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Histochemical localization and functional significance of some enzymes in various tissues of Ceylonocotyle scoliocoelium cultured in vitro

Published online by Cambridge University Press:  05 June 2009

A. N. Sharma
A. N. Sharma
Affiliation:
Department of Zoology, University of Udaipur, Udaipur 313 001, India
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Abstract

TPase and SDII activities were studied in various tissues of Ceylonocotyle scoliocoelium maintained in vitro in medium A for 15 days, using standard histochemical techniques. Results were compared with the normal activities of these enzymes in the tissues of worms freshly collected. Low ATPase and SDH enzyme activities in the tissues of cultured flukes indicated their low metabolic rate. Intensity of enzymatic reactions was also found to be tissue specific. The suitability of culture medium ‘A’ for the in vitro cultivation of amphistomes is discussed.

Type
Research Article
Information
Journal of Helminthology , Volume 58 , Issue 3 , September 1984 , pp. 201 - 206
Copyright
Copyright © Cambridge University Press 1984

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References

REFERENCES

Butler, M. & Fried, B. (1977) Histochcmical and thin layer chromatographic analysis of neutral lipids in Echinostoma rcvolutum mctaccrcariac cultured in vitro. Journal of Parasitology, 63, 1041–1045.CrossRefGoogle ScholarPubMed
Nachlas, M.M., Tsou, K.C., Desouza, E., Cheng, C.S. & Seliyman, A.M. (1957) Cytochcmical demonstration of succinic dehydrogenase by the use of a new p-dinitrophenyl substituted ditctrazolc. Journal of Hlstochcmistry and Cytochemistry, 5, 420–436.CrossRefGoogle Scholar
Padykula, H.A. & Herman, E. (1955) The specificity of the histochcmical method for adenosinc triphosphatasc. Journal of Histochemistry and Cytochemistry, 3, 170–195.CrossRefGoogle Scholar
Patil, H.S. & Rodgi, S.S. (1976) Histochcmical localization of non-specific cstcrasc activity in Paramphistomum cervi (Trcmatoda: Paramphistomidac). Current Science, 45, 625–626.Google Scholar
Rohrbacher, G.H. (1957) Observation on the survival in vitro of bacteria free adult common liver fluke Fasciola hepatica. Journal of Parasitology, 43, 9–18.CrossRefGoogle Scholar
Roy, T.K. (1980) Distribution and functional significance of phosphatascs in the Bovine amphistomc Ceylonocotyle scoliocoelium. Indian Journal of Experimental Biology, 18, 385–392.Google ScholarPubMed
Sharma, P.N. (1977) Histochcmical distribution and functional significance of alkaline phosphatasc, add phosphatasc, ATPasc and 5'Nuclcotidasc in the gut of intestinal trematodes. Rivista di Parasitologia, 38, 2–3.Google Scholar
Sharma, P.N. (1978) Histochcmical localization of succinate dehydrogenase in the lymphatic system of a trcmatodc Ceylonocotylc scoliocoelium. Journal of Hclminthology, 52, 159–162.Google Scholar
Sharma, A.N. & Sharma, P.N. (1980) Chcmoarchilcctural characteristics of germ cells during spermatogencsis in Ceylonocotylc scoliocoelium (Trcmatoda: Digcnca). Indian Journal of Experimental Biology, 18, 1282–1287.Google Scholar
Sharma, P.N., Mandawat, S. & Sharma, A.N. (1981) Cytochemistry of Mchlis gland in C. scoliocoelium. Journal of Helminthology, 55, 141–148.CrossRefGoogle Scholar
Sharma, A.N. & Sharma, P.N. (1982) Preliminary experiments on the in vitro cultivation of an amphistomc Ceylonocolyle scoliocoelium (Trcmatoda: Digcnca). Journal of Hclminthology, 56, 169–176.CrossRefGoogle Scholar