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Role of sphingosine 1-phosphate receptor expression in eosinophils of patients with allergic rhinitis, and effect of topical nasal steroid treatment on this receptor expression

Published online by Cambridge University Press:  23 September 2008

T Mackle ,
S S Gendy ,
M Walsh ,
R McConn-Walsh ,
R W Costello  and
M-T Walsh
T Mackle*
Affiliation:
Department of Otorhinolaryngology, Beaumont Hospital, Dublin, Ireland
S S Gendy
Affiliation:
Department of Otorhinolaryngology, Beaumont Hospital, Dublin, Ireland
M Walsh
Affiliation:
Department of Otorhinolaryngology, Beaumont Hospital, Dublin, Ireland
R McConn-Walsh
Affiliation:
Department of Otorhinolaryngology, Beaumont Hospital, Dublin, Ireland
R W Costello
Affiliation:
Department of Medicine, Royal College of Surgeons in Ireland, Dublin, Ireland
M-T Walsh
Affiliation:
Department of Medicine, Royal College of Surgeons in Ireland, Dublin, Ireland
*
Address for correspondence: Dr Tara Mackle, Department of Otorhinolaryngology, Beaumont Hospital, Dublin 9, Ireland. E-mail: Mackle_tara@hotmail.com
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Abstract

Objective:

Recent research has indicated that sphingosine 1-phosphate plays a role in allergy. This study examined the effect of allergen challenge on the expression of sphingosine 1-phosphate receptors on the eosinophils of allergic rhinitis patients, and the effect of steroid treatment on this expression.

Study design:

A prospective, non-randomised study.

Methods:

The study had three parts. Firstly, sphingosine 1-phosphate receptor expression on the eosinophils of allergic rhinitis patients and control patients was determined. Secondly, sphingosine 1-phosphate receptor expression was quantified pre- and post-allergen challenge, before and after a short course of fluticasone propionate; all patients underwent symptom scoring and peak nasal inspiratory flow measurement pre- and post-allergen challenge, both before and after steroid or saline treatment. Thirdly, the effect of sphingosine 1-phosphate on eosinophil migration was examined.

Results:

The eosinophils of both allergic rhinitis patients and controls expressed sphingosine 1-phosphate1, 3, 4, and 5. Eosinophils from all allergic rhinitis patients demonstrated up-regulation in sphingosine 1-phosphate expression after allergen challenge. These changes were statistically very significant for sphingosine 1-phosphate1, 4, and 5, and moderately significant for sphingosine 1-phosphate3. Sphingosine 1-phosphate receptor expression up-regulation was abolished in the steroid-treated group after allergen challenge; however, the saline-treated group showed no change in sphingosine 1-phosphate receptor expression after allergen challenge. Peak nasal inspiratory flow scores were significantly diminished after allergen challenge prior to treatment, but not after a course of topical nasal steroids. Sphingosine 1-phosphate induced eosinophil chemotaxis was increased following allergen challenge in allergic rhinitis subjects.

Conclusions:

Local intranasal steroid therapy acts directly to block allergen-induced up-regulation of sphingosine 1-phosphate receptors on the peripheral eosinophils of allergic rhinitis patients, and this is coincident with post-challenge peak nasal inspiratory flow measurement improvements. These observations support the idea that such an increase in sphingosine 1-phosphate receptor expression is clinically relevant in allergic rhinitis, with potential consequences for eosinophil migration and survival.

Keywords

Sphingosine 1-PhosphateEosinophilsAllergic RhinitisCorticosteroids
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 122 , Issue 12 , December 2008 , pp. 1309 - 1317
Copyright
Copyright © JLO (1984) Limited 2008

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References

1Goetzl, EJ, Wang, W, McGiffert, C, Huang, MC, Graler, MH. Sphingosine 1-phosphate and its G protein-coupled receptors constitute a multifunctional immunoregulatory system. J Cell Biochem 2004;92:1104–14CrossRefGoogle ScholarPubMed
2Rosen, H, Goetzl, EJ. Sphingosine 1-phosphate and its receptors: an autocrine and paracrine network. Nat Rev Immunol 2005;5:560–70CrossRefGoogle ScholarPubMed
3Takuwa, Y, Takuwa, N, Sugimoto, N. The Edg family G protein-coupled receptors for lysophospholipids: their signaling properties and biological activities. J Biochem (Tokyo) 2002;131:767–71CrossRefGoogle ScholarPubMed
4Allende, ML, Sasaki, T, Kawai, H, Olivera, A, Mi, Y, Van Echten-Deckert, G et al. Mice deficient in sphingosine kinase 1 are rendered lymphopenic by FTY720. J Biol Chem 2004;279:52487–92CrossRefGoogle ScholarPubMed
5Matloubian, M, Lo, CG, Cinamon, G, Lesneski, MJ, Xu, Y, Brinkmann, V et al. Lymphocyte egress from thymus and peripheral lymphoid organs is dependent on S1P receptor 1. Nature 2004;427:355–60CrossRefGoogle ScholarPubMed
6Mandala, S, Hajdu, R, Bergstrom, J, Quackenbush, E, Xie, J, Milligan, J et al. Alteration of lymphocyte trafficking by sphingosine-1-phosphate receptor agonists. Science 2002;296:346–9CrossRefGoogle ScholarPubMed
7Sawicka, E, Zuany-Amorim, C, Manlius, C, Trifilieff, A, Brinkmann, V, Kemeny, DM et al. Inhibition of Th1- and Th2-mediated airway inflammation by the sphingosine 1-phosphate receptor agonist FTY720. J Immunol 2003;171:6206–14CrossRefGoogle ScholarPubMed
8Cyster, JG. Chemokines, sphingosine 1-phosphate, and cell migration in secondary lymphoid organs. Ann Rev Immunol 2005;23:127–59CrossRefGoogle ScholarPubMed
9Jolly, PS, Bektas, M, Olivera, A, Gonzalez-Espinosa, C, Proia, RL, Rivera, J et al. Transactivation of sphingosine-1-phosphate receptors by FcepsilonRI triggering is required for normal mast cell degranulation and chemotaxis. J Exp Med 2004;199:959–70CrossRefGoogle ScholarPubMed
10Ammit, AJ, Hastie, AT, Edsall, LC, Hoffman, RK, Amrani, Y, Krymskaya, VP et al. Sphingosine 1-phosphate modulates human airway smooth muscle cell functions that promote inflammation and airway remodeling in asthma. Faseb J 2001;15:1212–14CrossRefGoogle ScholarPubMed
11Bousquet, JP, Van Cauwenberge, C, Bachert, GW, Canonica, P, Demoly, SR, Durham, W et al. European Academy of Allergy and Clinical Immunology (EAACI); Allergic rhinitis and its impact on asthma (ARIA). Requirements for medications commonly used in the treatment of allergic rhinitis. Allergy 2003;58:192–7CrossRefGoogle ScholarPubMed
12Meltzer, EO. Evaluating rhinitis: clinical, rhinomanometric, and cytologic assessments. J Allergy Clin Immunol 1988;82:900–8CrossRefGoogle ScholarPubMed
13Rahaman, M, Costello, RW, Belmonte, KE, Gendy, SS, Walsh, MT. Neutrophil sphingosine 1-phosphate and lysophosphatidic acid receptors in pneumonia. Am J Respir Cell Mol Biol 2006;34:233–41CrossRefGoogle ScholarPubMed
14Bradford, MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248–54CrossRefGoogle ScholarPubMed
15Kim, CS, Moon, BK, Jung, DH, Min, YG. Correlation between nasal obstruction symptoms and objective parameters of acoustic rhinometry and rhinomanometry. Auris Nasus Larynx 1998;25:45–8CrossRefGoogle ScholarPubMed
16Roithmann, R, Cole, P, Chapnik, J, Barreto, SM, Szalai, JP, Zamel, N. Acoustic rhinometry, rhinomanometry, and the sensation of nasal patency: a correlative study. J Otolaryngol 1994;23:454–8Google ScholarPubMed
17Gleeson, MJ, Youlten, LJ, Shelton, DM, Siodlak, MZ, Eiser, NM, Wengraf, CL. Assessment of nasal airway patency: a comparison of four methods. Clin Otolaryngol 1986;11:99–107CrossRefGoogle ScholarPubMed
18Jones, AS, Willatt, DJ, Durham, LM. Nasal airflow: resistance and sensation. J Laryngol Otol 1989;103:909–11CrossRefGoogle ScholarPubMed
19Hilberg, O. Objective measurement of nasal airway dimensions using acoustic rhinometry: methodological and clinical aspects. Allergy 2002;57(suppl 70):5–39CrossRefGoogle ScholarPubMed
20Hardcastle, PF, White, A, Prescott, RJ. Clinical and rhinometric assessment of the nasal airway – do they measure the same clinical entity? Clin Otol 1988;13:185–91CrossRefGoogle Scholar
21Eccles, R, Jawad, MS, Morris, S. The effects of oral administration of menthol on nasal resistance to airflow and nasal sensation of airflow in subjects suffering from nasal congestion associated with the common cold. J Pharm Pharmacol 1990;42:652–4CrossRefGoogle ScholarPubMed
22Kohno, T, Matsuyuki, H, Inagaki, Y, Igarashi, Y. Sphingosine 1-phosphate promotes cell migration through the activation of Cdc42 in Edg-6/S1P4-expressing cells. Genes Cells 2003;8:685–97CrossRefGoogle ScholarPubMed
23Graler, MH, Grosse, R, Kusch, A, Kremmer, E, Gudermann, T, Lipp, M. The sphingosine 1-phosphate receptor S1P4 regulates cell shape and motility via coupling to Gi and G12/13. J Cell Biochem 2003;89:507–19CrossRefGoogle ScholarPubMed
24Graeler, M, Goetzl, EJ. Activation-regulated expression and chemotactic function of sphingosine 1-phosphate receptors in mouse splenic T cells. FASEB J 2002;16:1874–78CrossRefGoogle ScholarPubMed