Hostname: page-component-cd9895bd7-mkpzs Total loading time: 0 Render date: 2024-12-27T13:04:27.016Z Has data issue: false hasContentIssue false
  • English
  • Français

Description of a new species of the genus Anguillonema Fuchs, 1938 (Nematoda: Sphaerularioidea) with an identification key to the species

Published online by Cambridge University Press:  21 June 2018

A. Yaghoubi ,
E. Pourjam  and
M. Pedram
A. Yaghoubi
Affiliation:
Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
E. Pourjam*
Affiliation:
Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
M. Pedram
Affiliation:
Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
*
Author for correspondence: E. Pourjam, E-mail: Pourjame@modares.ac.ir
Get access Rights & Permissions [Opens in a new window]

Abstract

Anguillonema iranicum n. sp. is described and illustrated as the second species of this genus from Iran, based on morphological, morphometric and molecular characteristics. It is identified by a short, thin body, a continuous lip region, six lines on the lateral field, a short, thin stylet, a posteriorly located pharyngo-intestinal junction to excretory pore, the presence of a post-vulval uterine sac, vulval position at 89% (87.4–89.9%) of body length, an elongate conoid tail with a rounded to pointed tip and not dorsally bent, and common functional males with short spicules and lacking a bursa. Morphological differences between the new species and the three known species of the genus, namely A. amolensis, A. crenati and A. poligraphi, are discussed. Molecular phylogenetic studies of the new species using partial 18S rDNA sequence revealed that it formed a sister clade with three species of Howardula, one species of Anguillonema and one unidentified isolate. In phylogenetic analyses using partial sequences of 28S rDNA D2-D3 segment, the new species formed a clade with two isolates of Parasitylenchus. A key to identification of Anguillonema species is also presented.

Keywords

18S rDNA28S rDNA D2-D3Irannew speciesphylogenytaxonomy
Type
Research Paper
Information
Journal of Helminthology , Volume 93 , Issue 4 , July 2019 , pp. 504 - 512
Copyright
Copyright © Cambridge University Press 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Andrássy, I (2007) Free-living Nematodes of Hungary. Pedozoologica Hungarica No. 4. Budapest: Hungarian Natural History Museum.Google Scholar
Bastian, HC (1865) Monograph on the Anguillulidae, or free nematoids, marine, land, and freshwater; with descriptions of 100 new species. Transactions of the Linnean Society of London 25, 73–184.Google Scholar
Blinova, SL and Korentchenko, EA (1986) Phaenopsitylenchus laricis n. g., n. sp. (Nematoda: Phaenopsitylenchidae n. fam.) parasite of Phaenops guttulata and notes on the taxonomy of nematodes of the superfamily Sphaerularioidea. Trudy Gel'mintologicheskoy Laboratorii 34, 14–23.Google Scholar
Castresana, J (2000) Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Molecular Biology and Evolution 17, 540–552.Google Scholar
Cobb, NA (1921) Howardula benigna; a nema parasite of the cucumber-beetle (Diabrotica). Science 54, 667–670.Google Scholar
De Grisse, AT (1969) Redescription ou modifications de quelques techniques utilises dans l'étude des nématodes phytoparasitaires. Mededlingen Rijksfaculteit der Landbouwwetenschappen Gent 34, 351–369.Google Scholar
Ebsary, BA (1991) Catalog of the Order Tylenchida (Nematoda). Ottawa: Canadian Government Publishing Centre.Google Scholar
Edgar, RC (2004) MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32, 1792–1797.Google Scholar
Elsey, KD (1977) Howardula dominicki n. sp. infesting the tobacco flea beetle in North Carolina. Journal of Nematology 9, 338–342.Google Scholar
Fuchs, G (1938) Neue Parasitien und Halbparasiten bei Borkenkäfern und einige andere Nematoden. II., III. und IV. Teil. Zoologische Jahrbüucher (Systematik) 71, 123–190.Google Scholar
Geraert, E (2006) Functional and Detailed Morphology of the Tylenchida (Nematoda). Leiden: Brill Academic Publishers.Google Scholar
Goodey, T (1926) Hexatylus viviparus gen. et sp. nov., a nematode found in a diseased potato tuber. Journal of Helminthology 4, 27–30.Google Scholar
Holterman, M et al. (2006) Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown clades. Molecular Biology and Evolution 23, 1792–1800.Google Scholar
Husain, SI and Khan, AM (1968) Paurodontella n. gen. and three new species of nematodes from North India (Nematoda: Neotylenchidae). Nematologica 13, 493–500.Google Scholar
Jairajpuri, MS and Siddiqi, MR (1969) Paurodontoides n. gen. (Paurodontidae) with an outline classification of Neotylenchoidea n. rank. Nematologica 15, 287–288.Google Scholar
Kanzaki, N and Futai, K (2002) A PCR primer set for determination of phylogenetic relationships of Bursaphelenchus species within the xylophilus-group. Nematology 4, 35–41.Google Scholar
Koshel, EI et al. (2014) Phylogenetic analysis of entomoparasitic nematodes, potential control agents of flea populations in natural foci of plague. BioMed Research International 2014, 135218.Google Scholar
Larget, B and Simon, DL (1999) Markov chain Monte Carlo algorithms for the Bayesian analysis of phylogenetic trees. Molecular Biology and Evolution 16, 750–759.Google Scholar
Leuckart, R (1884) Über einen neuen heterogenen Nematoden. Verhandlungen der Gesellschaft Deutscher Naturforscher und Ärzte 57, 320.Google Scholar
Lubbock, J (1861) On Sphaerularia bombi. Natural History Review 1, 44–57.Google Scholar
Massey, CL (1974) Biology and Taxonomy of Nematode Parasites and Associates of Bark Beetles in the United States. Agriculture Handbook No. 446. Washington, DC: USDA Forest Service.Google Scholar
Micoletzky, H (1922) Die freilebenden Erd-Nematoden. Archive fur Naturgeschichte Berlin Abt 87, 1–650.Google Scholar
Mobasseri, M, Pedram, M and Pourjam, E (2017) A new species of the rare genus Anguillonema Fuchs, 1938 (Nematoda: Hexatylina, Sphaerularioidea) with its molecular phylogenetic study. Journal of Nematology 49, 286–294.Google Scholar
Nunn, GB (1992) Nematode Molecular Evolution (Doctoral dissertation). University of Nottingham, UK.Google Scholar
Nylander, JAA (2004) MrModeltest v2. Evolutionary Biology Centre, Uppsala University. Available at http://www.ebc.uu.se/systzoo/staff/nylander.html.Google Scholar
Oldham, JN (1933) On Howardula phyllotretae n. sp., a nematode parasite of flea beetles (Chrysomelidae: Coleoptera), with some observations on its incidence. Journal of Helminthology 11, 119–136.Google Scholar
Pereira, C (1931) Myenchus botelhoi n. sp., curiosa nematoide parasite de Limnobdella brasiliensis Pinto (Hirudinea). Thesis. Universidade de São Paulo, Brazil.Google Scholar
Poinar, GO (1975) Entomogenous Nematodes: A Manual and Host List of Insect–Nematode Associations. Leiden: Brill.Google Scholar
Rambaut, A and Drummond, AJ (2009) Tracer version 1.5. Available at http://beast.bio.ed.ac.uk.Google Scholar
Ronquist, F and Huelsenbeck, JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572–1574.Google Scholar
Rühm, W (1955) Über einige an holzbrütende Ipiden gebundene Nematodenarten. Zoologischer Anzeiger 155, 70–83.Google Scholar
Rühm, W (1956) Die Nematoden der Ipiden. Parasitologische Schriftenreihe 6, 1–437.Google Scholar
Siddiqi, MR (1980) The origin and phylogeny of the orders Tylenchida Thorne, 1949 and Aphelenchida n. ord. Helminthological Abstracts Series B 49, 143–170.Google Scholar
Siddiqi, MR (1986) Tylenchida: Parasites of Plants and Insects. London: Commonwealth Institute of Parasitology of the Commonwealth Agricultural Bureau.Google Scholar
Siddiqi, MR (2000) Tylenchida: Parasites of Plants and Insects, 2nd Edn. Wallingford: CABI Publishing.Google Scholar
Silvestro, D and Michalak, I (2011) raxmlGUI: a graphical front-end for RAxML. Organisms Diversity and Evolution 12, 335–337.Google Scholar
Slobodyanyuk, OV (1986) Revision of Heterotylenchus and erection of Wachekitylenchus n. g. (Allantonematidae, Tylenchida). Parazitologiya 20, 466–475.Google Scholar
Slobodyanyuk, OV (1991) Validation of the genus Rubzovinema gen. n. (Sphaerularioidea) and revision of Rubzovinema ceratophylla comb. n. parasite of Citellophilus tesquorum. Zoologichesky Zhurnal 70, 33–43.Google Scholar
Steiner, G (1931) Neotylenchus abulbosus n. g., n. sp. (Tylenchidae, Nematoda), the causal agent of a new nematosis of various crop plants. Journal of the Washington Academy of Sciences 21, 536–538.Google Scholar
Strassen, OKL (1892) Bradynema rigidum.v. Sieb. Zeitschrift für wissenschaftliche Zoologie 54, 655–747.Google Scholar
Sumenkova, NI (1989) Nematodes of Plants and Soils: Neotylenchoidea. Leiden: Brill.Google Scholar
Tamura, K et al. (2013) MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30, 2725–2729.Google Scholar
Taylor, DP and Pillai, JK (1967) Paraphelenchus acontioides n. sp. (Nematoda: Paraphelenchidae), a mycophagous nematode from Illinois, with observations on its feeding habits and a key to the species of Paraphelenchus. Proceedings of the Helminthological Society of Washington 34, 51–54.Google Scholar
Thorne, G (1941) Some nematodes of the family Tylenchidae which do not possess a valvular median esophageal bulb. Great Basin Naturalist 2, 37–85.Google Scholar
Whitehead, AG and Hemming, JR (1965) A comparison of some quantitative methods of extracting small vermiform nematodes from soil. Annals of Applied Biology 55, 25–38.Google Scholar
Yaghoubi, A, Pourjam, E and Pedram, M (2018) Paurodontella gilanica n. sp. (Nematoda: Sphaerularioidea) from Iran. Nematology 20, 471–482.Google Scholar
Yaghoubi, A et al. (2014) Description of Abursanema iranicum n. gen., n. sp. (Nematoda: Hexatylina, Sphaerularioidea) from Iran and its phylogenetic relationships. Zootaxa 3826, 301–314.Google Scholar