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Intra-species variability in ITS-1 sequences of Haemonchus contortus isolated from goats in West Bengal, India

Published online by Cambridge University Press:  31 August 2010

S. Bandyopadhyay ,
A.K. Bera ,
S. Sikdar ,
S. De ,
S. Das ,
T. Rana ,
D. Pan ,
S. Bandyopadhyay  and
D. Bhattacharya
S. Bandyopadhyay*
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata37, West Bengal, India
A.K. Bera
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata37, West Bengal, India
S. Sikdar
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata37, West Bengal, India
S. De
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata37, West Bengal, India
S. Das
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata37, West Bengal, India
T. Rana
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata37, West Bengal, India
D. Pan
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata37, West Bengal, India
S. Bandyopadhyay*
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata37, West Bengal, India
D. Bhattacharya
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata37, West Bengal, India
*
*E-mail: subuicar@rediffmail.com
*E-mail: subuicar@rediffmail.com
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Abstract

This study evaluated the existence of different genotypes of Haemonchus contortus prevailing among goats in West Bengal, India. These parasites were isolated from the abomasum of goat intestine and the molecular characterization was performed by comparing variation of nucleotide sequences of the internal transcribed spacer 1 (ITS-1) gene region. Single-strand conformation polymorphism (SSCP) analysis of ITS-1 amplified product showed the presence of three distinct conformations both in male and female parasites. The sequence analysis of conformations showed two single nucleotide polymorphisms (SNP) in male parasites at nucleotide positions 106 and 107 and one SNP was detected in female parasites at nucleotide position 157. These nucleotide variations in different isolates did not alter the interior loop structure of the predicted secondary RNA, therefore we believe these variations may not be responsible for any evolutionary changes among conformations.

Type
Regular research papers
Information
Journal of Helminthology , Volume 85 , Issue 2 , June 2011 , pp. 204 - 209
Copyright
Copyright © Cambridge University Press 2010

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