Hostname: page-component-78c5997874-lj6df Total loading time: 0 Render date: 2024-11-10T10:49:51.311Z Has data issue: false hasContentIssue false
  • English
  • Français

Morphological and molecular data of two species of the rare genera Thada Thorne, 1941 and Tenunemellus Siddiqi, 1986 (Nematoda: Tylenchidae) from Iran

Published online by Cambridge University Press:  04 May 2020

M. Hosseinvand ,
A. Eskandari Open the ORCID record for A. Eskandari [Opens in a new window] ,
R. Ghaderi  and
A. Karegar
M. Hosseinvand
Affiliation:
Department of Plant Protection, College of Agriculture, University of Zanjan, 45371-38791Zanjan, Iran
A. Eskandari*
Affiliation:
Department of Plant Protection, College of Agriculture, University of Zanjan, 45371-38791Zanjan, Iran
R. Ghaderi
Affiliation:
Department of Plant Protection, School of Agriculture, University of Shiraz, 71441-65186Shiraz, Iran
A. Karegar
Affiliation:
Department of Plant Protection, School of Agriculture, University of Shiraz, 71441-65186Shiraz, Iran
*
Author for correspondence: A. Eskandari, E-mail: eskandari.a@znu.ac.ir
Get access Rights & Permissions [Opens in a new window]

Abstract

Thada populus n. sp. was found in the rhizosphere of Populus euphratica in the city of Dezful, south-western Iran. The new species is characterized by its 365–453 μm body length, cuticle with transverse annuli 0.9–1.2 μm wide, lateral fields with four lines, lip region low with one or two annuli, 7.0–8.5 μm wide at base, longitudinal or slightly sigmoid amphidial slit, delicate stylet 8.4–9.8 μm long with posteriorly directed knobs, dorsal pharyngeal gland opening at 1.0–1.5 μm posterior to the stylet knobs, non-muscular and valve-less median bulb, pyriform and offset basal bulb, almost oval spermatheca, posterior position of vulva (V = 75–79%) without lateral membrane, short post-vulval uterine sac and conical tail with finely to broadly rounded terminus. Morphological differences of the new species with those of the type species, Thada striata, are discussed. Molecular phylogenetic studies of the new species using partial sequences of small subunit ribosomal DNA revealed that the new species formed a clade with Malenchus spp., Filenchus facultativus, F. fungivorus and Filenchus sp. in Bayesian inference. Morphological and molecular phylogenetic studies were also performed on Tenunemellus indicus, the second recovered species, the latter analysis using partial sequences of large subunit ribosomal DNA. In the inferred Bayesian tree, T. indicus formed a clade with Labrys fuzhouensis, Lelenchus leptosoma from the Netherlands, Malenchus spp. and Filenchus discrepans.

Keywords

18S rDNA28S rDNABoleodorinaecity of DezfulEcphyadophorinaeidentificationKhuzestan provinceredescriptionTenunemellus indicusThadinaeThada populus n. sp.Tylenchinae
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e149
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Andrássy, I (1954) Revision der Gattung Tylenchus Bastian, 1865 (Tylenchidae, Nematoda). Acta Zoologica Academiae Scientiarum Hungaricae 1, 5–42.Google Scholar
Andrássy, I (1958) Erd- und Süsswassernematoden aus Bulgarien. Acta Zoologica Academiae Scientiarum Hungaricae 4, 1–88.Google Scholar
Andrássy, I (1959) Freilebende Nematoden aus Rumänien. Annales Universitatis Scientarum Budapestensis de Rolando Eötvös nominate, Sectio Biologica 2, 3–27.Google Scholar
Andrássy, I (1972) A Magyarországról eddig kimutatott szabadon élő fonálférgek (Nematoda) jegyzéke. Állattani Közlemények 59, 161–171.Google Scholar
Andrássy, I (1981) The genera and species of the family Tylenchidae Örley, 1880 (Nematoda). The genus Malenchus Andrássy, 1968. Acta Zoologica Academiae Scientarum Hungaricae 27, 1–47.Google Scholar
Bastian, HC (1865) Monograph on the Anguillulidae, or free nematoids, marine, land and freshwater; with descriptions of 100 new species. Transactions of the Linnean Society of London 25, 73–184.CrossRefGoogle Scholar
Bert, W, Okada, H, Tavernier, I, Borgonie, G and Houthoofd, W (2010) Morphological, morphometrical and molecular characterisation of Filenchus fungivorus n. sp., a fungivorous nematode from Japan in a most likely polyphyletic genus (Nematoda: Tylenchina). Nematology 12, 235–246.CrossRefGoogle Scholar
Castresana, J (2000) Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Molecular Biology and Evolution 17, 540–552.CrossRefGoogle ScholarPubMed
Corbett, DCM (1964) Central African nematodes. I. Ecphyadophora quadralata n. sp. and two species of Ecphyadophoroides n. gen. (Nematoda: Neotylenchidae). Nematologica 10, 121–130.CrossRefGoogle Scholar
Darriba, D, Taboada, GL, Doallo, R and Posada, D (2012) jModel Test 2: more models, new heuristics and parallel computing. Nature Methods 9, 772.CrossRefGoogle Scholar
Decraemer, W and Hunt, DJ (2013) Structure and classification. pp. 3–39in Perry, RN and Moens, M (Eds) Plant nematology. 2nd edn. Wallingford, Oxfordshire, UK, and Boston, MA, USA, CABI Publishing.CrossRefGoogle Scholar
De Grisse, A (1969) Redescription ou modification de quelques techniques utilisees dans l’étude des nématodes phytoparasitaires. Mededelingen Rijksfaculteit Landbouwwetenschappen Gent 34, 351–369.Google Scholar
De Man, JG (1880) Die einheimischen, frei in der reinen Erde und im süßen wasser lebenden Nematoden. Vorläufige Bericht und descriptiv-systematischer Theil. Tijdschrift der Nederlandsche Dierkundige Vereeniging 5, 1–104.Google Scholar
De Man, JG (1921) Nouvelles recherches sur les nematodes terricoles de la Hollande. Capita Zoologica 1, 3–62.Google Scholar
Eisvand, P, Farrokhi Nejad, R and Azimi, S (2019) Description of Basiria khouzestanensis n. sp. (Nematoda: Tylenchidae) from Iran and its phylogenetic relationships with other species in the family. Zootaxa 4563(3), 482–490.CrossRefGoogle ScholarPubMed
Filipjev, IN (1936) On the classification of the Tylenchinae. Proceedings of the Helminthological Society of Washington 3, 80–82.Google Scholar
Fortuner, R and Maggenti, R (1987) A reappraisal of Tylenchina (Nemata). 4. The family Anguinidae Nicoll, 1935. Revue de Nématologie 10(2), 163–176.Google Scholar
Geraert, E (1974) The morphology and the systematic position of the genus Thada (Nematoda: Tylenchida). Mededelingen faculteit Landbouwwetenschappen Gent 39, 701–712.Google Scholar
Geraert, E (2008). The Tylenchidae of the world, identification of the family Tylenchidae (Nematoda: Tylenchida). 540 pp. Ghent, Belgium, Academia Press.Google Scholar
Geraert, E and Raski, DJ (1987) A reappraisal of Tylenchina (Nemata). 3. The family Tylenchidae Örley, 1880. Revue de Nématologie 10, 143–161.Google Scholar
Goodey, JB (1962) Tylenchus (Cephalenchus) megacephalus n. sbg., n. sp. Nematologica 7, 331–333.CrossRefGoogle Scholar
Holterman, M, van der Wurff, A, van den Elsen, S, van Megen, H, Bongers, T, Holovachov, O, Bakker, J and Helder, J (2006) Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown clades. Molecular Biology and Evolution 23, 1792–1800.CrossRefGoogle ScholarPubMed
Hosseinvand, M, Eskandari, A and Ghaderi, R (2019) Morphological and molecular characterization of Coslenchus paramaritus n. sp. and C. cancellatus (Cobb, 1925) Siddiqi, 1978 (Nematoda: Tylenchidae) from Iran. Journal of Nematology 51, e2019-59.CrossRefGoogle Scholar
Huelsenbeck, JP and Ronquist, F (2001) MrBayes: Bayesian inference of phylogeny. Bioinformatics 17, 754–755.CrossRefGoogle Scholar
Husain, SI and Khan, AM (1967) A new subfamily, a new subgenus and eight new species of Nematodes from India belonging to superfamily Tylenchoidea. Proceedings of the Helminthological Society of Washington 34, 175–186.Google Scholar
Husain, SI and Khan, AM (1968) Ecphyadophoroides graminis n. sp. and two new species of Ecphyadophora (Nematoda: Ecphyadophorinae) from North India. Nematologica 14, 377–384.Google Scholar
Jalalinasab, P, Adeldoost, Y, Abolafia, J and Heydari, R (2019) Description of Malenchus gilanensis n. sp. from Iran. Zootaxa 4638(4), 562–570.CrossRefGoogle ScholarPubMed
Karegar, A (2018) Nematodes of the family Tylenchidae Örley, 1880 sensu lato. pp. 459–586in Ghaderi, R, Kashi, L and Karegar, A (Eds) Plant-parasitic nematodes in Iran. Shiraz, Marjaeelm Press & Iranian Society of Nematology (in Persian).Google Scholar
Khan, E (1964a) Boleodorus mirus n. sp. (Tylenchidae: Boleodorinae n. subfam.) from Kufri, Simla (H.P.), India, with a key to the species of the genus Boleodorus Thome, 1941. Zoologischer Anzeiger 173, 336–341.Google Scholar
Khan, SH (1964b). Sakia typica n. g., n. sp. (Nematoda: Neotylenchidae) from North India. Proceedings 51st & 52nd Indian Science Congress III, p. 467.Google Scholar
Khan, SH (1973) On the proposal for Neothada n. gen. (Nematoda: Tylenchinae). Proceedings of the National Academy of Sciences, India, Biological Sciences 43, 17–18.Google Scholar
Kumar, S, Stecher, G and Tamura, K (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33, 1870–1874.CrossRefGoogle ScholarPubMed
Nicoll, W (1935) Rhabditida. Anguinidae. VI. Vermes. Zoological Record 72, 105.Google Scholar
Örley, L (1880) Monograph of the anguillulids. Természetrajzi Füzetek 4, 16–150.Google Scholar
Panahandeh, Y, Abolafia, J, Pourjam, E, Jahanshahi Afshar, F, Giblin-Davis, RM and Pedram, M (2018) Morphological and Molecular Characterization of Labrys filiformis n. sp. (Rhabditida: Tylenchidae) from Iran. Journal of Nematology 50, 343–354.CrossRefGoogle ScholarPubMed
Panahandeh, Y, Pourjam, E, Álvarez-Ortega, S, Jahanshahi Afshar, F and Pedram, M (2019a) New observations on Miculenchus Andrássy, 1959 (Nematoda: Tylenchidae) with descriptions of two new and one known species from Iran. Nematology 21, 937–956.CrossRefGoogle Scholar
Panahandeh, Y, Pourjam, E, Abolafia, J, Roshan-bakhsh, A, Mojerlou, SH, Jahanshahi Afshar, F and Pedram, M (2019b) Labrys khuzestanensis n. sp. (Nematoda, Tylenchidae), a new member of the genus with large labial plate. Zootaxa 4671(2), 267–276.CrossRefGoogle Scholar
Panahandeh, Y, Atighi, MR, De Ley, IT, Pourjam, E, Mundo-Ocampo, M, Abolafia, J, Koolivand, D, Jahanshahi Afshar, F and Pedram, M (2019c) An integrative study of Sakia sisanganensis n. sp. (Rhabditida: Tylenchidae) from Sisangan forest, Iran, and new morphological observations for the genus. Forest Pathology 49, e12536.CrossRefGoogle Scholar
Qiao, K, Bai, M, He, Y, Chen, J, Xiao, S, Cheng, X, Liu, G, Braun-Miyara, S and Qing, X (2019) Description of Labrys fuzhouensis sp. n. and first record of Coslenchus rafiqi (Nematoda: Tylenchidae) from China. Nematology 21, 693–708.CrossRefGoogle Scholar
Qing, X and Bert, W (2018) 3D printing in zoological systematics: Integrative taxonomy of Labrys chinensis gen. nov., sp. nov. (Nematoda: Tylenchomorpha). Journal of Zoological Systematics and Evolutionary Research 56(1), 35–47.CrossRefGoogle Scholar
Rambaut, A (2016) Figtree, a graphical viewer of phylogenetic trees. Available at: http://tree.bio.ed.ac.uk/software/figtree.Google Scholar
Raski, DJ and Geraert, E (1987) Review of the genus Filenchus Andrássy, 1954 and descriptions of six new species (Nemata: Tylenchidae). Nematologica 32, 265–311 (1986).Google Scholar
Raski, DJ, Koshy, PK and Sosamma, VK (1982) A revision of the subfamily Ecphyadophorinae Skarbilovich, 1959 (Tylenchidae: Nematoda). Revue de Nématologie 5, 119–138.Google Scholar
Ronquist, F, Teslenko, M, van der Mark, P, et al. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61, 539–542.CrossRefGoogle ScholarPubMed
Siddiqi, MR (1959) Basiria graminophila n. g., n. sp., (Nematoda: Tylenchinae) found associated with grass roots in Aligarh, India. Nematologica 4, 217–222.CrossRefGoogle Scholar
Siddiqi, MR (1978) The unusual position of the phasmids in Coslenchus costatus (de Man, 1921) gen. n., comb. n., and other Tylenchidae (Nematoda: Tylenchida). Nematologica 24, 449–455.CrossRefGoogle Scholar
Siddiqi, MR (1979) Seven new species in a new nematode subfamily Duosulciinae (Tylenchidae), with proposals for Duosulcius gen. n., Zanenchus gen. n. and Neomalenchus gen. n. Nematologica 25, 215–236.CrossRefGoogle Scholar
Siddiqi, MR (1986). Tylenchida: parasites of plants and insects. 645 pp. Farnham Royal, London, Commonwealth Agricultural Bureaux.Google Scholar
Siddiqi, MR (2000). Tylenchida: parasites of plants and insects. 833 pp. Wallingford, UK, CABI Publishing.CrossRefGoogle Scholar
Soleymanzadeh, M, Pedram, M, Pourjam, E and Álvarez-Ortega, S (2016) Description of Lelenchus brevislitus n. sp. (Nematoda: Tylenchidae), an example of a cryptic species from Iran and its phylogenetic relationships with other species in the family. Nematology 18, 987–998.CrossRefGoogle Scholar
Subbotin, SA, Sturhan, D, Chizhov, VN, Vovlas, N and Baldwin, JG (2006) Phylogenetic analysis of Tylenchida Thorne, 1949 as inferred from D2 and D3 expansion fragments of the 28S rRNA gene sequences. Nematology 8, 455–474.CrossRefGoogle Scholar
Tanha Maafi, Z, Subbotin, SA and Moens, M (2003) Molecular identification of cyst-forming nematodes (Heteroderidae) from Iran and a phylogeny based on ITS-rDNA sequences. Nematology 5, 99–111.CrossRefGoogle Scholar
Thorne, G (1941) Some nematodes of the family Tylenchidae, which do not possess a valvular median oesophageal bulb. Great Basin Naturalist 2, 37–85.Google Scholar
Thorne, G and Malek, RB (1968). Nematodes of the Northern Great Plains. Part I. Tylenchida (Nemata: Secernentea). 111 pp. Brookings, South Dakota, Agricultural Experiment Station, South Dakota State University.Google Scholar
Verma, RS (1972) Three new species of nematodes in the subfamily Ecphyadophorinae associated with cereal crops in Uttar Pradesh (India). Zoologischer Anzeiger 189, 89–94.Google Scholar
Whitehead, AG and Hemming, JR (1965) A comparison of some quantitative methods for extracting small vermiform nematodes from soil. Annals of Applied Biology 55, 25–38.CrossRefGoogle Scholar