Hostname: page-component-cd9895bd7-lnqnp Total loading time: 0 Render date: 2024-12-26T04:17:05.426Z Has data issue: false hasContentIssue false
  • English
  • Français

Specific gravity of Opisthorchis viverrini eggs

Published online by Cambridge University Press:  05 June 2009

T. Harnnoi ,
A. Wijit ,
N. Morakote ,
V. Pipitgool  and
W. Maleewong
T. Harnnoi*
Affiliation:
Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand
A. Wijit
Affiliation:
Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand
N. Morakote
Affiliation:
Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand
V. Pipitgool
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
W. Maleewong
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
*
*Fax: 66 53 217144, E-mail: tharnnoi@suandok01.medicine.cmu.ac.th
Get access Rights & Permissions [Opens in a new window]

Abstract

The specific gravity of the eggs of the liver fluke Opisthorchis viverrini was determined using a sucrose gradient centrifugation and found to range from 1.2713 to 1.3043. The peak egg count was located at the sucrose fraction with a specific gravity of 1.2814. An attempt to float eggs in saturated sodium nitrate solution, sp.gr. 1.4, failed. Examination of human stool specimens for O. viverrini eggs by simple flotation in saturated sodium nitrate solution and the formol-ether sedimentation technique revealed that the flotation technique was not as efficient as the sedimentation technique. It was suggested that the flotation techniques were inappropriate for the detection of O. viverrini eggs in faeces or contaminated soil.

Type
Research Note
Information
Journal of Helminthology , Volume 72 , Issue 4 , December 1998 , pp. 359 - 361
Copyright
Copyright © Cambridge University Press 1998

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

David, E.D. & Lindquist, W.D. (1982) Determination of the specific gravity of certain helminth eggs using a sucrose density gradient centrifugation. Journal of Parasitology 68, 916–919.CrossRefGoogle ScholarPubMed
Faust, E.C. & Russell, P.F. (1964) Craig and Faust's clinical parasitology. 7th edn. pp. 977–979. Philadelphia, Lea & Febiger.Google Scholar
Faust, E.C.Beaver, P.C. & Jung, R.C. (1968) Animal agents and vectors of human disease. pp. 404–406. 3rd edn.Philadelphia: Lea & Febiger.Google Scholar
Fleck, S.L. & Moody, A.H. (1988) Diagnostic techniques in medical parasitology. p. 13. London, Wright.Google Scholar
O'Lorcain, P. (1994) Prevalence of Toxocara canis in public playgrounds in the Dublin area of Ireland. Journal of Helminthology 68, 237–241.CrossRefGoogle ScholarPubMed
Phillipson, R.F. (1961) Flotation technique for Opisthorchis and Clonorchis eggs. Transactions of the Royal Society of Tropical Medicine and Hygiene 56, 174.CrossRefGoogle Scholar
Weast, R.C. (1984) (Ed.) CRC handbook of chemistry and physics. 65th edn. pp. D265, E360–361. Boca Raton, CRC Press.Google Scholar
Yamaguchi, T., Khamboonruang, C., Inaba, T., Huang, W.H., Ishida, K., Fujimaki, Y., Asano, H., Thitasuth, P. & Vajarasthira, S. (1982) Studies on intestinal parasitic infections in Chiang Mai Province, North Thailand. Japanese Journal of Parasitology 31, 447–459.Google Scholar