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Betadine has a ciliotoxic effect on ciliated human respiratory cells

Published online by Cambridge University Press:  03 November 2014

J H Kim ,
J Rimmer ,
N Mrad ,
S Ahmadzada  and
R J Harvey
J H Kim*
Affiliation:
Rhinology and Skull Base Research Group, St. Vincent's Centre for Applied Medical Research, Darlinghurst, New South Wales, Australia Faculty of Medicine, University of New South Wales, Kensington, New South Wales, Australia
J Rimmer
Affiliation:
St. Vincent's Hospital Sydney, Darlinghurst, New South Wales, Australia Woolcock Institute of Medical Research, University of Sydney, Glebe, New South Wales, Australia
N Mrad
Affiliation:
Rhinology and Skull Base Research Group, St. Vincent's Centre for Applied Medical Research, Darlinghurst, New South Wales, Australia
S Ahmadzada
Affiliation:
Rhinology and Skull Base Research Group, St. Vincent's Centre for Applied Medical Research, Darlinghurst, New South Wales, Australia Department of Medicine, University of Notre Dame Australia, Darlinghurst, New South Wales, Australia
R J Harvey
Affiliation:
Rhinology and Skull Base Research Group, St. Vincent's Centre for Applied Medical Research, Darlinghurst, New South Wales, Australia Faculty of Medicine, University of New South Wales, Kensington, New South Wales, Australia St. Vincent's Hospital Sydney, Darlinghurst, New South Wales, Australia The Australian School of Advanced Medicine, Macquarie University, Macquarie Park, New South Wales, Australia
*
Address for correspondence: Miss Joo Hye Kim, Rhinology and Skull Base Research Group, St. Vincent's Centre for Applied Medical Research (AMR), Level 8 Lowy Packer Building – St. Vincent's Research Precinct, 405 Liverpool Street, Darlinghurst, New South Wales 2010, Australia E-mail: jhgrace328@hotmail.com
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Abstract

Objective:

This study investigated the effect of Betadine on ciliated human respiratory epithelial cells.

Methods:

Epithelial cells from human sinonasal mucosa were cultured at the air–liquid interface. The cultures were tested with Hanks' balanced salt solution containing 10 mM HEPES (control), 100 µM ATP (positive control), 5 per cent Betadine or 10 per cent Betadine (clinical dose). Ciliary beat frequency was analysed using a high-speed camera on a computer imaging system.

Results:

Undiluted 10 per cent Betadine (n = 6) decreased the proportion of actively beating cilia over 1 minute (p < 0.01). Ciliary beat frequency decreased from 11.15 ± 4.64 Hz to no detectable activity. The result was similar with 5 per cent Betadine (n = 7), with no significant difference compared with the 10 per cent solution findings.

Conclusion:

Betadine, at either 5 and 10 per cent, was ciliotoxic. Caution should be applied to the use of topical Betadine solution on the respiratory mucosal surface.

Keywords

BetadinePovidone-IodineCiliary Beat FrequencyAir Liquid InterfaceRespiratory EpitheliumPrimary Cell CultureCilia
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 129 , Issue S1 , January 2015 , pp. S45 - S50
Copyright
Copyright © JLO (1984) Limited 2014 

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