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Pathology associated with three new Clinostomum metacercariae from Argentina with morphological and DNA barcode identification

Published online by Cambridge University Press:  04 May 2020

M.M. Montes Open the ORCID record for M.M. Montes [Opens in a new window] ,
S.E. Plaul Open the ORCID record for S.E. Plaul [Opens in a new window] ,
Y. Croci Open the ORCID record for Y. Croci [Opens in a new window] ,
M. Waldbillig ,
W. Ferrari Open the ORCID record for W. Ferrari [Opens in a new window] ,
E. Topa Open the ORCID record for E. Topa [Opens in a new window]  and
S.R. Martorelli Open the ORCID record for S.R. Martorelli [Opens in a new window]
M.M. Montes
Affiliation:
Centro de Estudios Parasitológicos y Vectores (CEPAVE), Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de La Plata (CCT, CONICET-UNLP), calle 120 s/n e/60 y 64, 1900, La Plata, Buenos Aires, Argentina
S.E. Plaul*
Affiliation:
Laboratorio de Histología y Embriología Descriptiva, Experimental y Comparada, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Av. 60 y 118 s/n., La Plata, Buenos Aires, Argentina
Y. Croci
Affiliation:
Centro de Estudios Parasitológicos y Vectores (CEPAVE), Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de La Plata (CCT, CONICET-UNLP), calle 120 s/n e/60 y 64, 1900, La Plata, Buenos Aires, Argentina
M. Waldbillig
Affiliation:
Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Av. 60 y 120 s/n, La Plata, Buenos Aires, Argentina
W. Ferrari
Affiliation:
Centro de Estudios Parasitológicos y Vectores (CEPAVE), Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de La Plata (CCT, CONICET-UNLP), calle 120 s/n e/60 y 64, 1900, La Plata, Buenos Aires, Argentina
E. Topa
Affiliation:
Centro de Estudios Parasitológicos y Vectores (CEPAVE), Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de La Plata (CCT, CONICET-UNLP), calle 120 s/n e/60 y 64, 1900, La Plata, Buenos Aires, Argentina
S.R. Martorelli
Affiliation:
Centro de Estudios Parasitológicos y Vectores (CEPAVE), Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de La Plata (CCT, CONICET-UNLP), calle 120 s/n e/60 y 64, 1900, La Plata, Buenos Aires, Argentina
*
Author for correspondence: S.E. Plaul, E-mail: splaul@fcnym.unlp.edu.ar
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Abstract

In the Laboratory of Parasites of Fishes, Crustaceans and Mollusks (CEPAVE), we undertook a parasitological study on three species of fish from the Espinal and Esteros del Iberá ecoregions of Argentina. Clinostomid metacercariae were found parasitizing Characidium rachovii, Crenicichla vittata and Gymnogeophagus balzanii. In this study, we analysed the damage that these parasites inflict on their hosts through the evaluation of histological sections. In addition, Clinostomum metacercariae were identified using morphological characters and DNA barcoding. In the pathological analysis, we observed that muscle tissue was the most affected. The inflammatory response showed vascular congestion areas and infiltration of numerous inflammatory cells, mainly lymphocytes. The molecular and morphological approach supports the presence of three new lineages of clinostomid metacercariae in Argentina. This could lead to the discovery of a high number of lineages or species of Clinostomum from South America.

Keywords

ClinostomidsCOICichlidsCrenuchiidae
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e148
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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