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Freeze fixation-dehydration as a method of preparation of Gyrodactylus (Monogenea) for SEM

Published online by Cambridge University Press:  05 June 2009

C.J. Veltkamp ,
G.R. Richards  and
J.C. Chubb
C.J. Veltkamp
Affiliation:
Department of Environmental and Evolutionary Biology, The University of Liverpool, Derby Building, Liverpool, L69 3BX, UK
G.R. Richards
Affiliation:
Department of Environmental and Evolutionary Biology, The University of Liverpool, Derby Building, Liverpool, L69 3BX, UK
J.C. Chubb
Affiliation:
Department of Environmental and Evolutionary Biology, The University of Liverpool, Derby Building, Liverpool, L69 3BX, UK
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Abstract

Freeze fixation-dehydration was used for the first time in the preparation of attached Gyrodactylus for SEM viewing. The technique provided instant immobilization of specimens before death and negligible shrinkage throughout the fixation-dehydration process. Comparisons of sample means of two linear measurements of attached opisthaptors showed 20% more shrinkage of Gyrodactylus fixed using 10% neutral buffered formalin than those which were freeze fixed. Freeze fixation-dehydration was excellent for the study of gross external morphology of attached Gyrodactylus. However, the freeze fixation-dehydration process may cause disruption of intracellular structural components making delicate tissues brittle and more prone to damage during subsequent manipulation.

Type
Research Papers
Information
Journal of Helminthology , Volume 70 , Issue 1 , March 1996 , pp. 85 - 89
Copyright
Copyright © Cambridge University Press 1996

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