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Development of Microphalloides japonicus (Osborn, 1919) metacercariae in vitro (Trematoda: Microphallidae)

Published online by Cambridge University Press:  05 June 2009

Takahiro Fujino ,
Fusanori Hamajima ,
Yoichi Ishii  and
Ryoichi Mori
Takahiro Fujino
Affiliation:
Department of Parasitology, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan
Fusanori Hamajima
Affiliation:
Department of Parasitology, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan
Yoichi Ishii
Affiliation:
Department of Parasitology, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan
Ryoichi Mori
Affiliation:
Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan
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Abstract

Metacercariae of the trematode Microphalloides japonicus (Osborn, 1919) were cultivated in various media to gravid adults, and the results were compared with those in vivo. A few eggs were produced in the uterus even in balanced salt solution, although cultivation in Eagle's MEM and NCTC 109 media was much better. Addition of heat-inactivated serum resulted in even better development. Encysted metacercariae also produced eggs in some media, but most of them were incomplete and without shells.

Type
Research Article
Information
Journal of Helminthology , Volume 51 , Issue 2 , June 1977 , pp. 125 - 129
Copyright
Copyright © Cambridge University Press 1977

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References

REFERENCES

Asanji, M. F. and Williams, M. O. (1975) Studies on the excystment of trematode metacercariae in vitro. Zeitschrift fiir Parasitenkunde, 47, 151–163.CrossRefGoogle ScholarPubMed
Basch, P. F., Diconza, J. J. and Johnson, B. E. (1973) Strigeid trematodes (Cotylurus lutzi) cultured in vitro: Production of normal eggs with continuance of life cycle. Journal of Parasitology, 59, 319–322.CrossRefGoogle ScholarPubMed
Hunter, W. S. and Chait, D. C. (1952) Notes on excystment and culture in vitro of the microphallid trematode Gynaecotyle adunca. Journal of Parasitology, 38, 87.CrossRefGoogle Scholar
Kannangara, D. W. W. (1972) On some aspects of the parasites of Ceylonese fresh water crabs. Ceylon Journal of Sciences, 10, 32–38.Google Scholar
Kannangara, D. W. W. and Smyth, J. D. (1974) In vitro cultivation of Diplostomum spathaceum and D. phoxini metacercariae. International Journal for Parasitology, 4, 667–673.CrossRefGoogle Scholar
Miyazaki, I. (1938) On a new trematode species whose intermediate host is a crab, Helice tridens tridens (de Haan). Tokio Izi Sinsi, 3074, 638–641.Google Scholar
Miyazaki, I. and Nishimura, H. (1943) On the natural final host of the trematode, Microphalloides japonicus (Osborn, 1919) Yoshida, 1938. Hukuoka Acta Medica, 36 (5), 1–4.Google Scholar
Smyth, J. D. (1966) The physiology of trematodes. W. H. Freeman and Co: San Francisco.Google Scholar
Voge, M. and Jeong, K. (1971) Growth in vitro of Cotylurus lutzi Basch 1969 (Tremadota: Strigeidae) from tetracotyle to patent adult. International Journal for Parasitology, 1, 139–143.CrossRefGoogle ScholarPubMed
Yasuraoka, K., Kaiho, M., Hata, H. and Endo, T. (1974) Growth in vitro of Parvatrema timondavidi Bartoli 1963 (Trematoda: Gymnophallidae) from the metacercarial stage to egg production. Parasitology, 68, 293–320.CrossRefGoogle ScholarPubMed