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Immunogenic activity of the fish tapeworm Pterobothrium heteracanthum (Trypanorhyncha: Pterobothriidae) in BALB/c mice

Published online by Cambridge University Press:  03 December 2013

D.P.B.G. Mattos ,
M.A. Verícimo ,
L.M.S. Lopes  and
S.C. São Clemente
D.P.B.G. Mattos*
Affiliation:
Programa de Pós-graduação em Medicina Veterinária, Faculdade de Medicina Veterinária, Universidade Federal Fluminense, Vital Brazil 64, Santa Rosa, Niterói - RJ 2423-340, Brazil
M.A. Verícimo
Affiliation:
Departamento de Imunobiologia, Instituto de Biologia, Universidade Federal Fluminense, Outeiro São João Batista, Centro, Niterói - RJ 24210-150, Brazil
L.M.S. Lopes
Affiliation:
Laboratório de Inspeção e Tecnologia de Pescado, Faculdade de Veterinária, Universidade Federal Fluminense, Vital Brazil 64, Santa Rosa, Niterói - RJ 24230-340, Brazil
S.C. São Clemente
Affiliation:
Programa de Pós-graduação em Medicina Veterinária, Faculdade de Medicina Veterinária, Universidade Federal Fluminense, Vital Brazil 64, Santa Rosa, Niterói - RJ 2423-340, Brazil
*
*E-mail: danuzamattos@vm.uff.br
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Abstract

The aim of this study was to verify the immunogenicity of Pterobothrium heteracanthum (Cestoda: Trypanorhyncha) crude protein extract (PH-CPE) in BALB/c mice. The parasites were obtained from Micropogonias furnieri (Osteichthyes: Sciaenidae). Groups of six mice were each immunized with 10, 50 or 100 μg of PH-CPE, on days 0 and 35. Both specific IgG and IgE responses were developed after immunization. The immunoblot assay revealed that specific IgG recognizes PH-CPE proteins with two molecular weight ranges, 60–75 and 30–40 kDa, and that IgE recognizes larger proteins over 120 kDa. This appears to be the first report on the immunogenicity of metacestodes within the Pterobothriidae and that PH-CPE is a potential inducer of a specific IgE response.

Type
Research Papers
Information
Journal of Helminthology , Volume 89 , Issue 2 , March 2015 , pp. 203 - 207
Copyright
Copyright © Cambridge University Press 2013 

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References

Alves, D.R. & Luque, J.L. (2001) Community ecology of the metazoan parasites of white croaker, Micropogonias furnieri (Osteichthyes: Sciaenidae), from the coastal zone of the state of Rio de Janeiro, Brazil. Memórias do Instituto Oswaldo Cruz 96, 145–153.CrossRefGoogle ScholarPubMed
Al-Zubaidyl, A.B. & Mhaisen, F.T. (2011) Larval tapeworms (Cestoda: Trypanorhyncha) from some Red Sea fishes, Yemen. Mesopotamian Journal of Marine Science 26, 1–14.Google Scholar
Antunes, D.M.F., Costa, J.P., Campos, S.M.N., Paschoal, P.O., Garrido, V., Siqueira, M., Teixeira, G.A.P.B. & Cardoso, G.P. (2009) The serum d-xylose test as a useful tool to identify malabsorption in rats with antigen specific gut inflammatory reaction. International Journal of Experimental Pathology 90, 141–147.Google Scholar
Audicana, M.T. & Kennedy, M.W. (2008) Anisakis simplex: from obscure infectious worm to inducer of immune hypersensitivity. Clinical Microbiology Reviews 21, 360–379.CrossRefGoogle ScholarPubMed
Audicana, M.T., Ansotegui, I.J., Fernández de Corres, L. & Kennedy, M.W. (2002) Anisakis simplex: dangerous dead and alive? Trends in Parasitology 18, 20–25.Google Scholar
Broglia, A. & Kapel, C. (2011) Changing dietary habits in a changing world: Emerging drivers for the transmission of foodborne parasitic zoonoses. Veterinary Parasitology 182, 2–13.Google Scholar
Campbell, R.A. & Beveridge, I. (1996) Revision of the Family Pterobothriidae Pintner, 1931 (Cestoda: Trypanorhyncha). Invertebrate Taxonomy 10, 617–662.CrossRefGoogle Scholar
Daschner, A., Cuéllar, C. & Rodero, M. (2012) The Anisakis allergy debate: does an evolutionary approach help? Trends in Parasitology 28, 9–15.CrossRefGoogle ScholarPubMed
Dearman, R.J. & Kimber, I. (2001) Determination of protein allergenicity: studies in mice. Toxicology Letters 120, 181–186.Google Scholar
Fernández de Corres, L., Audicana, M., Del Pozo, M.D., Muñoz, L., Fernández, L., Navarro, J.A., García, M. & Diez, J. (1996) Anisakis simplex induces not only anisakiasis: report on 28 cases of allergy caused by this nematode. Journal of Investigational Allergology and Clinical Immunology 6, 315–319.Google Scholar
Fonseca, M.C.G., São Clemente, S.C., Felizardo, N.N., Gomes, D.C. & Knoff, M. (2012) Trypanorhyncha cestodes of hygienic-sanitary importance infecting flounders Paralichthys patagonicus Jordan, 1889 and Xystreurys rasile (Jordan, 1891) of the Neotropical region, Brazil. Parasitology Research 111, 865–874.Google Scholar
Fripp, P.J. & Mason, P.R. (1983) Spurious human infection with a Trypanorhynchiid tapeworm. South African Journal of Science 79, 473.Google Scholar
Gòmez-Morales, M.A., Ludovisi, A., Giuffra, E., Manfredi, M.T., Piccolo, G. & Pozio, E. (2008) Allergenic activity of Molicola horridus (Cestoda, Trypanorhyncha), a cosmopolitan fish parasite, in a mouse model. Veterinary Parasitology 157, 314–320.Google Scholar
Haseli, M., Malek, M., Valinasab, T. & Palm, H.W. (2011) Trypanorhynch cestodes of teleost fish from the Persian Gulf, Iran. Journal of Helminthology 85, 215–224.Google Scholar
Heinz, H.J. (1954) A case of tetrarhynchid (cestode) infection in man. Revista Ecuatoriana de Entomología y Parasitología 2, 227–230.Google Scholar
Kikuchi, Y., Takenouchi, T., Kamiya, M. & Ozaki, H. (1981) Trypanorhynchid cestode larva found on the human palatine tonsil. Japanese Journal of Parasitology 30, 497–499.Google Scholar
Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (5259), 680–685.Google Scholar
Lowry, O.H., Rosebrough, N.J., Farr, A.L. & Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–275.Google Scholar
Martínez de Velasco, G., Rodero, M., Zapatero, L. & Cuéllar, C. (2002) Humoral immune responses induced by Kudoa sp. (Myxosporea: Multivalvulida) antigens in BALB/c mice. Memórias do Instituto Oswaldo Cruz 97, 1091–1095.Google Scholar
Moore, B.R., Buckworth, R.C., Moss, H. & Lester, R.J.G. (2003) Stock discrimination and movements of narrow-barred Spanish mackerel across northern Australia as indicated by parasites. Journal of Fish Biology 63, 765–779.CrossRefGoogle Scholar
Overstreet, R.M. (1977) Poecilancistrium caryophyllum and other trypanorhynch cestode plerocercoids from the musculature of Cynoscion nebulosus and other Sciaenid fishes in the Gulf of Mexico. Journal of Parasitology 63, 780–789.Google Scholar
Overstreet, R.M. (1978) Trypanorhynch infections in the flesh of sciaenid fishes. Marine Fisheries Review 40, 37–38.Google Scholar
Palm, H.W. (1997) Trypanorhynch cestodes of commercial fishes from northeast Brazilian coastal waters. Memórias do Instituto Oswaldo Cruz 92, 69–79.CrossRefGoogle Scholar
Palm, H., Möller, H. & Petersen, F. (1993) Otobothrium penetrans (Cestoda; Trypanorhyncha) in the flesh of belonid fish from Philippine waters. International Journal of Parasitology 23, 749–755.Google Scholar
Paschoal, P.O., Campos, S.M.N., Pedruzzi, M.M.B., Garrido, V., Bisso, M., Antunes, D.M., Nobrega, A.F. & Teixeira, G. (2009) Food allergy/hypersensitivity: antigenicity or timing? Immunobiology 214, 269–278.Google Scholar
Pelayo, V., García-Hernández, P., Puente, P., Rodero, M. & Cuéllar, C. (2009) Seroprevalence of anti-Gymnorhynchus gigas (Trypanorhyncha, Gymnorhynchidae) antibodies in a Spanish population. Journal of Parasitology 95, 778–780.CrossRefGoogle Scholar
Puente, P., Anadón, A.M., Rodero, M., Romarís, F., Ubeira, F.M. & Cuéllar, C. (2008) Anisakis simplex: the high prevalence in Madrid (Spain) and its relation with fish consumption. Experimental Parasitology 118, 271–274.Google Scholar
Rodero, M. & Cuéllar, C. (1999) Humoral immune responses induced by Gymnorhynchus gigas extracts in BALB/c mice. Journal of Helminthology 73, 239–243.CrossRefGoogle ScholarPubMed
Van der Ventel, M.L., Nieuwenhuizen, N.E., Kirstein, F., Hikuam, C., Jeebhay, M.F., Swoboda, I., Brombacher, F. & Lopata, A.L. (2011) Differential responses to natural and recombinant allergens in a murine model of fish allergy. Molecular Immunology 48, 637–646.Google Scholar
Vázquez-López, C., De Armas-Serra, C., Bernardina, W. & Rodríguez-Caabeiro, F. (2001) Oral inoculation with Gymnorhynchus gigas induces anti-parasite anaphylatic antibody production in both mice and rats and adverse reactions in challenge mice. International Journal of Food Microbiology 64, 307–315.Google Scholar
Vázquez-López, C., De Armas-Serra, C., Bernardina, W. & Rodríguez-Caabeiro, F. (2002) A 24-kDa collagenase from Gymnorhynchus gigas elicits rat ileum hyperreactivity and is a target of humoral responses in mice previously given a single oral dose of parasite extract. Digestive Diseases Sciences 47, 935–942.Google Scholar