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Leukocyte accumulation in sparganosis: further characterization of an eosinophil chemotactic factor of the plerocercoid of Spirometra erinacei

Published online by Cambridge University Press:  05 June 2009

Y. Horii ,
M. Owhashi ,
A. Ishii  and
K. Fujita
Y. Horii
Affiliation:
Department of Medical Zoology, Nagasaki University School of Medicine, Sakamoto-machi, Nagasaki 852, Japan
M. Owhashi
Affiliation:
Department of Parasitology, Miyazaki Medical College, Kiyotake, Miyazaki 889–16, Japan
A. Ishii
Affiliation:
Department of Parasitology, Okayama University Medical School, Shikata-cho, Okayama 700, Japan
K. Fujita
Affiliation:
Department of Medical Zoology, Nagasaki University School of Medicine, Sakamoto-machi, Nagasaki 852, Japan
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Abstract

An eosinophil chemotactic factor (ECF) was partially purified from plerocercoids of Spirometra erinacei by a combination of anion-exchange chromatography on DE52 and gel filtration on Sephacryl S–200. The molecular weight of ECF was estimated to be 25 000–45 000 by high-pressure liquid chromatography. The ECF was bound with concanavalin A-Sepharose. The ECF was sensitive to periodate oxidation and to heating (56°C, 30 min). On isoelectric focusing, eosinophil chemotactic activity was clearly revealed at pI 4·1. These results suggest that ECF of S. erinacei plerocercoid is an acidic glycoprotein. An intradermal injection of ECF induced eosinophil attractions in the normal guinea pig skin.

Keywords

Spirometra erinaceieosinophil chemotactic factor
Type
Research Article
Information
Journal of Helminthology , Volume 63 , Issue 1 , March 1989 , pp. 6 - 12
Copyright
Copyright © Cambridge University Press 1989

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