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Molecular data delineate cryptic Nassarius species and characterize spatial genetic structure of N. nitidus

Published online by Cambridge University Press:  13 July 2011

L. Couceiro ,
L. López ,
E.E. Sotka ,
J.M. Ruiz  and
R. Barreiro
L. Couceiro
Affiliation:
Área de Ecoloxía, Facultade de Ciencias, Universidade da Coruña, Campus da Zapateira s/n, 15071-A Coruña, Spain
L. López
Affiliation:
Área de Ecoloxía, Facultade de Ciencias, Universidade da Coruña, Campus da Zapateira s/n, 15071-A Coruña, Spain
E.E. Sotka
Affiliation:
Grice Marine Laboratory, College of Charleston, 205 Fort Johnson Road, Charleston, SC-29412, USA
J.M. Ruiz
Affiliation:
Área de Ecoloxía, Facultade de Ciencias, Universidade da Coruña, Campus da Zapateira s/n, 15071-A Coruña, Spain
R. Barreiro*
Affiliation:
Área de Ecoloxía, Facultade de Ciencias, Universidade da Coruña, Campus da Zapateira s/n, 15071-A Coruña, Spain
*
Correspondence should be addressed to: R. Barreiro, Área de Ecoloxía, Facultade de Ciencias, Universidade da Coruña, Campus da Zapateira s/n, 15071-A Coruña, Spain email: rodbar@udc.es
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Abstract

Nassarius nitidus is a marine snail species with a widespread distribution along European shorelines from the North Sea to the Mediterranean and Black Seas. Despite its widespread distribution, N. nitidus has been largely neglected due to taxonomic confusion with the congeneric Nassarius reticulatus. Discrimination between these two Nassarius is particularly challenging in areas where their ranges overlap: the European Atlantic and western Mediterranean. Here, we propose the use of the mtDNA gene cytochrome c oxidase subunit I (COI) to discriminate between these two morphologically similar congeneric species. A numerically comprehensive sampling of the areas of overlap reveals strong population-level differentiation in N. nitidus, particularly between the Mediterranean and the Atlantic. Despite this strong population structure, we found a wide (9.6 ± 1.6% mean K2P corrected sequence distance) barcoding gap with N. reticulatus that guarantees that COI barcodes may serve as a reliable diagnostic tool. A protocol using species-specific restriction patterns was developed to allow a quick and accurate discrimination between these two cryptic species.

Keywords

Nassarius reticulatusNassarius nitiduscryptic speciesDNA barcodingmtDNACOI
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 92 , Issue 5 , August 2012 , pp. 1175 - 1182
Copyright
Copyright © Marine Biological Association of the United Kingdom 2011

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