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Experimental infection of larval Echinococcus multilocularis in the rodent brain as a model for cerebral alveolar echinococcosis

Published online by Cambridge University Press:  05 June 2009

Y. Sato ,
M. Nakao ,
K. Nakaya  and
A. Ito
Y. Sato*
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa 078, Japan
M. Nakao
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa 078, Japan
K. Nakaya
Affiliation:
Animal Laboratory for Medical Research, Asahikawa Medical College, Asahikawa 078, Japan
A. Ito
Affiliation:
Deparment of Parasitology, Gifu University School of Medicine, Gifu 500, Japan
*
* Fax: +81 166 68 2429 E-mail: yukita@asahikawa-med.ac.jp
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Abstract

Experimental infection of larval Echinococcus multilocularis in the rodent brain was attempted to establish a murine model for cerebral alveolar echinococcosis. Balb/c mice and jirds were injected intracranially with 10% of a homogenated hydatid cyst mass. Small cystic larvae were observed macroscopically in the cranial cavity 1, 2 and 5 months post-infection in both mice and jirds. Some larval cysts from both rodents contained mature or immature protoscoleces. In mice, the laminated layer was found in the lateral ventricle 2 months post-infection but without protoscoleces. At five months post-infection, larger larval cysts were found in the cranial cavity of a mouse, which also demonstrated partial palsy of the legs. A laminated layer with mature protoscoleces was observed in the third ventricle and the mouse also harboured, in the left lung, a larval cyst containing protoscoleces surrounded by lymphocytes. Jirds were also found to be infected with metacestodes in the cranial cavity, but neither unusual behaviour nor establishment of cysts inside the brain was observed in jirds during the course of infection.

Type
Research Papers
Information
Journal of Helminthology , Volume 72 , Issue 1 , March 1998 , pp. 59 - 64
Copyright
Copyright © Cambridge University Press 1998

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References

references

Ali-Khan, Z., Jothy, S. & Al-Karmi, T. (1983) Murine alveolar hydatidosis: a potential experimental model for the study of AA-amyloidosis. British Journal of Experimental Pathology 64, 599–611.Google Scholar
Altinörs, N., Kars, Z., Cepoglu, C., Gürses, L., Sagbil, S. & Ariyürek, M. (1991) CT findings and surgical treatment of double intracranial echinococcal cysts. Infection 19, 110–113.Google Scholar
Ammann, R. & Eckert, J. (1995) Clinical diagnosis and treatment of echinococcosis in humans. pp. 411–463 in Thompson, R.C.A. & Lymbery, A.J. (Eds) Echinococcus and hydatid disease. Wallingford, CAB International.Google Scholar
Eckert, J., Thompson, R.C.A. & Mehlhorn, H. (1983) Proliferation and metastases formation of larval Echinococcus multilocularis: I. Zeitschrift für Parasitenkunde 69, 737–748.CrossRefGoogle ScholarPubMed
Gemmell, M.A., Matyas, Z., Pawlowski, Z. & Soulsby, E.J.L. (1983) Guidelines for surveillance, prevention and control of taeniasis/cysticercosis, VPH/83. 49, 207 pp., World Health Organization, Geneva.Google Scholar
Ihara, T., Imai, T., Saito, H., Tashiro, K. & Onmura, Y. (1991) Cerebral alveolar hydatid cyst: case report. Neurologia Medico-Chirurgica 31, 342–345.CrossRefGoogle ScholarPubMed
Kondo, H., Wada, Y., Bando, G., Kosuge, M., Yagi, K. & Oku, Y. (1996) Alveolar hydatidosis in a gorilla and a ring-tailed lemur in Japan. Journal of Veterinary Medical Sciences 58, 447–449.Google Scholar
Liance, M., Vuitton, D.A., Guerret-Stocker, S., Carbillet, J.P., Grimaud, J.A. & Houin, R. (1984) Experimental alveolar echinococcosis, Suitability of a murine model of intrahepatic infection by Echinococcus multilocularis for immunological studies. Experientia 40, 1436–1439.CrossRefGoogle ScholarPubMed
Mahajan, R.C. (1983) Geographical distribution of human cysticercosis. pp. 39–46 in Flisser, A., Willms, K., Laclette, J.P. & Larralde, C. (Eds) Cysticercosis: present state of knowledge and perspectives. Academic Press.Google Scholar
Matsuhisa, T. (1996) The mechanism of distant metastases of alveolar hydatid disease. Hokkaido Journal of Medical Sciences 71, 369–376 (in Japanese with English summary).Google ScholarPubMed
Mehlhorn, H., Eckert, J. & Thompson, R.C.A. (1983) Proliferation and metastases formation of larval Echinococcus multilocularis: II. Zeitschrift fiir Parasitenkunde 69, 749–763.Google Scholar
Nakao, M., Nakaya, K. & Kutsumi, H. (1990) Murine model for hepatic alveolar hydatid disease without biohazard. Japanese Journal of Parasitology 39, 296–298.Google Scholar
Nakaya, K., Nakao, M. & Ito, A. (1997) Echinococcus multilocularis: mouse strain difference in hydatid development. Journal of Helminthology 71, 53–56.Google Scholar
Ohbayashi, M., Rausch, R.L. & Fay, F.H. (1971) On the ecology and distribution of Echinococcus spp. (Cestoda: Taeniidae), and characteristics of their development in the intermediate host. II. Comparative studies on the larval E. multilocularis Leuckart, 1863, in the intermediate host. Japanese Journal of Veterinary Research 19, Suppl. 3, 1–53.Google Scholar
Ohnishi, K. (1984) Trans portal, secondary hepatic alveolar echinococcosis of rats. Journal of Parasitology 70, 987–988.CrossRefGoogle ScholarPubMed
Sato, N., Matsushita, M. & Uchino, J. (1993) Clinical features, pp. 63–68 inUchino, J. & Sato, N. (Eds) Alveolar echinococcosis of the liver. Hokkaido University School of Medicine.Google Scholar
Schantz, P.M., Moore, A.C., Munoz, J.L., Hartman, B.J., Schefer, J.A., Aron, A.M., Persaud, D., Sarti, E., Wilson, M. & Flisser, A. (1992) Neurocysticercosis in an orthodox Jewish community in New York City. New England Journal of Medicine 327, 692–695.CrossRefGoogle Scholar
Schantz, P.M., Chai, J., Craig, P.S., Eckert, J., Jenkins, D.J., Macpherson, C.N.L. & Thakur, A. (1995) Epidemiology and control of hydatid disease, pp. 233–331 in Thompson, R.C.A. & Lymbery, A.J. (Eds) Echinococcus and hydatid disease. Wallingford, CAB International.Google Scholar
Yamashita, J., Ohbayashi, M., Kitamura, Y., Suzuki, K. & Okugi, M. (1958) Studies on echinococcosis. VIII. Experimental echinococcosis multilocularis in various rodents; especially on the difference of susceptibility among uniform strains of the mouse. Japanese Journal of Veterinary Research 6, 135–155.Google Scholar
Yamashita, J., Ohbayashi, M. & Doi, R. (1963) Studies on echinococcosis. XV. Secondary multilocular echinococcosis by intrahepatic inoculation. Japanese Journal of Veterinary Research 11, 55–60.Google Scholar