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Mitochondrial DNA polymorphism of a triploid form of Fasciola in Japan

Published online by Cambridge University Press:  07 February 2017

T. Itagaki ,
M. Honnami ,
D. Ito ,
K. Ito ,
K. Tsutsumi ,
K. Terasaki ,
T. Shibahara  and
Y. Noda
T. Itagaki*
Affiliation:
Department of Parasitology, Iwate University, Morioka 020-8550, Japan
M. Honnami
Affiliation:
Department of Parasitology, Iwate University, Morioka 020-8550, Japan
D. Ito
Affiliation:
Department of Parasitology, Iwate University, Morioka 020-8550, Japan
K. Ito
Affiliation:
Institute for Cell Biology and Genetics, Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan
K. Tsutsumi
Affiliation:
Institute for Cell Biology and Genetics, Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan
K. Terasaki
Affiliation:
St Mary's Junior College, Kurume, Fukuoka 830-8558, Japan
T. Shibahara
Affiliation:
Laboratory Animal Research Center, Faculty of Medicine, Tottori University, Yonago 683, Japan
Y. Noda
Affiliation:
Institute of Animal Experiment, Kurume University School of Medicine, Kurume, Fukuoka 830-0011, Japan
*
*Fax: 81 19 621 6219 E-mail: itagaki@iwate-u.ac.jp
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Abstract

Mitochondrial DNA polymorphism was characterized in a triploid form of Fasciola found in Japan in comparison with F. hepatica, F. gigantica and Korean Fasciola worm. Seventy worms of Fasciola from Japan, three of F. hepatica from Uruguay and Australia, two of F. gigantica from Thailand and one of Fasciola from Korea were used in the study. Mitochondrial DNA polymorphism was detected by restriction fragment length polymorphism (RFLP) using eight restriction enzymes, BamH I, Bgl II, Dra I, EcoR I, EcoR V, Hind III, Mfl I and Sca I. Three different types (types 1, 2 and 3) were detected from 76 Fasciola worms used in the study. Eight of 70 Japanese worms were categorized in type 2 (F. gigantica type), and the remaining 62 were in type 3 (F. hepatica type).

Type
Research Article
Information
Journal of Helminthology , Volume 75 , Issue 2 , June 2001 , pp. 193 - 196
Copyright
Copyright © Cambridge University Press 2001

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