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Comparative study on the influence of the content and functionalization of alginate matrices on K-562 cell viability and differentiation

Published online by Cambridge University Press:  19 May 2020

Everton C. Morais ,
Helena T. Schroeder ,
Cristina S. Souza ,
Silvia R. Rodrigues ,
Maria Ines L. Rodrigues ,
Paulo Ivo Homem de Bittencourt Jr.  and
João Henrique Z. Dos Santos Open the ORCID record for João Henrique Z. Dos Santos [Opens in a new window]
Everton C. Morais
Affiliation:
Instituto de Química, Universidade Federal do Rio Grande do Sul, Porto Alegre 91500-000, Brazil
Helena T. Schroeder
Affiliation:
Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre 90046-902, Brazil
Cristina S. Souza
Affiliation:
Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre 90046-902, Brazil
Silvia R. Rodrigues
Affiliation:
Instituto de Química, Universidade Federal do Rio Grande do Sul, Porto Alegre 91500-000, Brazil
Maria Ines L. Rodrigues
Affiliation:
Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre 90046-902, Brazil
Paulo Ivo Homem de Bittencourt Jr.*
Affiliation:
Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre 90046-902, Brazil
João Henrique Z. Dos Santos*
Affiliation:
Instituto de Química, Universidade Federal do Rio Grande do Sul, Porto Alegre 91500-000, Brazil
*
a)Address all correspondence to these authors. e-mail: pauloivo@ufrgs.br
b)e-mail: jhzds@iq.ufrgs.br
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Abstract

Microencapsulation of functioning cells for transplantation therapies is particularly promising, but the cells must retain their proper physiology and viability after being encapsulated. K-562 cells are multipotential and exhibit erythroid, megakaryocytic, or granulocytic properties that can be exploited by using an array of physiologically differentiating factors. The potential for cell differentiation makes it attractive the use of K-562 cells as functional model to the assessment of the effects of encapsulation on cell viability and physiology. Thus, alginate and hybrid alginate matrices were produced by extrusion technique for K-562 cell encapsulation. The produced systems were composed of bare alginate (1–3 wt%) and alginate in combination with chitosan or silica. The resulting materials were characterized by dynamic laser scattering, zeta potential measurements, small-angle X-ray scattering, and Fourier transform infrared spectroscopy. To assess viability, the encapsulated cells were subjected to the Trypan blue exclusion technique and NAD(P)H-dependent oxidoreductase (MTT) assays; hemin-induced erythroid differentiation capacity was also evaluated. The encapsulated alginate-based systems were shown to be monomodal and bimodal, depending on the nature of the capsule, with mean sizes in the range between 414 and 4.129 nm. Encapsulated cells exhibited viability ratios compatible with their use for prolonged cell cultures. Erythroid differentiation occurred in the range between 39 and 44%. The present results allow the consideration of the viability of therapeutic cells encapsulated in both bare alginate and in hybrid matrices.

Keywords

encapsulationalginatecell viabilityerythroid differentiation
Type
Article
Information
Journal of Materials Research , Volume 35 , Issue 10 , 28 May 2020 , pp. 1249 - 1261
Copyright
Copyright © Materials Research Society 2020

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