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Genomic variability within laboratory and wild subspecies of Heligmosomoides polygyrus

Published online by Cambridge University Press:  05 June 2009

M.A. Abu-Madi ,
A.P. Reid ,
J.W. Lewis  and
W.M. Hominick
M.A. Abu-Madi
Affiliation:
Department of Biology, Royal Holloway, University of London, Egham, Surrey, TW20 OEX, UK
A.P. Reid
Affiliation:
Imperial College of Science, Technology and Medicine, Department of Biology, Silwood Park, Ascot, Berks, SL5 7PY, UK
J.W. Lewis
Affiliation:
Department of Biology, Royal Holloway, University of London, Egham, Surrey, TW20 OEX, UK
W.M. Hominick
Affiliation:
Imperial College of Science, Technology and Medicine, Department of Biology, Silwood Park, Ascot, Berks, SL5 7PY, UK
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Abstract

Genomic DNA extracted from laboratory and wild subspecies of the trichostrongyle nematode Heligmosomoides polygyrus were compared using RFLP and DNA/DNA hybridization techniques. Eight restriction endonuclease digests of the genomic DNA of the two subspecies were hybridized with heterologous ribosomal DNA probes and the total radio-isotope labelled DNA of the laboratory subspecies. DNA hybridization of the two subspecies of H. polygyrus yielded different banding patterns when probed with the rDNA clones in Pvu II digests and when total genomic DNA was used as the probe in Hind III and Pvu II digests. The remaining hybridization profiles of both subspecies were identical.

Type
Research Papers
Information
Journal of Helminthology , Volume 68 , Issue 2 , June 1994 , pp. 93 - 96
Copyright
Copyright © Cambridge University Press 1994

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