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Immunoprecipitins in human schistosomiasis detected with adult worm antigens released by 3M KC1

Published online by Cambridge University Press:  05 June 2009

Mauro Scapin  and
Miriam Tendler
Mauro Scapin
Affiliation:
Department of Biophysics, Gama Filho University, Rio de Janeiro
Miriam Tendler
Affiliation:
Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil
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Extract

Several methods are being applied for solubilizing adult Schistosoma mansoni antigens which may allow their immunochemical characterization and purification. Studies on antigens and immunoprecipitins in schistosome infections have been carried out with homogenized extracts of adult worms and/or larval forms of Schistosoma mansoni in saline (Biguet et al., 1962; Kagan and Norman, 1963; Silva and Ferri, 1965), or water (Kent, 1963). Kusel (1972) studied surface proteins in S. mansoni membrane, treating the worms with 0·5% saponin in 3% calcium chloride. Murrel et al. (1974) compared by immunodiffusion a crude culture antigen with extracts obtained by freeze-thawing of adult worms and by treatment in 3M KCl.

Type
Research Note
Information
Journal of Helminthology , Volume 51 , Issue 1 , March 1977 , pp. 71 - 72
Copyright
Copyright © Cambridge University Press 1977

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References

REFERENCES

Biguet, J., Capron, A. and Tran Van Ky, P. (1962) Les antigènes de Schistosoma mansoni. 1—Étude électrophorétique et immunoélectrophorétique. Caracterization des antigènes specifiques. Annales de l'Institut Pasteur, 103, 763–777.Google Scholar
Deelder, A. M., Kornelis, D., Rier, W. B. and Ten-Jet-Foei, C. L. (1975) Immunodiagnosis of human schistosomiasis using different immunoprecipitation techniques. Zeitschrift für Parasitenkunde, 47, 111–118.CrossRefGoogle ScholarPubMed
Hillyer, G. V. and Ritchie, L. S. (1967) Immunoprecipitins in Schistosoma mansoni infections. II. Cercopithecus sabaens and Macaca mulatta monkeys. Experimental Parasitology, 20, 326–333.Google Scholar
Kagan, I. G. and Norman, L. (1963) Analysis of helminth antigens (Ecchinococcus granulosus and Schistosoma mansoni) by agar gel methods. Annals of New York Academy of Science, 113, 130–153.CrossRefGoogle Scholar
Kent, N. H. (1963) Comparative immunochemistry of larval and adult forms of Schistosoma mansoni. Annals of New York Academy of Science, 113, 100–113.CrossRefGoogle Scholar
Kusel, J. R. (1972) Protein composition and protein synthesis in the surface membranes of Schistosoma mansoni. Parasitology, 65, 55–69.Google Scholar
Murrell, K. D., Vannier, W. E. and Alimed, A. (1974) Schistosoma mansoni: antigenic heterogeneity of excretions and secretions. Experimental Parasitology, 36, 316–330.CrossRefGoogle ScholarPubMed
Scapin, M. and Tendler, M. (1975) Immunoelectroosmophoresis as a rapid method for detecting soluble antigenic fraction of Schistosoma mansoni. Journal of Parasitology, 61, 561–562.Google Scholar
Silva, L. C. and Ferri, R. G. (1965) Immunodiffusion studies in human schistosomiasis mansoni. I—Hepatointestinal and hepatosplenic forms. Revista do Instituto de Medicina Tropical de São Paulo, 7, 1–6.Google ScholarPubMed