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Electrodeposition of bactericidal and bioactive nano-hydroxyapatite onto electrospun piezoelectric polyvinylidene fluoride scaffolds

Published online by Cambridge University Press:  16 November 2020

Pedro J. G. Rodrigues ,
Conceição de M. V. Elias ,
Bartolomeu C. Viana ,
Luciana M. de Hollanda ,
Thiago D. Stocco ,
Luana M. R. de Vasconcellos ,
Daphne de C. R. Mello ,
Francisco E. P. Santos ,
Fernanda R. Marciano  and
Anderson O. Lobo Open the ORCID record for Anderson O. Lobo [Opens in a new window]
Pedro J. G. Rodrigues
Affiliation:
LIMAV — Interdisciplinary Laboratory for Advanced Materials, BioMatLab Group, Materials Science & Engineering Graduate Program, UFPI — Federal University of Piauí, 64049-550Teresina, Piauí, Brazil
Conceição de M. V. Elias
Affiliation:
Instituto Científico e Tecnológico, Universidade Brasil, 08230-030 São Paulo, Brazil
Bartolomeu C. Viana
Affiliation:
LIMAV — Interdisciplinary Laboratory for Advanced Materials, BioMatLab Group, Materials Science & Engineering Graduate Program, UFPI — Federal University of Piauí, 64049-550Teresina, Piauí, Brazil Department of Physics, UFPI — Federal University of Piauí, 64049-550Teresina, PI, Brazil
Luciana M. de Hollanda
Affiliation:
Universidade UniMetrocamp, 13035-500 Campinas, São Paulo, Brazil Faculty of Medical Sciences, State University of Campinas, 13083-970 Campinas, São Paulo, Brazil
Thiago D. Stocco
Affiliation:
Faculty of Medical Sciences, State University of Campinas, 13083-970 Campinas, São Paulo, Brazil University of Santo Amaro, 04829-300São Paulo, Brazil
Luana M. R. de Vasconcellos
Affiliation:
Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, Sao Paulo State University, 12247-004 Sao Jose dos Campos, Sao Paulo, Brazil
Daphne de C. R. Mello
Affiliation:
Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, Sao Paulo State University, 12247-004 Sao Jose dos Campos, Sao Paulo, Brazil
Francisco E. P. Santos
Affiliation:
LIMAV — Interdisciplinary Laboratory for Advanced Materials, BioMatLab Group, Materials Science & Engineering Graduate Program, UFPI — Federal University of Piauí, 64049-550Teresina, Piauí, Brazil Department of Physics, UFPI — Federal University of Piauí, 64049-550Teresina, PI, Brazil
Fernanda R. Marciano
Affiliation:
Department of Physics, UFPI — Federal University of Piauí, 64049-550Teresina, PI, Brazil
Anderson O. Lobo*
Affiliation:
LIMAV — Interdisciplinary Laboratory for Advanced Materials, BioMatLab Group, Materials Science & Engineering Graduate Program, UFPI — Federal University of Piauí, 64049-550Teresina, Piauí, Brazil
*
a)Address all correspondence to this author. e-mail: lobo@ufpi.edu.br
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Abstract

The fibrous scaffolds for bone tissue engineering that mimic the extracellular matrix with bioactive and bactericidal properties could provide adequate conditions for regeneration of damaged bone. Electrospun ultrathin fiber covered with nano-hydroxyapatite is a favorable fibrous scaffold design. We developed a fast and reproducible strategy to produce polyvinylidene fluoride (PVDF)/nano-hydroxyapatite (nHAp) nanofibrous scaffolds with bactericidal and bioactive properties. Fibrous PVDF scaffolds were obtained first by the electrospinning method. Then, their surfaces were modified using oxygen plasma treatment followed by electrodeposition of nHAp. This process formed nanofibrous and superhydrophilic PVDF fibers (133.6 nm, fiber average diameter) covered with homogeneous nHAp (202.6 nm, average particle diameter) crystals. Energy-dispersive X-ray spectrometry demonstrated the presence of calcium phosphate, indicating a Ca/P molar ratio of approximately 1.64. X-ray diffraction, Fourier transform infrared spectroscopy, and Raman spectroscopy spectra identified β-phase of nHAp. Thermal analysis indicated a slight reduction in stability after nHAp electrodeposition. Bactericidal assays showed that nHAp exhibited 99.8% efficiency against Pseudomonas aeruginosa bacteria. The PVDF/Plasma and PVDF/nHAp groups had the highest cell viability, total protein, and alkaline phosphatase activity by 7 days after exposure of the scaffolds to MG63 cell culture. Therefore, the developed scaffolds are an exciting alternative for application in bone regeneration.

Keywords

polyvinylidene fluoridenano-hydroxyapatitenanotechnologyelectrospinningelectrodepositionbactericidalbone regeneration
Type
Article
Information
Journal of Materials Research , Volume 35 , Issue 23-24 , 14 December 2020 , pp. 3265 - 3275
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Copyright
Copyright © The Author(s), 2020, published on behalf of Materials Research Society by Cambridge University Press

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