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Anthelmintic effects of Prosopis laevigatan-hexanic extract against Haemonchus contortus in artificially infected gerbils (Meriones unguiculatus)

Published online by Cambridge University Press:  21 July 2009

A.F. De Jesús-Gabino ,
P. Mendoza-de Gives ,
D.O. Salinas-Sánchez ,
M.E. López-Arellano ,
E. Liébano-Hernández ,
V.M. Hernández-Velázquez  and
G. Valladares-Cisneros
A.F. De Jesús-Gabino
Affiliation:
Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, INIFAP, Carr. Fed. Cuernavaca-Cuautla No. 8534, Col. Progreso, Jiutepec, MorelosCP 62550, México
P. Mendoza-de Gives*
Affiliation:
Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, INIFAP, Carr. Fed. Cuernavaca-Cuautla No. 8534, Col. Progreso, Jiutepec, MorelosCP 62550, México
D.O. Salinas-Sánchez
Affiliation:
FCB, de la UAEM, Av. Universidad 1001 Col. Chamilpa, Cuernavaca, MorelosCP 62209, México CEAMISH, de la UAEM, Av. Universidad 1001 Col. Chamilpa, Cuernavaca, MorelosCP 62209, México
M.E. López-Arellano
Affiliation:
Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, INIFAP, Carr. Fed. Cuernavaca-Cuautla No. 8534, Col. Progreso, Jiutepec, MorelosCP 62550, México
E. Liébano-Hernández
Affiliation:
Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, INIFAP, Carr. Fed. Cuernavaca-Cuautla No. 8534, Col. Progreso, Jiutepec, MorelosCP 62550, México
V.M. Hernández-Velázquez
Affiliation:
CEIB, de la UAEM, Av. Universidad 1001 Col. Chamilpa, Cuernavaca, MorelosCP 62209, México
G. Valladares-Cisneros
Affiliation:
FCQI, de la UAEM, Av. Universidad 1001 Col. Chamilpa, Cuernavaca, MorelosCP 62209, México
*
*Fax: (+52) 73 192850 Ext. 129 E-mail: pedromdgives@yahoo.com
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Abstract

The anthelmintic effect of Prosopis laevigata (mezquite) n-hexanic extract was evaluated against Haemonchus contortus endoparasitic stages in artificially infected gerbils (Meriones unguiculatus). Prosopis laevigata leaves were collected from the Sierra de Huautla, Ecological Reserve of the Biosphere, in Morelos State, Mexico; dehydrated under shade and macerated with n-hexane for 3 days, followed by distillation for 8 h. This procedure was repeated three times and the final extract was kept at 4°C. The in vivo effect of the plant extract was evaluated in gerbils artificially infected with H. contortus. Plant extract concentration was 40 mg/ml. Three groups of gerbils were as follows: group 1 (n = 7), P. laevigata extract at 100 μl intraperitoneally (IP); group 2 (n = 6), control – Tween 20 in water at a single dose of 100 μ l IP; group 3 (n = 8) also served as a control, receiving water only, to determine the mortality due to causes other than the plant extract. An additional group of seven gerbils (group 4) was administered fenbendazole, as a positive control. Five days later the animals were euthanized and stomach and mucosa removed to quantify the nematodes. Data were analysed using the Student's t-test to compare the mean of nematodes obtained in groups 1, 2 and 3. The parasite population in the plant extract treated group 1 was reduced by 42.5% (P < 0.05) with respect to the control group 2; and when control group 3 was used for comparison the parasitic reduction was estimated as 53.11%. This study shows the in vivo anthelmintic effect of P. laevigatan-hexane extract for the first time, using gerbils as an in vivo model, with potential use in sheep.

Type
Research Papers
Information
Journal of Helminthology , Volume 84 , Issue 1 , March 2010 , pp. 71 - 75
Copyright
Copyright © Cambridge University Press 2009

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References

Carretero-Accame, M.E. (2000) Plantas medicinales. Compuestos nitrogenados. Panorama actual Med. 24, 223–226.Google Scholar
Chandrawathani, P., Jamnah, O., Adnan, M., Waller, P.J., Larsen, M. & Gillespie, A.T. (2004) Field studies on the biological control of nematode parasites of sheep in the tropics, using the microfungus Duddingtonia flagrans. Veterinary Parasitology 120, 177–187.CrossRefGoogle ScholarPubMed
Cornelius-Jegede, O., Ologunja Ajanusi, J. & Rowland, I.S. (2006) Anthelmintic efficacy of extracts of Spigelia anthelmia Linn. on experimental Nippostrongylus braziliensis in rats. Journal of Veterinary Science 7, 229–232.CrossRefGoogle Scholar
Galicia-Aguilar, H.H., Mendoza de Gives, P., Salinas-Sánchez, D.O., López-Arellano, Ma.E., Liébano-Hernández, E., López-Aroche, U. & Valladares-Cisneros, G. (2008) In vitro nematocidal activity of plant extracts of the Mexican flora against Haemonchus contortus fourth larval stage. Annals of the New York Academy of Sciences 1149, 158–160.CrossRefGoogle Scholar
Githiori, J.B., Athanasiadou, S. & Thamsborg, S.M. (2006) Use of plants in novel approaches for control of gastrointestinal helminths in livestock with emphasis on small ruminants. Veterinary Parasitology 139, 308–320.CrossRefGoogle ScholarPubMed
Hernández, T., Canales, M.C., Caballero, J., Durán, A. & Lira, R. (2005) Análisis cuantitativo del conocimiento tradicional sobre plantas utilizadas para el tratamiento de enfermedades gastrointestinales en Zapotitlán de las Salinas, Puebla, México. Interciencia 30, 529–535.Google Scholar
Iqval, Z., Lqteef, M., Nisqr, K.N., Jqbbqr, A. & Shoqib, S. (2006) Anthemintic activity of Swertia chirata against gastrointestinal nematodes of sheep. Fitoterapia 77, 463–465.Google Scholar
Krychak-Furtado, S., Negrelle, R.B., Miguel, O.G., Zaniolo, S.R., Kapronezai, J., Ramos, S.J. & Sotello, A. (2005) Efeito de Carica papaya L. (Caricaceae) e Musa paradisiaca Linn. (Musaceae) sobre o desenvolvimento de ovos de nematódeos gastrintenstinais de ovino. Arquivos do Intituto Biologico (Sao Paulo) 72, 191–197.Google Scholar
Liébano, H.E. (2004) Identificación morfométrica de larvas infectantes de nematodos gastrointestinales y pulmonares en rumiantes domésticos de México. pp. 26–77in Prats, V.V. (Ed.) Diagnóstico y control de los nematodos gastrointestinales de los rumiantes de México. Libro Técnico No 1Jiutepec, Morelos, México, CENID-Parasitología Veterinaria del INIFAP.Google Scholar
López-Aroche, U., Salinas-Sánchez, D.O., Mendoza de Gives, P., López-Arellano, Ma.E., Liébano-Hernández, E., Valladares-Cisneros, G., Arias-Ataide, D.M. & Hernández-Velázquez, V. (2008) In vitro nematocidal effect of medicinal plants from ‘Sierra de Huautla’, Biosphere Reserve, Morelos, Mexico against Haemonchus contortus infective larvae. Journal of Helminthology 82, 25–31.CrossRefGoogle Scholar
López-Franco, Y.L., Goycochea, M., Valdéz, M.A. & Calderón de la Barca, A.M. (2006) Goma de Mezquite: una alternativa de uso industrial. Interciencia 31, 183–189.Google Scholar
Mendoza de Gives, P. & Herrera Rodríguez, D. (2004) Terapia antihelmíntica contra las nematodiasis gastrointestinales en rumiantes. pp. 115–128in Prats, V.V. (Ed.) Diagnóstico y control de los nematodos gastrointestinales de los rumiantes de México. Libro Técnico No 1. Jiutepec, Morelos, México, CENID-Parasitología Veterinaria del INIFAP.Google Scholar
Mendoza de Gives, P., Flores Crespo, J., Herrera Rodriguez, D., Vázquez Prats, V.M., Liébano Hernandez, E. & Ontiveros Fernandez, G.E. (1998) Biological control of Haemonchus contortus infective larvae in ovine faeces by administering an oral suspension of Duddingtonia flagrans chlamydospores to sheep. Journal of Helminthology 72, 343–347.CrossRefGoogle ScholarPubMed
Monroy-Ortiz, C. & Castillo, E.P. (2007) Plantas medicinales utilizadas en el Estado de Morelos. pp. 140–141. Centro de Investigaciones Biológicas, Universidad Autónoma del Estado de Morelos, Ciudad de Cuernavaca, Estado de Morelos, Mexico.Google Scholar
Ostlind, D.A., Cifelli, S., Mickle, W.G., Smith, S.K., Ewanciw, D.V., Rafalko, B., Felcetto, T. & Misura, A. (2006) Evaluation of broad-spectrum anthelmintic activity in a novel assay against Haemonchus contortus, Trichostrongylus colubriformis and T. sigmodontis in the gerbil Meriones unguiculatus. Journal of Helminthology 80, 393–396.CrossRefGoogle Scholar
Ramírez, V.G., López, A.M.E., Mendoza-de Gives, P., Liébano, H.E. & Vázquez, P.M. (2006) Desarrollo de nematodos endoparásitos de Haemonchus contortus in vitro como agente biológico de estudio. VII Congreso Nacional de Parasitología Veterinaria, Veracrúz, México.Google Scholar
Rzedowski, J. (1988) Vegetación de México. 432 pp. 1st edn.México D.F., Limusa.Google Scholar
Shaik, S.A., Terril, T.H., Miller, J.E., Kouakou, B., Kannan, G., Kaplan, R.M., Burke, J.M. & Mosjidis, J.A. (2006) Sericea lespedeza hay as a natural deworming agent against gastrointestinal nematode infection in goats. Veterinary Parasitology 139, 151–157.CrossRefGoogle Scholar
Síntesis Geográfica de Morelos, (1991) Coordinación de Servicios Nacionales de Estadística, Geografía e Informática (INEGI). México, D.F., Secretaría de Programación y Presupuesto.Google Scholar
Thiempont, D., Rochete, F. & Vanparijs, O.F.J. (1979) Diagnosing helminthiasis by coprological examination. 202 pp. 3rd edn.Beerse, Belgium, Jansen Research Foundation.Google Scholar
Valenzuela-Galván, D., Mariano, A.N., Osorio-Berinstain, M. & Urbina, F. (2006) First record of the sparkling-tailed hummingbird (Tilmatura dupontii, Trochilidae) for Sierra de Huautla Biosphere reserve, Morelos, Mexico. Huitzil 7, 15–17.CrossRefGoogle Scholar