Hostname: page-component-78c5997874-mlc7c Total loading time: 0 Render date: 2024-11-14T04:31:48.211Z Has data issue: false hasContentIssue false
  • English
  • Français

Insulin-like effects of fatty acid synthesis in liver of hamsters infected with plerocercoids of the tapeworm, Spirometra mansonoides

Published online by Cambridge University Press:  18 November 2009

C. Kirk Phares  and
R. M. Carroll
C. Kirk Phares
Affiliation:
Department of Biochemistry, University of Nebraska, Omaha, Nebraska 68105, U.S.A.;
R. M. Carroll
Affiliation:
Department of Comparative Medicine, Bowman Gray Medical School, Winston-Salem, North Carolina 27103, U.S.A.
Get access Rights & Permissions [Opens in a new window]

Abstract

Elevated serum lipids are associated with infections of laboratory rodents with plerocercoids of Spirometra mansonoides. The effect of infection with these larval tapeworms on triglyceride degradation and hepatic de novo fatty acid synthesis was investigated in Syrian hamsters. Serum lipoprotein electrophoresis revealed a consistent elevation in very low density lipoproteins in the infected animals. Lipoprotein lipase activity was enhanced in the infected animals. After seven days of plerocercoid infection the activity of acetyl-CoA carboxylase (E.C. 6.4.1.2) was significantly elevated after 6,12 and 18 hours of fasting. Fatty acid synthetase was significantly increased after 0,6,12,18 and 24 hours of fasting. Therefore, a chronic insulin-like activity on lipid metabolism of hamsters is associated with plerocercoid infection.

Type
Research Article
Information
Journal of Helminthology , Volume 58 , Issue 1 , March 1984 , pp. 25 - 30
Copyright
Copyright © Cambridge University Press 1984

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bagdade, J.D., Porte, D. Jr., & Bierman, E.L. (1968) Acute insulin withdrawal and the regulation of plasma triglyceride removal in diabetic subjects. Diabetes, 17, 127–132.CrossRefGoogle ScholarPubMed
Beynen, A.C., Vaartjes, W.J. & Geelen, M.J.H. (1979) Opposite effects of insulin and glucagon in acute hormonal control of hepatic lipogensis. Diabetes, 28, 828–835.CrossRefGoogle Scholar
Birnbaum, R.S. & Goodman, H.M. (1979) Comparison of several insulin-like effects of growth hormone. Hormone and Metabolism Research, 11, 185–186.CrossRefGoogle ScholarPubMed
Garland, J.T., Ruegamer, W.R. & Daughaday, W.H. (1971) Induction of sulfation factor activity by infection of hypophysectomized rats with Spirometra mansonoides. Endocrinology, 88, 924–927.CrossRefGoogle ScholarPubMed
Gibson, D.M. & Hubbard, D.D. (1960) Incorporation of malonyl-CoA into fatty acids by liver in starvation and alloxan-diabetes. Biochemical and Biophysical Research Communications, 3, 531–535.CrossRefGoogle ScholarPubMed
Gujral, S. & Rajgor, N. (1981) Serum lipid values in golden hamster infected with Ancylostoma ceylanicum. Journal of Parasitology, 67, 758–759.CrossRefGoogle ScholarPubMed
Harlow, D.R., Mertz, W. & Mueller, J.F. (1967) Insulin-like activity from the sparganum of Spirometra mansonoides. Journal of Parasitology, 53, 449–454.CrossRefGoogle ScholarPubMed
Hsu, R.Y., Butterworth, P.H.W. & Porter, J.W. (1969) Pigeon liver fatty acid synthase. In: Methods in Enzymology (Lowenstein, J.M., Editor) Academic Press, New York, pp. 33–38.Google Scholar
Jacobs, R.A., Sly, W.S. & Majerus, P.W. (1973) The regulation of fatty acid biosynthesis in human skin fibroblasts. Journal of Biological Chemistry, 248, 1268–1276.CrossRefGoogle ScholarPubMed
Korn, E.D. (1959) The assay of lipoprotein lipase in vivo and in vitro. In: Methods of Biochemical Analysis (Glick, D., Editor), Interscience Publisher Inc., New York, pp. 145–192.CrossRefGoogle Scholar
Lowry, O.H., Rosebrough, N.J., Farr, A.L. & Randall, R.J. (1951) Protein measurement with the folin phenol reagent. Journal of Biological Chemistry, 193, 265–275.CrossRefGoogle ScholarPubMed
Merimee, T.J. (1979) Endocrinology 1, Grune and Stratton, New York, pp. 123–132.Google Scholar
Merimee, T.J. & Rabin, D. (1973) A survey of growth hormone secretion and action. Metabolism, 22, 1235–1251.CrossRefGoogle ScholarPubMed
Meyer, F., Kimura, S. & Mueller, J.F. (1965) Stimulation of lipogenesis in hamsters by Spirometra mansonoides. Journal of Parasitology, 51 (Suppl.), 143.Google Scholar
Mueller, J.F. (1966) The laboratory propagation of Spirometra mansonoides (Mueller, 1935) as an experimental tool VII. Improved techniques and additional notes on the biology of the cestode. Journal of Parasitology, 52, 437–443.CrossRefGoogle ScholarPubMed
Mueller, J.F. (1974) The biology of Spirometra. Journal of Parasitology, 60, 3–14.CrossRefGoogle ScholarPubMed
Numa, S. & Yamashita, S. (1974) Regulation of lipogenesis in animal tissues. Current Topics in Cellular Regulation, 8, 197–246.CrossRefGoogle ScholarPubMed
Phares, C.K. & Carroll, R.M. (1977) A lipogenic effect in intact male hamsters infected with plerocercoids of the tapeworm, Spirometra mansonoides. Journal of Parasitology, 63, 690–693.CrossRefGoogle ScholarPubMed
Phares, C.K. & Carroll, R.M. (1978) Comparison of the effects of the growth factor produced by Spirometra mansonoides and growth hormone in diabetic-hypophysectomized rats: Lipid Composition. Journal of Parasitology, 64, 401–405.CrossRefGoogle ScholarPubMed
Rouzer, C.A. & Cerami, A. (1980) Hypertriglyceridemia associated with Trypanosoma brucei brucei infection in rabbits: role of defection triglyceride removal. Molecular and Biochemical Parasitology, 2, 31–38.CrossRefGoogle Scholar
Sadun, E.H., Williams, J.S., Maroney, F.C. & Mueller, J.F. (1965) Biochemical changes in mice infected with spargana of cestode, Spirometra mansonoides. Journal of Parasitology, 51, 532–536.CrossRefGoogle ScholarPubMed
Soloni, F.G. & Sardina, L.C. (1973) Colorimetric microdetermination of free fatty acids. Clinical Chemistry, 19, 419–424.CrossRefGoogle ScholarPubMed
Steelman, S.L., Glitzer, M.S., Ostlind, D.A. & Mueller, J.F. (1971) Biological properties of the growth hormone-like factor from the pleroccrcoid of Spirometra mansonoides. Recent Progress in Hormone Research, 27, 97–120.Google Scholar
Vanwyk, J.J., Underwood, L.E., Hintz, R.L., Clemmons, D., Voina, S.J. & Weaver, R.P. (1974) The somatomedins: a family of insulin-like hormones under growth hormone control. Recent Progress in Hormone Research, 30, 259–294.Google Scholar
Witters, L.A., Moriarity, D. & Martin, D. (1979) Regulation of hepatic acctyl cocnzyme a carboxylase by insulin and glucagon. Journal of Biological Chemistry, 254, 6644–6649.CrossRefGoogle Scholar