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Evaluation of epithelial proliferation in paediatric and adult cholesteatomas using the Ki-67 proliferation marker

Published online by Cambridge University Press:  14 December 2011

K Sikka ,
S C Sharma ,
A Thakar  and
S Dattagupta
K Sikka
Affiliation:
Department of Otorhinolaryngology and Head and Neck Surgery, All India Institute of Medical Sciences, New Delhi, India
S C Sharma*
Affiliation:
Department of Otorhinolaryngology and Head and Neck Surgery, All India Institute of Medical Sciences, New Delhi, India
A Thakar
Affiliation:
Department of Otorhinolaryngology and Head and Neck Surgery, All India Institute of Medical Sciences, New Delhi, India
S Dattagupta
Affiliation:
Department of Pathology, All India Institute of Medical Sciences, New Delhi, India
*
Address for correspondence: Professor S C Sharma, Head, Department of Otorhinolaryngology and Head and Neck Surgery, All India Institute of Medical Sciences, New Delhi 110029, India E-mail: suresh6sharma@yahoo.com
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Abstract

Introduction:

The aggressiveness of cholesteatoma in children compared with adults is well known. However, the factors influencing the poorer prognosis of paediatric cholesteatoma are not well understood. This study compared the proliferative potential of paediatric cholesteatoma with that of adult cholesteatoma, using Ki-67 as a proliferation marker.

Methods:

A prospective study of 67 patients with aural cholesteatoma was performed. Thirty-eight adult and 29 paediatric cases were evaluated using clinical parameters including bone erosion, complications and extent of disease. A surgical specimen underwent histological evaluation and measurement of the proliferation index using Ki-67 labelling. Normal epithelium from a control group was also examined.

Results:

Cholesteatoma epithelium has a greater rate of proliferation than normal skin. There were however no statistical differences between the paediatric and adult cholesteatoma groups in terms of clinical behaviour or proliferation potential. Paediatric cholesteatoma was similar to adult cholesteatoma in terms of complications, bone erosion and disease spread.

Conclusion:

Cholesteatoma is a disorder of epithelial proliferation. Although postulated to be more aggressive in children than adults, this study found no clinicopathological differences between paediatric and adult cases.

Keywords

CholesteatomaChildCellular ProliferationPathology
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 126 , Issue 5 , May 2012 , pp. 460 - 463
Copyright
Copyright © JLO (1984) Limited 2011

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References

1Jahn, AF. Cholesteatoma: what is it, how did it get there, and how do we get rid of it?. Otolaryngol Clin North Am 1989;22:847–57Google Scholar
2Tanaka, Y, Kojima, H, Miyazaki, H, Koga, T, Moriyama, H. Roles of cytokines and cell cycle regulating substances in proliferation of cholesteatoma epithelium. Laryngoscope 1999;109:1102–7Google Scholar
3Park, K, Park, HJ, Chun, YM. Immunohistochemical study on proliferative activity of experimental cholesteatoma. Eur Arch Otorhinolaryngol 2001;258:101–5CrossRefGoogle Scholar
4Bujia, J, Sudhoff, H, Holly, A, Hildmann, H, Kastenbauer, E. Immunohistochemical detection of proliferation cell nuclear antigen in middle ear cholesteatoma. Eur Arch Otorhinolaryngol 1996;253:21–4CrossRefGoogle ScholarPubMed
5Cattoretti, G, Becker, MH, Key, G, Duchrow, M, Schlüter, C, Galle, J et al. Monoclonal antibodies against recombinant parts of the Ki-67 antigen (MIB 1 and MIB 3) detect proliferating cells in microwave-processed formalin-fixed paraffin sections. J Pathol 1992;168:357–63CrossRefGoogle ScholarPubMed
6Ergun, S, Carlsoo, B, Zheung, X. Apoptosis in meatal skin, cholesteatoma and squamous cell carcinoma of the ear. Clin Otolaryngol 1999;24:280–5CrossRefGoogle ScholarPubMed
7Mallet, Y, Nouwen, J, Lecomte-Houcke, M, Desaulty, A. Aggressiveness and quantification of epithelial proliferation of middle ear cholesteatoma by MIB 1. Laryngoscope 2003;113:328–31CrossRefGoogle Scholar
8Bujia, J, Holly, A, Antoli Candela, F, Tapia, MF, Kastenbauer, E. Immunobiological peculiarities of cholesteatoma in children: quantification of epithelial proliferation by MIB 1. Laryngoscope 1996;106:865–8CrossRefGoogle Scholar
9Sudhoff, H, Bujia, J, Fisseler-Eckhoff, A, Holly, A, Schulz-Flake, C, Hildmann, H. Expression of a cell cycle associated nuclear antigen (MIB 1) in cholesteatoma and auditory meatal skin. Laryngoscope 1995;105:1227–31Google Scholar
10Dornelles, C, Costa, SS, Meurer, L, Schweiger, C. Some considerations about acquired adult and pediatric cholesteatomas. Braz J Otorinolaringol 2005;71:536–46Google Scholar
11Quaranta, A, Ressa, L, Santangelo, A. Otomastoid cholesteatoma in children: histopathological findings. Int J Pediatric Otorhinolaryngol 1986;12:121–6Google Scholar
12Dornelles, C, Meurer, L, Selaimen da Costa, S, Schweiger, C. Histologic description of acquired cholesteatomas: comparison between children and adults. Braz J Otorhinolaryngol 2006;72:641–8CrossRefGoogle ScholarPubMed
13Dornelles Cde, C, da Costa, SS, Meurer, L, Rosito, LP, da Silva, AR, Alves, SL. Comparison of acquired cholesteatoma between pediatric and adult patients. Eur Arch Otorhinolaryngol 2009;266:1553–61Google Scholar
14James, AL, Chadha, NK, Papsin, BC, Stockley, TL. Pediatric cholesteatoma and variants in the gene encoding connexin 26. Laryngoscope 2010;120:183–7CrossRefGoogle ScholarPubMed