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Morphological and molecular analyses of the spiruroid nematode, Falcaustra araxiana Massino, 1924 (= Spironoura araxiana) from the European pond turtle (Emys orbicularis)

Published online by Cambridge University Press:  01 April 2016

M. Rajabloo ,
H. Sharifiyazdi ,
F. Namazi ,
H. Shayegh ,
E. Rakhshandehroo  and
G. Farjanikish
M. Rajabloo
Affiliation:
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, PO Box 1731, Shiraz 71345, Iran
H. Sharifiyazdi*
Affiliation:
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, PO Box 1731, Shiraz 71345, Iran
F. Namazi
Affiliation:
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, PO Box 1731, Shiraz 71345, Iran
H. Shayegh
Affiliation:
School of Veterinary Medicine, Shiraz University, Shiraz, Iran
E. Rakhshandehroo
Affiliation:
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, PO Box 1731, Shiraz 71345, Iran
G. Farjanikish
Affiliation:
Department of Pathobiology, School of Veterinary Medicine, Lorestan University, Khorram Abad, Iran
*
*E-mail: Hassansharifi@gmail.com
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Abstract

There is little information on the phylogenetic position and life cycle of family Kathlaniidae. Falcaustra araxiana is a member of this family which infects the large intestine of the European pond turtle (Emys orbicularis). In the present study, morphological data and molecular analyses based on the 18S rDNA were performed on different types of F. araxiana originating from the large intestine and gastric nodules in the turtle. Morphological data revealed both larvae and adult stages in the gastric nodules. In addition, all nematodes recovered in the large intestine were adult worms. GenBank accession numbers KM200715 and KM200716 were provided for adult F. araxiana located in the intestine and stomach, respectively, of E. orbicularis. The results of sequencing proved that these two types are completely similar. Accordingly, it can be hypothesized that nodule formation is a part of the life cycle of the parasite or a survival strategy. Furthermore, F. araxiana develops to the adult stage in the gastric mucosa prior to migrating to the large intestine. Phylogenetic analysis revealed that F. araxiana unexpectedly branched away from other members of the superfamily Seuratoidea (Truttaedacnitis truttae, Cucullanus robustus and C. baylisi) and showed a closer relationship with Paraquimperia africana, a member of the Ascaridoidea. It seems that phylogenetic reconstruction for the present parasite needs more detailed morphology, life cycle and molecular studies.

Type
Research Papers
Information
Journal of Helminthology , Volume 91 , Issue 3 , May 2017 , pp. 356 - 359
Copyright
Copyright © Cambridge University Press 2016 

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