratory evaluation of Brugia Rapid Dipstick test for detection of brugian filariasis. Tropical Medicine and International Health 8, 895–900.CrossRefGoogle ScholarPubMed
Servat, A., Feyssaguet, M., Blanchard, I., Morize, J.L., Schereffer, J.L., Boue, F. & Cliquet, F. (2007) A quantitative indirect ELISA to monitor the effectiveness of rabies vaccination in domestic and wild carnivores. Journal of Immunological Methods 318, 1–10.CrossRefGoogle ScholarPubMed
Viera, A.J. & Garrett, J.M. (2005) Understanding interobserver agreement: the kappa statistic. Family Medicine 37, 360–363.Google ScholarPubMed
Voller, A. & De Savigny, D. (1981) Diagnostic serology of tropical parasitic diseases. Journal of Immunological Methods 46, 1–29.CrossRefGoogle ScholarPubMed
Weil, G.J. (2005) Annex 6. Diagnostic tools for filariasis elimination programmes. Report of the Scientific Working Group on Lymphatic Filariasis. Available athttp://www.who.int/tdr/publications/publications/swg_lymph_fil.htm (accessed June 2013).Google Scholar
Weil, G.J., Lammie, P.J. & Weiss, N. (1997) The ICT filariasis test: a rapid-format antigen test for diagnosis of Bancroftian filariasis. Parasitology Today 13, 401–404.CrossRefGoogle Scholar
Wongkamchai, S., Rochjanawatsiriroj, C., Monkong, N., Nochot, H., Loymek, S., Jiraamornnimit, C., Hunnangkul, S. & Choochote, W. (2006) Diagnostic value of IgG isotype responses against Brugia malayi antifilarial antibodies in the clinical spectrum of brugian filariasis. Journal of Helminthology 80, 363–367.CrossRefGoogle ScholarPubMed