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Performance of loop-mediated isothermal amplification (LAMP) for detection of Schistosoma mansoni infection compared with Kato–Katz and real-time PCR

Published online by Cambridge University Press:  18 April 2022

A.F. Allam Open the ORCID record for A.F. Allam [Opens in a new window] ,
M.A.-N. Kamel ,
H.F. Farag ,
H.G. Raheem ,
A.Y. Shehab  and
N.A.-E. Hagras
A.F. Allam*
Affiliation:
Department of Parasitology, Medical Research Institute, University of Alexandria, Alexandria, Egypt
M.A.-N. Kamel
Affiliation:
Department of Biochemistry, Medical Research Institute, University of Alexandria, Alexandria, Egypt
H.F. Farag
Affiliation:
Department of Parasitology, Medical Research Institute, University of Alexandria, Alexandria, Egypt
H.G. Raheem
Affiliation:
Department of Parasitology, Medical Research Institute, University of Alexandria, Alexandria, Egypt
A.Y. Shehab
Affiliation:
Department of Parasitology, Medical Research Institute, University of Alexandria, Alexandria, Egypt
N.A.-E. Hagras
Affiliation:
Department of Medical Laboratory Technology, Faculty of Applied Health Sciences Technology, Pharos University in Alexandria, Alexandria, Egypt
*
Author for correspondence: A.F. Allam, E-mail: amalalam2005@yahoo.com
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Abstract

The performance of loop-mediated isothermal amplification (LAMP) for detection of Schistosoma mansoni DNA from stool and urine samples in comparison with Kato–Katz and real-time polymerase chain reaction (PCR) was studied. After obtaining informed consent, 50 children participated in the present study and agreed to submit stool and urine samples. Stool samples were examined by Kato–Katz. Both real-time PCR and LAMP techniques were applied on stool and urine samples. The overall prevalence of S. mansoni was 46% in stool and urine samples as detected by the employed techniques, and 90% of cases had light infection intensity. The highest percentage of infection was diagnosed by real-time PCR (44%), followed by Kato–Katz (42%) and LAMP in the stool (36%), while the lowest percentages of infection were diagnosed by real-time PCR and LAMP in urine samples (24% and 14%, respectively). Kato–Katz, real-time PCR and LAMP showed 100% specificity where the sensitivity was 91.3%, 95.7% and 78.3%, respectively, in stool samples. Real-time PCR and LAMP showed lower sensitivity in urine samples. The LAMP assay is a promising technique for S. mansoni diagnosis in endemic countries of moderate and high-intensity infection. Yet, it needs further optimization, particularly in urine samples.

Keywords

Schistosoma mansoniKato–Katzreal-time PCRloop-mediated isothermal amplificationprimary school children
Type
Research Paper
Information
Journal of Helminthology , Volume 96 , 2022 , e28
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

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References

Abbasi, I, King, CH, Muchiri, EM and Hamburger, J (2010) Detection of Schistosoma mansoni and Schistosoma haematobium DNA by loop-mediated isothermal amplification: identification of infected snails from early prepatency. The American Journal of Tropical Medicine and Hygiene 83(2), 427.CrossRefGoogle ScholarPubMed
Abdul-Ghani, R, Al-Mekhlafi, AM and Karanis, P (2012) Loop-mediated isothermal amplification (LAMP) for malarial parasites of humans: would it come to clinical reality as a point-of-care test. Acta Tropica 122(3), 233–240.CrossRefGoogle ScholarPubMed
Ahmed, MU, Nahar, S, Safavieh, M and Zourob, M (2013) Real-time electrochemical detection of pathogen DNA using electrostatic interaction of a redox probe. The Analyst 138(3), 907–915.CrossRefGoogle ScholarPubMed
Allam, AF, Farag, HF, Zaki, A, Kader, O, Abdul-Ghani, R and Shehab, AY (2015) Detection of low-intensity Schistosoma mansoni infection by Percoll sedimentation and real-time PCR techniques in a low-endemicity Egyptian setting. Tropical Medicine & International Health 20, 658–664.CrossRefGoogle Scholar
Allam, AF, Farag, HF, Osman, MM, Hagras, NA, Ahmed, MA, Zaki, A, Ghani, RA and Shehab, AY (2018) Progress towards schistosomiasis elimination: application of sensitive diagnostic techniques. Primary Health Care 8, 305.Google Scholar
Allam, AF, Salem, A, Elsheredy, A, Dewair, MM, Ibrahim, HS, Farag, HF, Hagras, NA and Shehab, AY (2021a) Intestinal schistosomiasis among preschool and school-aged children in a rural setting near Alexandria: initiative for elimination. Tropical Medicine & International Health 26(6), 632–639.CrossRefGoogle Scholar
Allam, AF, Farag, HF, Lotfy, W, Fawzy, H, Elhadad, H and Shehab, A (2021b) Comparison among FLOTAC, Kato-Katz and formalin ether concentration techniques for diagnosis of intestinal parasitic infections in school children in an Egyptian rural setting. Parasitology 148(3), 289–294.CrossRefGoogle Scholar
Altman, GA (1992) Practical statistics for medical research. London, Chapman and Hall. pp. 404–408.Google Scholar
Bajiro, M, Dana, D, Ayana, M, Emana, D, Mekonnen, Z, Zawdie, B, Garbi, A, Kure, A and Zeynudin, A (2016) Prevalence of Schistosoma mansoni infection and the therapeutic efficacy of praziquantel among school children in Manna District, Jimma Zone, Southwest Ethiopia. Parasites & Vectors 9(1), 1–6.CrossRefGoogle ScholarPubMed
Besuschio, SA, Llano Murcia, M, Benatar, AF, et al. (2017) Analytical sensitivity and specificity of a loop-mediated isothermal amplification (LAMP) kit prototype for detection of Trypanosoma cruzi DNA in human blood samples. PLoS Neglected Tropical Diseases 11(7), e0005779.CrossRefGoogle ScholarPubMed
Cnops, L, Soentjens, P, Clerinx, J and Van Esbroeck, M (2013) A Schistosoma haematobium-specific real-time PCR for diagnosis of urogenital schistosomiasis in serum samples of international travelers and migrants. PLoS Neglected Tropical Diseases 7(8), e2413.CrossRefGoogle ScholarPubMed
Fernandez-Soto, P, Gandasegui Arahuetes, J, Sanchez Hernandez, A, Lopez Aban, J, Vicente Santiago, B and Muro, A (2014) A loop-mediated isothermal amplification (LAMP) assay for early detection of Schistosoma mansoni in stool samples: a diagnostic approach in a murine model. PLoS Neglected Tropical Diseases 8(9), e3126.CrossRefGoogle ScholarPubMed
Fernández-Soto, P, Gandasegui, J, Carranza Rodríguez, C, Pérez-Arellano, JL, Crego-Vicente, B, García-Bernalt Diego, J, López-Abán, J, Vicente, B and Muro, A (2019) Detection of Schistosoma mansoni-derived DNA in human urine samples by loop-mediated isothermal amplification (LAMP). PLoS One 14(3), e0214125.CrossRefGoogle Scholar
Fuss, A, Mazigo, HD, Tappe, D, Kasang, C and Mueller, A (2018) Comparison of sensitivity and specificity of three diagnostic tests to detect Schistosoma mansoni infections in school children in Mwanza region, Tanzania. PLoS One 13(8), e0202499.CrossRefGoogle ScholarPubMed
García-Bernalt, DJ, Fernández-Soto, P, Crego-Vicente, B, Alonso-Castrillejo, S, Febrer-Sendra, B, Gómez-Sánchez, A, Vicente, B, López-Abán, J and Muro, A (2019) Progress in loop-mediated isothermal amplification assay for detection of Schistosoma mansoni DNA: towards a ready-to-use test. Scientific Reports 9(1), 14744.CrossRefGoogle Scholar
Graeff-Teixeira, C, Favero, V, Pascoal, VF, et al. (2021) Low specificity of point-of-care circulating cathodic antigen (POCCCA) diagnostic test in a non-endemic area for Schistosomiasis mansoni in Brazil. Acta Tropica 217, 105863.CrossRefGoogle Scholar
Hajissa, K, Abd Elhafiz, MA, Eshag, HA, et al. (2018) Prevalence of schistosomiasis and associated risk factors among school children in Um-Asher Area, Khartoum, Sudan. BMC Research Notes 11(1), 1–5.CrossRefGoogle ScholarPubMed
Katz, N, Chaves, A and Pellegrino, J (1972) A simple device for quantitative stool thick-smear technique in Schistosomiasis mansoni. Rev Inst Med Trop Sao Paulo 14, 397–400.Google ScholarPubMed
King, CH (2010) Parasites and poverty: the case of schistosomiasis. Acta Tropica 113, 95–104.CrossRefGoogle ScholarPubMed
Meurs, L, Brienen, E, Mbow, M, Ochola, EA, Mboup, S, Karanja, DM, Secor, WE, Polman, K and van Lieshout, L (2015) Is PCR the next reference standard for the diagnosis of Schistosoma in stool? A comparison with microscopy in Senegal and Kenya. PLoS Neglected Tropical Diseases 9(7), e0003959.CrossRefGoogle Scholar
Montresor, A (2007) Arithmetic or geometric means of eggs per gram are not appropriate indicators to estimate the impact of control measures in helminth infections. Transactions of the Royal Society of Tropical Medicine and Hygiene 101, 773–776.CrossRefGoogle Scholar
Morris, U and Aydin-Schmidt, B (2021) Performance and application of commercially available loop-mediated isothermal amplification (LAMP) kits in malaria endemic and non-endemic settings. Diagnostics (Basel, Switzerland) 11(2), 336.Google ScholarPubMed
Motawea, SM, Soliman, M, El-Nemr, H, Elnahas, HA, Sultan, DM and Gabr, AM (2004) Why Schistosomiasis mansoni is still highly prevalent in rural Ezbas in Kafr El Sheikh. Egypt. Benha Medical Journal 21(3), 305–306.Google Scholar
Mwangi, IN, Agola, EL, Mugambi, RM, Shiraho, EA and Mkoji, GM (2018) Development and evaluation of a loop-mediated isothermal amplification assay for diagnosis of Schistosoma mansoni infection in faecal samples. Journal of Parasitology Research 14, 2018.Google Scholar
Okoyo, C, Simiyu, E, Njenga, SM and Mwandawiro, C (2018) Comparing the performance of circulating cathodic antigen and Kato-Katz techniques in evaluating Schistosoma mansoni infection in areas with low prevalence in selected counties of Kenya: a cross-sectional study. BMC Public Health 18(1), 478–485.CrossRefGoogle ScholarPubMed
Peralta, JM and Cavalcanti, MG (2018) Is POC-CCA a truly reliable test for schistosomiasis diagnosis in low endemic areas? The Trace Results Controversy. PLoS Neglected Tropical Diseases 12(11), e0006813.CrossRefGoogle ScholarPubMed
Sandoval, N, Siles-Lucas, M, Perez-Arellano, JL, Carranza, C, Puente, S, Lopez-Aban, J and Muro, A (2006) A new PCR-based approach for the specific amplification of DNA from different Schistosoma species applicable to human urine samples. Parasitology 133(5), 581–587.CrossRefGoogle ScholarPubMed
Ten Hove, RJ, Verweij, JJ, Vereecken, K, Polman, K, Dieye, L and van Lieshout, L (2008) Multiplex real-time PCR for the detection and quantification of Schistosoma mansoni and S. haematobium infection in stool samples collected in northern Senegal. Transactions of the Royal Society of Tropical Medicine and Hygiene 102(2), 179–185.CrossRefGoogle ScholarPubMed
Versalovic, J and Lupski, JR (2002) Molecular detection and genotyping of pathogens: more accurate and rapid answers. Trends in Microbiology 10(10), s15–s21.CrossRefGoogle ScholarPubMed
Vincent, M, Xu, Y and Kong, H (2004) Helicase-dependent isothermal DNA amplification. EMBO Reports 5(8), 795–800.CrossRefGoogle ScholarPubMed
WHO (2002) Prevention and control of schistosomiasis and soil-transmitted helminthiasis. Technical report series, No. 912; WHO_TRS_PDF, 912, 1–63.Google Scholar
WHO (2016) The use of loop-mediated isothermal amplification (TB-LAMP) for the diagnosis of pulmonary tuberculosis. Policy guidance. Available at: https://www.who.int/tb/publications/factsheet_tb_lamp.pdfwwGoogle Scholar
WHO (2022) Schistosomiasis. Available at: http://www.who.int/mediacentre/factsheets/fs115/en/Google Scholar
Yaren, O, Alto, BW, Gangodkar, PV, Ranade, SR, Patil, KN, Bradley, KM, Yang, Z, Phadke, N and Benner, SA (2017) Point of sampling detection of Zika virus within a multiplexed kit capable of detecting dengue and chikungunya. BMC Infectious Diseases 17(1), 293.CrossRefGoogle ScholarPubMed