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Description of Rotylenchus zhongshanensis sp. nov. (Tylenchomorpha: Hoplolaimidae) and discovery of its endosymbiont Cardinium

Published online by Cambridge University Press:  20 July 2022

F. Guo ,
P. Castillo ,
C. Li ,
X. Qing Open the ORCID record for X. Qing [Opens in a new window]  and
H. Li
F. Guo
Affiliation:
Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, People's Republic of China
P. Castillo
Affiliation:
Spanish National Research Council (CSIC), Institute for Sustainable Agriculture (IAS), Campus de Excelencia Internacional Agrolimentario, ceiA3, Avenida Menendez Pidal s/n, 14004 Cordoba, Spain
C. Li
Affiliation:
Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China
X. Qing*
Affiliation:
Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, People's Republic of China
H. Li
Affiliation:
Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, People's Republic of China
*
Author for correspondence: Xue Qing, E-mail: qingxue@njau.edu.cn
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Abstract

A new bisexual species of Rotylenchus is described and illustrated based on morphological, morphometric and molecular characterizations. Rotylenchus zhongshanensis sp. nov. is characterized by having a conoid lip region complying with the basic pattern for Hoplolaimidae, but with pharyngeal glands slightly overlapping intestine dorsally and cuticle thickened abnormally in female tail terminus. Females have robust stylet (30.1–33.8 μm). The pharyngeal gland has short dorsal (11.2–16.8 μm) overlap on the intestine. The vulva is located at 48.0–56.5% of body length, and phasmids are pore-like, 4–6 annuli posterior to the anus. For males, phasmids are pore-like, 11–17 annuli posterior to cloaca. The spicules are ventrally arcuate (21.0–28.5 μm) with gubernaculum in 5–8 μm length. The rRNA and mitochondrial COI genes were successfully sequenced from the assembled whole-genome sequences of the new species, and were used for reconstructing the phylogenetic relationships of the new species. A new strain of cyto-endosymbiont Cardinium was also discovered from the genome sequences of R. zhongshanensis sp. nov. The 16S rRNA phylogeny analyses revealed that this new bacterial strain is closed to that from cyst and root-lesion nematodes.

Keywords

Bacterial cyto-endosymbiontgenome assemblymolecularmorphologynew speciesphylogenyplant-parasitic nematodetaxonomy
Type
Research Paper
Information
Journal of Helminthology , Volume 96 , 2022 , e48
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

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